Penyakit Mosaik Kuning Pada Kedelai
| Main Authors: | Sutrawati, Mimi, Hidayat, Sri Hendrastuti, Wahyu Soekarno, Bonny Purnomo, Nurmansyah, Ali |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2020
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| Subjects: | |
| Online Access: |
https://journal.ipb.ac.id/index.php/jfiti/article/view/26786 https://journal.ipb.ac.id/index.php/jfiti/article/view/26786/19209 |
| ctrlnum |
article-26786 |
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| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Penyakit Mosaik Kuning Pada Kedelai</title><title lang="id-ID">Yellow mosaic Disease on Soybean</title><creator>Sutrawati, Mimi</creator><creator>Hidayat, Sri Hendrastuti</creator><creator>Wahyu Soekarno, Bonny Purnomo</creator><creator>Nurmansyah, Ali</creator><subject lang="en-US">Mungbean yellow mosaic India virus</subject><subject lang="en-US">phylogenetic analysis</subject><subject lang="en-US">polymerase chain reaction</subject><subject lang="en-US">sequence analysis</subject><subject lang="id-ID">analisis filogenetik</subject><subject lang="id-ID">analisis sikuen</subject><subject lang="id-ID">Mungbean yellow mosaic India virus</subject><subject lang="id-ID">polymerase chain reaction</subject><description lang="en-US">Yellow mosaic Disease on Soybean
&nbsp;
Epidemic of yellow mosaic disease had occurred in yard long bean (Vigna unguiculata subsp. sesquipedalis) in Java in 2008. However, this disease has never been reported in other areas in Indonesia. Observations on soybean plants in Cirebon (West Java), Bantul (DI Yogyakarta), and Musi Banyuasin (South Sumatra) showed systemic symptoms of yellow mosaic on leaves with vein banding. Plant samples showing yellow mosaic symptoms were taken for virus detection using PCR (polymerase chain reaction). The amplification results with specific MYMIV primers showed 1000 bp DNA bands. The results of analysis nucleotide sequence homology showed MYMIV Cirebon has a high homology (98.6-99.2 %) with MYMIV from Tegal, Subang, Klaten, Magelang, Brebes, and Bogor. This confirmed that the yellow mosaic symptom leaf samples from Bantul, Musi Banyuasin, and Cirebon were positively infected with MYMIV. Phylogenetic analysis shows that the Cirebon MYMIV isolates formed a group with all MYMIV sequences in Indonesia and Oman, and separated from MYMIV groups from other countries namely Bangladesh, Nepal, Pakistan and India. MYMIV Cirebon isolate was registered in GenBank with accession number LC481009. MYMIV has been reported in soybean in Java (2013). This finding is the first report of MYMIV infection in soybeans in South Sumatera.</description><description lang="id-ID">Ledakan penyakit mosaik kuning yang disebabkan oleh Mungbean yellow mosaic India Begomovirus (MYMIV) terjadi pada tanaman kacang panjang (Vigna unguiculata subsp. sesquipedalis) di Jawa pada 2008. Namun penyakit ini belum dilaporkan di daerah lainnya di Indonesia. Tanaman kedelai di Cirebon, Jawa Barat, Bantul, DI Yogyakarta, dan Musi Banyuasin Sumatera Selatan menunjukkan gejala sistemik berupa mosaik kuning pada daun dengan tulang daun lebih gelap (vein banding) dan diduga terinfeksi MYMIV. Deteksi dan identifikasi virus dilakukan dengan metode polimerase chain reaction (PCR) dengan menggunakan primer spesifik MYMIV. Hasil amplifikasi menghasilkan pita DNA berukuran 1000 pb. Hasil ini menunjukkan bahwa tanaman kedelai yang bergejala mosaik kuning dari Cirebon, Bantul, Musi Banyuasin, dan positif terinfeksi MYMIV. Hasil analisis homologi sikuen nukleotida tanaman kedelai yang terinfeksi&nbsp; MYMIV asal Cirebon mempunyai homologi yang tinggi (98.6-99.2 %) dengan MYMIV dari Tegal, Subang, Klaten, Magelang, Brebes, dan Bogor yang menginfeksi tanaman kacang panjang. Analisis filogenetik menunjukkan bahwa isolat MYMIV Cirebon berada satu kelompok dengan semua sikuen MYMIV di Indonesia dan Oman, namun terpisah dengan kelompok MYMIV dari&nbsp; Bangladesh, Nepal, Pakistan dan India. Sikuen isolat MYMIV Cirebon telah didaftarkan ke GenBank dengan nomor aksesi LC481009. Sebelumnya MYMIV dilaporkan pada kedelai di Jawa tahun 2013. Temuan ini merupakan laporan pertama infeksi MYMIV pada kedelai di luar Jawa (Musi Banyuasin, Sumatera Selatan).