UJI COBA BACILLUS THURINGIENSIS H-14 TERHADAP JENTIK NYAMUK ANOPHELES BARBIROSTRIS DI LABORATORIUM DAN LAPANGAN
| Main Authors: | Widyastuti, Umi, Widiarti, Widiarti, P., Blondine Ch. |
|---|---|
| Other Authors: | BADAN LITBANGKES KEMENKES |
| Format: | application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Badan Penelitian dan Pengembangan Kesehatan
, 2012
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| Subjects: | |
| Online Access: |
http://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/178 |
| ctrlnum |
article-178 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">UJI COBA BACILLUS THURINGIENSIS H-14 TERHADAP JENTIK NYAMUK ANOPHELES BARBIROSTRIS DI LABORATORIUM DAN LAPANGAN</title><creator>Widyastuti, Umi</creator><creator>Widiarti, Widiarti</creator><creator>P., Blondine Ch.</creator><subject lang="id-ID">Health; Kesehatan</subject><subject lang="id-ID">Anopheles barbirostris; Bacillus thuringiensis</subject><subject lang="id-ID"/><description lang="id-ID">A study was conducted in the Vector Control Research Station laboratory in Salatiga and in ponds belongs to inhabitants of Sukutukan village, Wulanggitang Sub District, East Flores Regency. These ponds were found to be an area of breeding sites for Anopheles barbirostris. This study was conducted from August - September 1992 to determine the efficacy of Bacillus thuringiensis H-14 against An. barbirostris larvae in the laboratory and in the field. The effect of B. thuringiensis H-14 (Teknar SC) toward An. barbirostris larvae in the laboratory was conducted according to WHO guidelines to assess the lethal concentrations (LC50 and LC90) which were calculated through the probit analysis. Laboratory tests of B. thuringiensis H-14 (Teknar SC) showed that after 24 hours of exposure, concentrations of 0.9893 and 11.2906 ppm caused approximately 50% and 90% mortality, respectively, for early fourth-instar larvae of An. barbirostris. After 48 hours of exposure, the LC50 and LC90 values calculated for early fourth-instar larvae of An. barbirostris were 0.1499 and 3.3396 ppm, respectively. Test of standard B. thuringiensis H-14 (IPS 82) concentrations required 110 to 522 times lower than Teknar SC, the LC50 and LC90 values for An. barbirostris larvae were estimated as 0.0021 and 0.1024 ppm, respectively, after 24 hours of exposure, while after 48 hours of exposure, the LC50 and LC90 values were 0.0012 and 0.0064 ppm respectively. In the field tests, Teknar SC at dosages of 0.6, 0.75, 1.0 and 1.2 l/Ha caused 84.25%, 84.91%, 91.38% and 84.58% reduction in 1-11 instar An. barbirostris larvae, respectively, while in III-IV instar larvae it was 89.15%, 90.62%, 82.99% and 92.0% respectively after 24 hours exposure. No significant difference in percent reduction between dosages used (p&gt;0.05). Six days after application of Teknar SC, reduction of larval population density were very low with very little or negative reduction.</description><publisher lang="en-US">Badan Penelitian dan Pengembangan Kesehatan</publisher><contributor lang="id-ID">BADAN LITBANGKES KEMENKES</contributor><date>2012-09-05</date><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/178</identifier><source lang="id-ID">Buletin Penelitian Kesehatan; Vol 23, No 1 Mar (1995)</source><source lang="en-US">Buletin Penelitian Kesehatan; Vol 23, No 1 Mar (1995)</source><language>ind</language><rights>The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.</rights><recordID>article-178</recordID></dc>
|
| language |
ind |
| format |
Other: Other File:application/pdf File Journal:eJournal Journal |
| author |
Widyastuti, Umi Widiarti, Widiarti P., Blondine Ch. |
| author2 |
BADAN LITBANGKES KEMENKES |
| title |
UJI COBA BACILLUS THURINGIENSIS H-14 TERHADAP JENTIK NYAMUK ANOPHELES BARBIROSTRIS DI LABORATORIUM DAN LAPANGAN |
| publisher |
Badan Penelitian dan Pengembangan Kesehatan |
| publishDate |
2012 |
| topic |
Health Kesehatan Anopheles barbirostris Bacillus thuringiensis |
| url |
http://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/178 |
| contents |
A study was conducted in the Vector Control Research Station laboratory in Salatiga and in ponds belongs to inhabitants of Sukutukan village, Wulanggitang Sub District, East Flores Regency. These ponds were found to be an area of breeding sites for Anopheles barbirostris. This study was conducted from August - September 1992 to determine the efficacy of Bacillus thuringiensis H-14 against An. barbirostris larvae in the laboratory and in the field. The effect of B. thuringiensis H-14 (Teknar SC) toward An. barbirostris larvae in the laboratory was conducted according to WHO guidelines to assess the lethal concentrations (LC50 and LC90) which were calculated through the probit analysis. Laboratory tests of B. thuringiensis H-14 (Teknar SC) showed that after 24 hours of exposure, concentrations of 0.9893 and 11.2906 ppm caused approximately 50% and 90% mortality, respectively, for early fourth-instar larvae of An. barbirostris. After 48 hours of exposure, the LC50 and LC90 values calculated for early fourth-instar larvae of An. barbirostris were 0.1499 and 3.3396 ppm, respectively. Test of standard B. thuringiensis H-14 (IPS 82) concentrations required 110 to 522 times lower than Teknar SC, the LC50 and LC90 values for An. barbirostris larvae were estimated as 0.0021 and 0.1024 ppm, respectively, after 24 hours of exposure, while after 48 hours of exposure, the LC50 and LC90 values were 0.0012 and 0.0064 ppm respectively. In the field tests, Teknar SC at dosages of 0.6, 0.75, 1.0 and 1.2 l/Ha caused 84.25%, 84.91%, 91.38% and 84.58% reduction in 1-11 instar An. barbirostris larvae, respectively, while in III-IV instar larvae it was 89.15%, 90.62%, 82.99% and 92.0% respectively after 24 hours exposure. No significant difference in percent reduction between dosages used (p>0.05). Six days after application of Teknar SC, reduction of larval population density were very low with very little or negative reduction. |
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