Molecular cloning of gene fragment encoding 4-coumarate: Coenzyme A ligase of Sengon (Paraserianthes falcataria)
| Main Authors: | Hartati, Sri N., Sudarmonowati, Enny, Suharsono, S., Sofyan, Kurnia |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Gadjah Mada
, 2015
|
| Online Access: |
https://jurnal.ugm.ac.id/ijbiotech/article/view/7816 https://jurnal.ugm.ac.id/ijbiotech/article/view/7816/6057 |
| ctrlnum |
article-7816 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Molecular cloning of gene fragment encoding 4-coumarate: Coenzyme A ligase of Sengon (Paraserianthes falcataria)</title><creator>Hartati, Sri N.</creator><creator>Sudarmonowati, Enny</creator><creator>Suharsono, S.</creator><creator>Sofyan, Kurnia</creator><subject lang="en-US"/><description lang="en-US">4-coumarate:Coenzyme A ligase (4CL) plays an important role in lignin biosynthetic pathway thatcatalyzed the activation of coumaric acid, caffeic acid or ferulic acid to be a syringil monomer. Ligninbiosynthesis control through 4CL down regulating would support lower lignin wood production. Theobjective of this study was to clone conserved region cDNA of gene encoding 4CL. Gene fragment isolation wasconducted by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerateheterologous primer. The RT-PCR products were purified, sequenced and analyzed to select the highlyhomologous fragment to 4CL. BLASTanalysis result showed that deduction of amino acid sequences from oneof two RT-PCR product nucleotide was highly homologous with the 4CL conserved region from Rubbus ideaus,Oryza sativa, Populus tomentosa, Populus balsamifera, Betulla platyphilla, Nicotiana tabacum, and Arabidopsisthaliana with identity ranging from 78-90%.Key words: 4-coumarate: Coenzyme A ligase, lignin, sengon</description><publisher lang="en-US">Universitas Gadjah Mada</publisher><contributor lang="en-US"/><date>2015-11-09</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>https://jurnal.ugm.ac.id/ijbiotech/article/view/7816</identifier><identifier>10.22146/ijbiotech.7816</identifier><source lang="en-US">Indonesian Journal of Biotechnology; Vol 15, No 1 (2010)</source><source>2089-2241</source><source>0853-8654</source><language>eng</language><relation>https://jurnal.ugm.ac.id/ijbiotech/article/view/7816/6057</relation><rights lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</rights><recordID>article-7816</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Hartati, Sri N. Sudarmonowati, Enny Suharsono, S. Sofyan, Kurnia |
| title |
Molecular cloning of gene fragment encoding 4-coumarate: Coenzyme A ligase of Sengon (Paraserianthes falcataria) |
| publisher |
Universitas Gadjah Mada |
| publishDate |
2015 |
| url |
https://jurnal.ugm.ac.id/ijbiotech/article/view/7816 https://jurnal.ugm.ac.id/ijbiotech/article/view/7816/6057 |
| contents |
4-coumarate:Coenzyme A ligase (4CL) plays an important role in lignin biosynthetic pathway thatcatalyzed the activation of coumaric acid, caffeic acid or ferulic acid to be a syringil monomer. Ligninbiosynthesis control through 4CL down regulating would support lower lignin wood production. Theobjective of this study was to clone conserved region cDNA of gene encoding 4CL. Gene fragment isolation wasconducted by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerateheterologous primer. The RT-PCR products were purified, sequenced and analyzed to select the highlyhomologous fragment to 4CL. BLASTanalysis result showed that deduction of amino acid sequences from oneof two RT-PCR product nucleotide was highly homologous with the 4CL conserved region from Rubbus ideaus,Oryza sativa, Populus tomentosa, Populus balsamifera, Betulla platyphilla, Nicotiana tabacum, and Arabidopsisthaliana with identity ranging from 78-90%.Key words: 4-coumarate: Coenzyme A ligase, lignin, sengon |
| id |
IOS1080.article-7816 |
| institution |
Universitas Gadjah Mada |
| institution_id |
19 |
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library:university library |
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Perpustakaan Pusat Universitas Gadjah Mada |
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488 |
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Indonesian Journal of Biotechnology |
| repository_id |
1080 |
| subject_area |
Bioteknology medicine and health agriculture biology |
| city |
SLEMAN |
| province |
DAERAH ISTIMEWA YOGYAKARTA |
| repoId |
IOS1080 |
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2017-01-09T04:30:36Z |
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2017-01-09T04:41:36Z |
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