Phycocyanin Extraction from Spirulina platensis and Its Antimalarial Activity In-Vitro
| Main Authors: | Wulandari, Diah Anggraini, Setyaningsih, Iriani, Syafrudin, Din, Setia Asih, Puji Budi |
|---|---|
| Format: | Article info application/pdf |
| Bahasa: | eng |
| Terbitan: |
Department of Aquatic Product Technology
, 2016
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jphpi/article/view/12271 http://journal.ipb.ac.id/index.php/jphpi/article/view/12271/9424 |
| ctrlnum |
article-12271 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Phycocyanin Extraction from Spirulina platensis and Its Antimalarial Activity In-Vitro</title><creator>Wulandari, Diah Anggraini</creator><creator>Setyaningsih, Iriani</creator><creator>Syafrudin, Din</creator><creator>Setia Asih, Puji Budi</creator><subject lang="en-US">antimalarial activity</subject><subject lang="en-US">C-phycocyanin</subject><subject lang="en-US">in-vitro culture</subject><subject lang="en-US">Plasmodium falciparum</subject><subject lang="en-US">Spirulina platensis</subject><description lang="en-US">Phycocyanin is a pigment-protein complex from the light-harvesting phycobiliprotein family which isoften found in cyanobacteria. The product phycocyanin produced by phanizomenon flos-aquae and Spirulinasp. The aim of this study were to determine the best solvents purification phycocanin from Spirulina platensisin three solvents, phosphate buffer, water and aceton ammonium sulphate and to evaluate the antimalarialactivity in vitro of phycocyanin in the best solvent extraction from S. platensis. The method of this study wasusing in-vitro antimalarial method. The result showed C- phycocyanin (C-PC), yield, and protein contentsof phycocyanin were 8 mg/mL, 20.22%, 1.88% extracted and purified by phosphate buffer, 6.63 mg/mL,16.58 %, 3.51% extracted and purified by water, 2.86 mg/mL, 7.15%, 8.4% extracted and purified by acetoneammonium sulphate respectively. Phosphate buffer was the best solvent of phycocyanin extraction from S.platensis. Antimalarial activity in vitro of phycocyanin in hosphate buffer against Plasmodium falciparumstrains 3D7 with IC50 was 158,489 μg/mL. The possible mechanism might be relied on the destruction ofpolymerization of Haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of theplasma membrane.</description><publisher lang="en-US">Department of Aquatic Product Technology</publisher><date>2016-04-25</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/jphpi/article/view/12271</identifier><identifier>10.17844/jphpi.v19i1.12271</identifier><source lang="en-US">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol. 19 No. 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia; 17-25</source><source>2354-886X</source><source>2303-2111</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/jphpi/article/view/12271/9424</relation><rights lang="en-US">Copyright (c) 2016 Jurnal Pengolahan Hasil Perikanan Indonesia</rights><recordID>article-12271</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File |
| author |
Wulandari, Diah Anggraini Setyaningsih, Iriani Syafrudin, Din Setia Asih, Puji Budi |
| title |
Phycocyanin Extraction from Spirulina platensis and Its Antimalarial Activity In-Vitro |
| publisher |
Department of Aquatic Product Technology |
| publishDate |
2016 |
| topic |
antimalarial activity C-phycocyanin in-vitro culture Plasmodium falciparum Spirulina platensis |
| url |
http://journal.ipb.ac.id/index.php/jphpi/article/view/12271 http://journal.ipb.ac.id/index.php/jphpi/article/view/12271/9424 |
| contents |
Phycocyanin is a pigment-protein complex from the light-harvesting phycobiliprotein family which isoften found in cyanobacteria. The product phycocyanin produced by phanizomenon flos-aquae and Spirulinasp. The aim of this study were to determine the best solvents purification phycocanin from Spirulina platensisin three solvents, phosphate buffer, water and aceton ammonium sulphate and to evaluate the antimalarialactivity in vitro of phycocyanin in the best solvent extraction from S. platensis. The method of this study wasusing in-vitro antimalarial method. The result showed C- phycocyanin (C-PC), yield, and protein contentsof phycocyanin were 8 mg/mL, 20.22%, 1.88% extracted and purified by phosphate buffer, 6.63 mg/mL,16.58 %, 3.51% extracted and purified by water, 2.86 mg/mL, 7.15%, 8.4% extracted and purified by acetoneammonium sulphate respectively. Phosphate buffer was the best solvent of phycocyanin extraction from S.platensis. Antimalarial activity in vitro of phycocyanin in hosphate buffer against Plasmodium falciparumstrains 3D7 with IC50 was 158,489 μg/mL. The possible mechanism might be relied on the destruction ofpolymerization of Haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of theplasma membrane. |
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Institut Pertanian Bogor |
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JAWA BARAT |
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