DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH
| Main Authors: | Samarang, Samarang; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI, Satrija, Fadjar; Institut Pertanian Bogor, Murtini, Sri; Institut Pertanian Bogor, Nurjana, Made Agus; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI, Chadijah, Sitti; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI, Maksud, Malonda; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI, Tolistiawaty, Intan; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Badan Penelitian dan Pengembangan Kesehatan
, 2015
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| Subjects: | |
| Online Access: |
http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/4098 |
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article-4098 |
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| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH</title><creator>Samarang, Samarang; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI</creator><creator>Satrija, Fadjar; Institut Pertanian Bogor</creator><creator>Murtini, Sri; Institut Pertanian Bogor</creator><creator>Nurjana, Made Agus; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI</creator><creator>Chadijah, Sitti; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI</creator><creator>Maksud, Malonda; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI</creator><creator>Tolistiawaty, Intan; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI</creator><subject lang="en-US">Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia</subject><description lang="en-US">bstrakDeteksi antigen ekskretori-sekretori Schistosoma japonicum (S.japonicum) dengan metode ELISA pada penderita schistosomiasis dilakukan di Napu Kabupaten Poso selama sembilan bulan, yaitu dari April hingga Desember 2013. Tujuan dari penelitian ini adalah mendapatkan nilai optical density (OD)pada penderita positif schistosomiasis dengan infeksi tinggi, sedang, dan rendah. Menetapkan nilai sensitivitas dan spesifiitas dari konformasi ELISA yang digunakan. Kegiatan dalam penelitian yang dilakukan meliputi kegiatan di lapangan dan kegiatan di laboratorium. Kegiatan di lapangan antara lainsurvei tinja dan survei darah. Kegiatan di labotarorium adalah optimasi ELISA. Hasil penelitian yaitu diperoleh nilai absorbansi pada infeksi rendah berkisar 0.468 ± 0.699 dengan kepadatan telur 1-10 telur/ slide, pada infeksi sedang nilai absorbansinya berkisar 0.700 ± 0.899 dengan kepadatan telur 11-20telur/slide dan untuk infeksi tinggi nilai absorbansinya yaitu 0.900 ± 1.166 dengan kepadatan telur 21-44 telur/slide. Nilai sensitivitas sebesar 74% dan untuk nilai spesifiitasnya sebesar 90%. Kesimpulan dari penelitian ini adalah metode ELISA yang dikembangkan mempunyai nilai sensitivitas dan spesifiitasbaik untuk mendeteksi AgES S. japonicum pada serum penderita schistosomiasis.Kata Kunci: Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia.AbstractDetection of Schistosomajaponicum (S.japonicum) excretory-secretory antigens by ELISA method in human schistosomiasiswas conducted in Poso district Napu valey for nine months, from April to December 2013. The purpose of the study was to get the optical density for the low, medium, and high infection at human schistosomiasis and than to determine the specifiity and sensitivity ELISA conformation. The activities in this study with the laboratory and the fild. The fild activities included stool survey and blood survey. The laboratory activities was optimization of the ELISA method. The results of the study obtained value of sensitivity was 74% and specifiity 90%. Absorbance values ranges from 0699±0468 with density of eggs 1-10 eggs/slide was low infection, the absorbance values was 0.700±0.899 for medium infection the density of eggs 11-20 eggs/slide and high infection the absorbance values were 0.900±1,166 with density of eggs 21-44 eggs/slide. Therefore, it can be concluded of this study that developed ELISA method has good sensitivity and specifiity values for detecting ESAg S.japonicumin human schistosomiasis.Keywords: Schistosomiasis, ELISA, sensitivity, specifiity, Indonesia</description><publisher lang="en-US">Badan Penelitian dan Pengembangan Kesehatan</publisher><contributor