The synergistic effect of doxorubicin and ethanolic extracts of Caesalpinia sappan L. wood and Ficus septica Burm. f. leaves on viability, cell cycle progression, and apoptosis induction of MCF7 cells
| Main Authors: | Haryanti, Sari, Pramono, Suwijiyo, Murwanti, Retno, Meiyanto, Edy |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Gadjah Mada
, 2016
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| Subjects: | |
| Online Access: |
https://jurnal.ugm.ac.id/ijbiotech/article/view/26105 https://jurnal.ugm.ac.id/ijbiotech/article/view/26105/16533 |
| ctrlnum |
article-26105 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">The synergistic effect of doxorubicin and ethanolic extracts of Caesalpinia sappan L. wood and Ficus septica Burm. f. leaves on viability, cell cycle progression, and apoptosis induction of MCF­7 cells</title><creator>Haryanti, Sari</creator><creator>Pramono, Suwijiyo</creator><creator>Murwanti, Retno</creator><creator>Meiyanto, Edy</creator><subject lang="en-US">apoptosis; cell cycle; doxorubicin; Ficus septica leaves; MCF-7, sappan wood</subject><description lang="en-US">Caesalpinia sappan L. and Ficus septica Burm. f  known asa potential plant with wide variety of medicinal properties, including anticancer. Present study was aimed to explore cytotoxic effect ofsappan wood (ECS) and awar-awar leaves (EFS), and its combination with doxorubicin (dox) on MCF-7 cells focusing on cell cycle progression and apoptosis induction.The result of MTT assay showed that single treatment of ECS and dox performed cytotoxic effect with the IC50 value of 32 µg/mL and 6 µM respectively, while EFS performed low cytotoxic effect with the IC50 value of 282 µg/mL. The combination of ECS with EFS and doxorubicin showed synergistic cytotoxic effect. Flow cytometry analysis revealed that combination of ECS (16 µg/mL) with EFS (8 µg/mL) and doxorubicin (2 µM) induced apoptosis, and cell accumulation at sub-G1 and G2/M phases.Immunoblotting assay confirmed the apoptosis induction of this combination through increasing of cleavage of PARP-1. Based on these results, the synergistic cytotoxic effect of this combinationwas through G2/M phase accumulation and apoptosis inductionand potentially to be developed as co-chemotherapeutic agent.</description><publisher lang="en-US">Universitas Gadjah Mada</publisher><contributor lang="en-US"/><date>2016-06-01</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>https://jurnal.ugm.ac.id/ijbiotech/article/view/26105</identifier><identifier>10.22146/ijbiotech.26105</identifier><source lang="en-US">Indonesian Journal of Biotechnology; Vol 21, No 1 (2016); 29-37</source><source>2089-2241</source><source>0853-8654</source><language>eng</language><relation>https://jurnal.ugm.ac.id/ijbiotech/article/view/26105/16533</relation><rights lang="en-US">Copyright (c) 2016 The Author(s)</rights><rights lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</rights><recordID>article-26105</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Haryanti, Sari Pramono, Suwijiyo Murwanti, Retno Meiyanto, Edy |
| title |
The synergistic effect of doxorubicin and ethanolic extracts of Caesalpinia sappan L. wood and Ficus septica Burm. f. leaves on viability, cell cycle progression, and apoptosis induction of MCF7 cells |
| publisher |
Universitas Gadjah Mada |
| publishDate |
2016 |
| topic |
apoptosis; cell cycle; doxorubicin; Ficus septica leaves; MCF-7 sappan wood |
| url |
https://jurnal.ugm.ac.id/ijbiotech/article/view/26105 https://jurnal.ugm.ac.id/ijbiotech/article/view/26105/16533 |
| contents |
Caesalpinia sappan L. and Ficus septica Burm. f known asa potential plant with wide variety of medicinal properties, including anticancer. Present study was aimed to explore cytotoxic effect ofsappan wood (ECS) and awar-awar leaves (EFS), and its combination with doxorubicin (dox) on MCF-7 cells focusing on cell cycle progression and apoptosis induction.The result of MTT assay showed that single treatment of ECS and dox performed cytotoxic effect with the IC50 value of 32 μg/mL and 6 μM respectively, while EFS performed low cytotoxic effect with the IC50 value of 282 μg/mL. The combination of ECS with EFS and doxorubicin showed synergistic cytotoxic effect. Flow cytometry analysis revealed that combination of ECS (16 μg/mL) with EFS (8 μg/mL) and doxorubicin (2 μM) induced apoptosis, and cell accumulation at sub-G1 and G2/M phases.Immunoblotting assay confirmed the apoptosis induction of this combination through increasing of cleavage of PARP-1. Based on these results, the synergistic cytotoxic effect of this combinationwas through G2/M phase accumulation and apoptosis inductionand potentially to be developed as co-chemotherapeutic agent. |
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IOS1080.article-26105 |
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Universitas Gadjah Mada |
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19 |
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library:university library |
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Perpustakaan Pusat Universitas Gadjah Mada |
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488 |
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Indonesian Journal of Biotechnology |
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1080 |
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Bioteknology medicine and health agriculture biology |
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SLEMAN |
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DAERAH ISTIMEWA YOGYAKARTA |
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IOS1080 |
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2017-07-31T19:33:40Z |
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2018-11-19T22:20:23Z |
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