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The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction

Main Authors: Hamid, Iwan Sahrial, P., Yuseni Kusuma, Bijanti, Retno, Aksono, Bimo
Format: Article info application/pdf Document
Bahasa: eng
Terbitan: Indonesian Society for Cancer Chemoprevention , 2012
Online Access: https://ijcc.chemoprev.org/index.php/ijcc/article/view/62
https://ijcc.chemoprev.org/index.php/ijcc/article/view/62/62
ctrlnum article-62
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction</title><creator>Hamid, Iwan Sahrial</creator><creator>P., Yuseni Kusuma</creator><creator>Bijanti, Retno</creator><creator>Aksono, Bimo</creator><description lang="en-US">Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect of Gynura procumbens (Lour) Merr focusing on the decreasing of the number of macrophages and heterophils on chick embryo chorioallantoic membrane. Nine-days-aged-eggs were divided into six groups (eight eggs each group). Group I (positive control) eggs were induced with bFGF+Tris HCl. Group II (negative control) eggs were treated with DMSO+Tris HCl. Group III (treatment I) eggs were induced with 60 ng bFGF and treated with ethanolic extract of G. procumbens leaves with the dose of 60 &#xB5;g. The following treatment groups, i.e. group IV (treatment II), group V (treatment III), and group VI (treatment IV) were treated with increasing dose of extract, starting from 75 &#xB5;g, 90 &#xB5;g, and the last was 110 &#xB5;g. Eggs were incubated until they reach the age of twelve days to observe macrophages, while to observe heterophils, eggs were incubated until the age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens in decreasing the number of heterophils could not be observed because some blood smears. These analysis suggest that the ethanolic extract of Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens</description><publisher lang="en-US">Indonesian Society for Cancer Chemoprevention</publisher><contributor lang="en-US"/><date>2012-06-30</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/62</identifier><identifier>10.14499/indonesianjcanchemoprev3iss2pp398-403</identifier><source lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 3, No 2 (2012); 398-403</source><source>2355-8989</source><source>2088-0197</source><language>eng</language><relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/62/62</relation><rights lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</rights><recordID>article-62</recordID></dc>
language eng
format Journal:Article
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File:application/pdf
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author Hamid, Iwan Sahrial
P., Yuseni Kusuma
Bijanti, Retno
Aksono, Bimo
title The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction
publisher Indonesian Society for Cancer Chemoprevention
publishDate 2012
url https://ijcc.chemoprev.org/index.php/ijcc/article/view/62
https://ijcc.chemoprev.org/index.php/ijcc/article/view/62/62
contents Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect of Gynura procumbens (Lour) Merr focusing on the decreasing of the number of macrophages and heterophils on chick embryo chorioallantoic membrane. Nine-days-aged-eggs were divided into six groups (eight eggs each group). Group I (positive control) eggs were induced with bFGF+Tris HCl. Group II (negative control) eggs were treated with DMSO+Tris HCl. Group III (treatment I) eggs were induced with 60 ng bFGF and treated with ethanolic extract of G. procumbens leaves with the dose of 60 μg. The following treatment groups, i.e. group IV (treatment II), group V (treatment III), and group VI (treatment IV) were treated with increasing dose of extract, starting from 75 μg, 90 μg, and the last was 110 μg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while to observe heterophils, eggs were incubated until the age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens in decreasing the number of heterophils could not be observed because some blood smears. These analysis suggest that the ethanolic extract of Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens
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