Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis Penyebab Penyakit Darah pada Pisang

Main Authors: Imas, Aisyah, Giyanto, Giyanto, Sinaga, Meity Suradji, Nawangsih, Abdjad Asih, Pari, Gustan
Format: Article info application/pdf Journal
Bahasa: ind
Terbitan: The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia) , 2018
Subjects:
Online Access: https://journal.ipb.ac.id/index.php/jfiti/article/view/20725
https://journal.ipb.ac.id/index.php/jfiti/article/view/20725/15311
ctrlnum article-20725
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis Penyebab Penyakit Darah pada Pisang</title><creator>Imas, Aisyah</creator><creator>Giyanto, Giyanto</creator><creator>Sinaga, Meity Suradji</creator><creator>Nawangsih, Abdjad Asih</creator><creator>Pari, Gustan</creator><subject lang="en-US">antimicrobe</subject><subject lang="en-US">coconut shell</subject><subject lang="en-US">palm oil</subject><subject lang="en-US">pinecone</subject><subject lang="en-US">wood waste</subject><description lang="en-US">In Vitro Test of Liquid Smoke against Ralstonia syzygii&#xA0;subsp.&#xA0;Celebesensis, the Cause of Blood Disease in Bananas&#xA0;Blood disease, caused by Ralstonia syzygii subsp. celebesensis, is an important disease of banana plants in Indonesia. Several control methods have been done, but the result were not effective.&#xA0; Liquid smoke from wood waste is reported to be antimicrobial, but its use for controlling R. syzygii subsp. celebesensis has never been reported. Research was conducted to examine the ability of liquid smoke produced from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) in inhibiting the growth of R. syzygii subsp. celebensensis in vitro and its effect on bacterial cell morphology. Efficacy test of liquid smoke was carried out by agar diffusion method and measurement of bacterial density by spectrophotometry with &#x28E; 600 nm. Observation of bacterial cell morphology was carried out by electron microscopy. The CS-LS, P-LS, and OPB-LS starting from 0.5% concentration showed inhibitory activity against R. syzygii subsp. celebesensis both on triphenyl tetrazolium chloride medium and luria bertani broth. Liquid smoke also caused damage to cell walls and cell membranes. Therefore, liquid smoke has the potential to be used as component in control method for R. syzygii subsp. celebesensis.</description><publisher lang="en-US">The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)</publisher><date>2018-11-22</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>File:application/pdf</type><identifier>https://journal.ipb.ac.id/index.php/jfiti/article/view/20725</identifier><identifier>10.14692/jfi.14.4.145</identifier><source lang="en-US">Jurnal Fitopatologi Indonesia; Vol. 14 No. 4 (2018); 145</source><source lang="id-ID">Jurnal Fitopatologi Indonesia; Vol 14 No 4 (2018); 145</source><source>2339-2479</source><source>0215-7950</source><language>ind</language><relation>https://journal.ipb.ac.id/index.php/jfiti/article/view/20725/15311</relation><rights lang="en-US">Copyright (c) 2018 Jurnal Fitopatologi Indonesia</rights><recordID>article-20725</recordID></dc>
language ind
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
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File:application/pdf
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author Imas, Aisyah
Giyanto, Giyanto
Sinaga, Meity Suradji
Nawangsih, Abdjad Asih
Pari, Gustan
title Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis Penyebab Penyakit Darah pada Pisang
publisher The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
publishDate 2018
topic antimicrobe
coconut shell
palm oil
pinecone
wood waste
url https://journal.ipb.ac.id/index.php/jfiti/article/view/20725
https://journal.ipb.ac.id/index.php/jfiti/article/view/20725/15311
contents In Vitro Test of Liquid Smoke against Ralstonia syzygii subsp. Celebesensis, the Cause of Blood Disease in Bananas Blood disease, caused by Ralstonia syzygii subsp. celebesensis, is an important disease of banana plants in Indonesia. Several control methods have been done, but the result were not effective. Liquid smoke from wood waste is reported to be antimicrobial, but its use for controlling R. syzygii subsp. celebesensis has never been reported. Research was conducted to examine the ability of liquid smoke produced from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) in inhibiting the growth of R. syzygii subsp. celebensensis in vitro and its effect on bacterial cell morphology. Efficacy test of liquid smoke was carried out by agar diffusion method and measurement of bacterial density by spectrophotometry with ʎ 600 nm. Observation of bacterial cell morphology was carried out by electron microscopy. The CS-LS, P-LS, and OPB-LS starting from 0.5% concentration showed inhibitory activity against R. syzygii subsp. celebesensis both on triphenyl tetrazolium chloride medium and luria bertani broth. Liquid smoke also caused damage to cell walls and cell membranes. Therefore, liquid smoke has the potential to be used as component in control method for R. syzygii subsp. celebesensis.
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