</description><publisher lang="en-US">The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)</publisher><date>2020-01-31</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>File:application/pdf</type><identifier>https://journal.ipb.ac.id/index.php/jfiti/article/view/26786</identifier><identifier>10.14692/jfi.16.1.30-36</identifier><source lang="en-US">Jurnal Fitopatologi Indonesia; Vol. 16 No. 1 (2020); 30-36</source><source lang="id-ID">Jurnal Fitopatologi Indonesia; Vol 16 No 1 (2020); 30-36</source><source>2339-2479</source><source>0215-7950</source><language>ind</language><relation>https://journal.ipb.ac.id/index.php/jfiti/article/view/26786/19209</relation><rights lang="en-US">Copyright (c) 2020 Jurnal Fitopatologi Indonesia</rights><recordID>article-26786</recordID></dc>
|
| language |
ind |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:Journal |
| author |
Sutrawati, Mimi Hidayat, Sri Hendrastuti Wahyu Soekarno, Bonny Purnomo Nurmansyah, Ali |
| title |
Penyakit Mosaik Kuning Pada Kedelai |
| publisher |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia) |
| publishDate |
2020 |
| topic |
Mungbean yellow mosaic India virus phylogenetic analysis polymerase chain reaction sequence analysis analisis filogenetik analisis sikuen |
| url |
https://journal.ipb.ac.id/index.php/jfiti/article/view/26786 https://journal.ipb.ac.id/index.php/jfiti/article/view/26786/19209 |
| contents |
Yellow mosaic Disease on Soybean
Epidemic of yellow mosaic disease had occurred in yard long bean (Vigna unguiculata subsp. sesquipedalis) in Java in 2008. However, this disease has never been reported in other areas in Indonesia. Observations on soybean plants in Cirebon (West Java), Bantul (DI Yogyakarta), and Musi Banyuasin (South Sumatra) showed systemic symptoms of yellow mosaic on leaves with vein banding. Plant samples showing yellow mosaic symptoms were taken for virus detection using PCR (polymerase chain reaction). The amplification results with specific MYMIV primers showed 1000 bp DNA bands. The results of analysis nucleotide sequence homology showed MYMIV Cirebon has a high homology (98.6-99.2 %) with MYMIV from Tegal, Subang, Klaten, Magelang, Brebes, and Bogor. This confirmed that the yellow mosaic symptom leaf samples from Bantul, Musi Banyuasin, and Cirebon were positively infected with MYMIV. Phylogenetic analysis shows that the Cirebon MYMIV isolates formed a group with all MYMIV sequences in Indonesia and Oman, and separated from MYMIV groups from other countries namely Bangladesh, Nepal, Pakistan and India. MYMIV Cirebon isolate was registered in GenBank with accession number LC481009. MYMIV has been reported in soybean in Java (2013). This finding is the first report of MYMIV infection in soybeans in South Sumatera. Ledakan penyakit mosaik kuning yang disebabkan oleh Mungbean yellow mosaic India Begomovirus (MYMIV) terjadi pada tanaman kacang panjang (Vigna unguiculata subsp. sesquipedalis) di Jawa pada 2008. Namun penyakit ini belum dilaporkan di daerah lainnya di Indonesia. Tanaman kedelai di Cirebon, Jawa Barat, Bantul, DI Yogyakarta, dan Musi Banyuasin Sumatera Selatan menunjukkan gejala sistemik berupa mosaik kuning pada daun dengan tulang daun lebih gelap (vein banding) dan diduga terinfeksi MYMIV. Deteksi dan identifikasi virus dilakukan dengan metode polimerase chain reaction (PCR) dengan menggunakan primer spesifik MYMIV. Hasil amplifikasi menghasilkan pita DNA berukuran 1000 pb. Hasil ini menunjukkan bahwa tanaman kedelai yang bergejala mosaik kuning dari Cirebon, Bantul, Musi Banyuasin, dan positif terinfeksi MYMIV. Hasil analisis homologi sikuen nukleotida tanaman kedelai yang terinfeksi MYMIV asal Cirebon mempunyai homologi yang tinggi (98.6-99.2 %) dengan MYMIV dari Tegal, Subang, Klaten, Magelang, Brebes, dan Bogor yang menginfeksi tanaman kacang panjang. Analisis filogenetik menunjukkan bahwa isolat MYMIV Cirebon berada satu kelompok dengan semua sikuen MYMIV di Indonesia dan Oman, namun terpisah dengan kelompok MYMIV dari Bangladesh, Nepal, Pakistan dan India. Sikuen isolat MYMIV Cirebon telah didaftarkan ke GenBank dengan nomor aksesi LC481009. Sebelumnya MYMIV dilaporkan pada kedelai di Jawa tahun 2013. Temuan ini merupakan laporan pertama infeksi MYMIV pada kedelai di luar Jawa (Musi Banyuasin, Sumatera Selatan). |
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