Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris
| Main Author: | Husin, Bahagiawati A.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111 |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
, 2016
|
| Subjects: | |
| Online Access: |
http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709 http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709/3085 |
| ctrlnum |
article-3709 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris</title><creator>Husin, Bahagiawati A.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111</creator><subject lang="en-US">α-amylase inhibitor, common bean, storage pest Callosobruchus maculatus</subject><description lang="en-US">Plant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean (Phaseolus vulgaris) contain a glycoprotein that inhibits activity of α-amylase of insects. A α-amylase inhibitor was purified from the common bean seeds. The purified α-amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus. The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α-amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects.</description><publisher lang="en-US">Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian</publisher><contributor lang="en-US"/><date>2016-08-05</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709</identifier><identifier>10.21082/jbio.v1n1.2005.p7-12</identifier><source lang="en-US">Jurnal AgroBiogen; Vol 1, No 1 (2005): April; 7-12</source><source>2549-1547</source><source>1907-1094</source><language>eng</language><relation>http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709/3085</relation><rights lang="en-US">Copyright (c) 2016 Jurnal AgroBiogen</rights><recordID>article-3709</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Husin, Bahagiawati A.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111 |
| title |
Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris |
| publisher |
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian |
| publishDate |
2016 |
| topic |
α-amylase inhibitor common bean storage pest Callosobruchus maculatus |
| url |
http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709 http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709/3085 |
| contents |
Plant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean (Phaseolus vulgaris) contain a glycoprotein that inhibits activity of α-amylase of insects. A α-amylase inhibitor was purified from the common bean seeds. The purified α-amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus. The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α-amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects. |
| id |
IOS389.article-3709 |
| institution |
Kementrian Pertanian |
| institution_id |
72 |
| institution_type |
library:special library |
| library |
Perpustakaan Kementrian Pertanian |
| library_id |
123 |
| collection |
Jurnal AgroBiogen |
| repository_id |
389 |
| city |
JAKARTA SELATAN |
| province |
DKI JAKARTA |
| repoId |
IOS389 |
| first_indexed |
2016-09-24T00:24:13Z |
| last_indexed |
2017-02-25T09:49:15Z |
| recordtype |
dc |
| merged_child_boolean |
1 |
| _version_ |
1722457929032925184 |
| score |
17.60506 |