POLA DISTRIBUSI MITOKONDRIA SEL-SEL TROFOBLAS BLASTOSIS MENCIT (Mus Muculus Albinus) DAN PENGARUHNYA TERHADAP KEGAGALAN HATCHING DAN IMPLANTASI
| Main Authors: | Helmita, Roza, Djuwita, Ita, Purwantara, Bambang |
|---|---|
| Other Authors: | IAIN Batusangkar |
| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
IAIN Batusangkar
, 2016
|
| Online Access: |
http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121 http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121/121 |
| ctrlnum |
article-121 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">POLA DISTRIBUSI MITOKONDRIA SEL-SEL TROFOBLAS BLASTOSIS MENCIT (Mus Muculus Albinus) DAN PENGARUHNYA TERHADAP KEGAGALAN HATCHING DAN IMPLANTASI</title><creator>Helmita, Roza</creator><creator>Djuwita, Ita</creator><creator>Purwantara, Bambang</creator><subject lang="id-ID"/><subject lang="id-ID"/><description lang="id-ID">Implantation is the most critical stage in the establishment of pregnancy. In mammals, it has been estimated that between 25% and 60% of conceptuses are lost before or at the time of implantation. The objectives of  this study were to investigate  the ability of blastocyst hatching and attachment of trophoblast cells in in vitro, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst, the activity of mitochondria NADH-CoQ reductase and the pattern of mitochondrial distribution. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEM medium in 5% CO2 incubator at 37°C for 10 days. The trophoblasts monolayer were processed for Giemsa staining and histochemistry analysis of NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed the ability of blastocyst hatching in in vitro were different. The trophoblast outgrowth diameter of 24 h hatched blastocyst was significantly different with the 48 h hatched and non hatched blastocyst. The activity of NADH-CoQ reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05) and the distribution of mitochondrial in trophoblast cell cytoplasma of 24 h and  48 h hatched blastocyst were homogen around nucleus whereas thoes of non hatched blastocyst were cluster and heterogen. In conclusion, in in vitro study the failure of blastocysts hatching and implantation was due to the failure of outgrowth and differentiation of the trofoblast cells and the impairment of NADH-CoQ reductase activity in complex I mitochondria to produce energy and the pattern of mitochondrial distribution.Key words: mitochondrial distribution, NADH- tetrazolium reductase, trophoblast, implantation </description><publisher lang="en-US">IAIN Batusangkar</publisher><contributor lang="id-ID">IAIN Batusangkar</contributor><date>2016-09-23</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121</identifier><identifier>10.31958/js.v7i1.121</identifier><source lang="id-ID">Sainstek; Vol 7, No 1 (2015); 16-25</source><source lang="en-US">Sainstek : Jurnal Sains dan Teknologi; Vol 7, No 1 (2015); 16-25</source><source>2580-278X</source><source>2085-8019</source><source>10.31958/js.v7i1</source><language>ind</language><relation>http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121/121</relation><rights lang="en-US">Copyright (c) 2016 Roza Helmita, Ita Djuwita, Bambang Purwantara</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc-nd/4.0</rights><recordID>article-121</recordID></dc>
|
| language |
ind |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:Journal |
| author |
Helmita, Roza Djuwita, Ita Purwantara, Bambang |
| author2 |
IAIN Batusangkar |
| title |
POLA DISTRIBUSI MITOKONDRIA SEL-SEL TROFOBLAS BLASTOSIS MENCIT (Mus Muculus Albinus) DAN PENGARUHNYA TERHADAP KEGAGALAN HATCHING DAN IMPLANTASI |
| publisher |
IAIN Batusangkar |
| publishDate |
2016 |
| url |
http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121 http://ecampus.iainbatusangkar.ac.id/ojs/index.php/sainstek/article/view/121/121 |
| contents |
Implantation is the most critical stage in the establishment of pregnancy. In mammals, it has been estimated that between 25% and 60% of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the ability of blastocyst hatching and attachment of trophoblast cells in in vitro, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst, the activity of mitochondria NADH-CoQ reductase and the pattern of mitochondrial distribution. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEM medium in 5% CO2 incubator at 37°C for 10 days. The trophoblasts monolayer were processed for Giemsa staining and histochemistry analysis of NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed the ability of blastocyst hatching in in vitro were different. The trophoblast outgrowth diameter of 24 h hatched blastocyst was significantly different with the 48 h hatched and non hatched blastocyst. The activity of NADH-CoQ reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05) and the distribution of mitochondrial in trophoblast cell cytoplasma of 24 h and 48 h hatched blastocyst were homogen around nucleus whereas thoes of non hatched blastocyst were cluster and heterogen. In conclusion, in in vitro study the failure of blastocysts hatching and implantation was due to the failure of outgrowth and differentiation of the trofoblast cells and the impairment of NADH-CoQ reductase activity in complex I mitochondria to produce energy and the pattern of mitochondrial distribution.Key words: mitochondrial distribution, NADH- tetrazolium reductase, trophoblast, implantation |
| id |
IOS12993.article-121 |
| institution |
IAIN BATUSANGKAR |
| affiliation |
onesearch.perpusnas.go.id ptki.onesearch.id |
| institution_id |
2828 |
| institution_type |
library:university library |
| library |
Perpustakaan IAIN Batusangkar |
| library_id |
3170 |
| collection |
Sainstek : Jurnal Sains dan Teknologi |
| repository_id |
12993 |
| subject_area |
Science and Religion/Sains, Ilmu Pengetahuan dan Agama Religious Education/Pendidikan Keagamaan, Pendidikan Agama Higher Education in Indonesia/Pendidikan Tinggi di Indonesia, Perguruan Tinggi di Indonesia |
| city |
TANAH DATAR |
| province |
SUMATERA BARAT |
| shared_to_ipusnas_str |
1 |
| repoId |
IOS12993 |
| first_indexed |
2020-01-16T05:49:49Z |
| last_indexed |
2020-01-16T07:29:03Z |
| recordtype |
dc |
| _version_ |
1686648102608437248 |
| score |
17.607244 |