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THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER Muarenesox talabon

Main Authors: Gadi, Dewi Setiyowati, Trilaksani, Wini, Nurhayati, Tati
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University , 2018
Online Access: http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300
http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300/13398
ctrlnum article-19300
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER Muarenesox talabon</title><creator>Gadi, Dewi Setiyowati</creator><creator>Trilaksani, Wini</creator><creator>Nurhayati, Tati</creator><description lang="en-US">By product Muarenesox talabon swim bladders can be used as a raw material for desperately needed in the food, biomedical, pharmaceutical, and cosmeceuticals industries. The aims of the research were to observed the histological and determine chemical characteristics of swim bladder including proximate and amino acids; extraction of acid soluble collagen and determine the characteristics of collagen including proximate, pH, heavy metals, microbial, amino acids, functional groups, molecular weights, and thermal stability. The morphology of cunang swim bladder consists of outer, middle, and inner layers containing collagen fibers; 33.67&#xB1;0.71%wb and protein whichwere dominated by three amino acids that were glycine, proline, and alanine. Pretreatment by 0.1 M NaOH for 8 hours (K1T4) and acid extraction by 0.25 M acetic acid for 72 hours (M1T3) was the best treatment yielding 14.51&#xB1; 0.43% of collagen; having 12.12&#xB1;0.04% wb of moisture; 88.54 &#xB1; 0.08% wb of protein; 1.31&#xB1;0.23% wb of fat; 0.17&#xB1;0.03% wb of ash. Not detected any heavy metals (Pb, Hg, As, Cd). Acidity pH was 4.31 and negative of E. coli and Salmonella. The main amino acids detected were glycine 241.06 mg/g; proline 88.73mg/g; and alanine 86,98 mg/g; FTIR spectra were revealed the presence of triple helix structures; electrophoresis patterns consisted of 136 kDa of mol weight of &#x3B1;1 and 117 kDa of mol weight of &#x3B1;2 were characterisedto be type I collagen; which had Tmax of 195.59&#xBA;C and &#x394;H 7.8113 J/g.&#xA0;Keywords: acid extraction, swim bladder, collagens, thermal stability</description><publisher lang="en-US">Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University</publisher><date>2018-01-02</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300</identifier><identifier>10.29244/jitkt.v9i2.19300</identifier><source lang="en-US">Jurnal Ilmu dan Teknologi Kelautan Tropis; Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis; 665-683</source><source>2620-309X</source><source>2087-9423</source><source>10.29244/jitkt.v9i2</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300/13398</relation><rights lang="en-US">Copyright (c) 2018 Jurnal Ilmu dan Teknologi Kelautan Tropis</rights><recordID>article-19300</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
File:application/pdf
File
Journal:eJournal
author Gadi, Dewi Setiyowati
Trilaksani, Wini
Nurhayati, Tati
title THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER Muarenesox talabon
publisher Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University
publishDate 2018
url http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300
http://journal.ipb.ac.id/index.php/jurnalikt/article/view/19300/13398
contents By product Muarenesox talabon swim bladders can be used as a raw material for desperately needed in the food, biomedical, pharmaceutical, and cosmeceuticals industries. The aims of the research were to observed the histological and determine chemical characteristics of swim bladder including proximate and amino acids; extraction of acid soluble collagen and determine the characteristics of collagen including proximate, pH, heavy metals, microbial, amino acids, functional groups, molecular weights, and thermal stability. The morphology of cunang swim bladder consists of outer, middle, and inner layers containing collagen fibers; 33.67±0.71%wb and protein whichwere dominated by three amino acids that were glycine, proline, and alanine. Pretreatment by 0.1 M NaOH for 8 hours (K1T4) and acid extraction by 0.25 M acetic acid for 72 hours (M1T3) was the best treatment yielding 14.51± 0.43% of collagen; having 12.12±0.04% wb of moisture; 88.54 ± 0.08% wb of protein; 1.31±0.23% wb of fat; 0.17±0.03% wb of ash. Not detected any heavy metals (Pb, Hg, As, Cd). Acidity pH was 4.31 and negative of E. coli and Salmonella. The main amino acids detected were glycine 241.06 mg/g; proline 88.73mg/g; and alanine 86,98 mg/g; FTIR spectra were revealed the presence of triple helix structures; electrophoresis patterns consisted of 136 kDa of mol weight of α1 and 117 kDa of mol weight of α2 were characterisedto be type I collagen; which had Tmax of 195.59oC and ΔH 7.8113 J/g. Keywords: acid extraction, swim bladder, collagens, thermal stability
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