lang="en-US"/><date>2015-05-27</date><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/4098</identifier><source lang="id-ID">Media Penelitian dan Pengembangan Kesehatan; Vol 25, No 1 Mar (2015); 65-70</source><source lang="en-US">Media Penelitian dan Pengembangan Kesehatan; Vol 25, No 1 Mar (2015); 65-70</source><language>eng</language><rights>The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Media Penelitian dan Pengembangan Kesehatan (Media of Health Research &amp; Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Media Penelitian dan Pengembangan Kesehatan (Media of Health Research &amp; Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Media Penelitian dan Pengembangan Kesehatan (Media of Health Research &amp; Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.</rights><recordID>article-4098</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal File:application/pdf File Journal:eJournal |
| author |
Samarang, Samarang; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI Satrija, Fadjar; Institut Pertanian Bogor Murtini, Sri; Institut Pertanian Bogor Nurjana, Made Agus; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI Chadijah, Sitti; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI Maksud, Malonda; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI Tolistiawaty, Intan; Balai Litbang P2B2 Donggala, Badan Litbangkes, Kemenkes RI |
| title |
DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH |
| publisher |
Badan Penelitian dan Pengembangan Kesehatan |
| publishDate |
2015 |
| topic |
Schistosomiasis ELISA Sensitivitas Spesifiitas Indonesia |
| url |
http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/4098 |
| contents |
bstrakDeteksi antigen ekskretori-sekretori Schistosoma japonicum (S.japonicum) dengan metode ELISA pada penderita schistosomiasis dilakukan di Napu Kabupaten Poso selama sembilan bulan, yaitu dari April hingga Desember 2013. Tujuan dari penelitian ini adalah mendapatkan nilai optical density (OD)pada penderita positif schistosomiasis dengan infeksi tinggi, sedang, dan rendah. Menetapkan nilai sensitivitas dan spesifiitas dari konformasi ELISA yang digunakan. Kegiatan dalam penelitian yang dilakukan meliputi kegiatan di lapangan dan kegiatan di laboratorium. Kegiatan di lapangan antara lainsurvei tinja dan survei darah. Kegiatan di labotarorium adalah optimasi ELISA. Hasil penelitian yaitu diperoleh nilai absorbansi pada infeksi rendah berkisar 0.468 ± 0.699 dengan kepadatan telur 1-10 telur/ slide, pada infeksi sedang nilai absorbansinya berkisar 0.700 ± 0.899 dengan kepadatan telur 11-20telur/slide dan untuk infeksi tinggi nilai absorbansinya yaitu 0.900 ± 1.166 dengan kepadatan telur 21-44 telur/slide. Nilai sensitivitas sebesar 74% dan untuk nilai spesifiitasnya sebesar 90%. Kesimpulan dari penelitian ini adalah metode ELISA yang dikembangkan mempunyai nilai sensitivitas dan spesifiitasbaik untuk mendeteksi AgES S. japonicum pada serum penderita schistosomiasis.Kata Kunci: Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia.AbstractDetection of Schistosomajaponicum (S.japonicum) excretory-secretory antigens by ELISA method in human schistosomiasiswas conducted in Poso district Napu valey for nine months, from April to December 2013. The purpose of the study was to get the optical density for the low, medium, and high infection at human schistosomiasis and than to determine the specifiity and sensitivity ELISA conformation. The activities in this study with the laboratory and the fild. The fild activities included stool survey and blood survey. The laboratory activities was optimization of the ELISA method. The results of the study obtained value of sensitivity was 74% and specifiity 90%. Absorbance values ranges from 0699±0468 with density of eggs 1-10 eggs/slide was low infection, the absorbance values was 0.700±0.899 for medium infection the density of eggs 11-20 eggs/slide and high infection the absorbance values were 0.900±1,166 with density of eggs 21-44 eggs/slide. Therefore, it can be concluded of this study that developed ELISA method has good sensitivity and specifiity values for detecting ESAg S.japonicumin human schistosomiasis.Keywords: Schistosomiasis, ELISA, sensitivity, specifiity, Indonesia |
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