Evaluasi Histopatologis Cedera Remote Iskemik Reperfusi Organ Hepar Kelinci Yang Dilakukan Prekondisi Iskemik Dan Hipotermia = Histopatologic Evaluation On Remote Ischemic Reperfusion Injury Of Rabbit Liver Performed Under Ischemic Precondiotioning And Hypothermia
| Main Author: | Marolop Pardede |
|---|---|
| Format: | Book xvi, 43 hlm, 21 x 29 cm |
| Bahasa: | ind |
| Terbitan: |
Program Studi Ilmu Bedah Toraks Kardiovaskular
, 2014
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| Subjects: | |
| Online Access: |
http://perpustakaan.fk.ui.ac.id/new-opac/index.php?p=show_detail&id=19103 |
Daftar Isi:
- Latar Belakang: Cedera iskemik reperfusi merupakan suatu kondisi yg seringdijumpai, dan dapat menyebabkan gangguan secara sitemik pada organ tubuh.Antara lain dapat menyebabkan kerusakan ke jaringan hepar secara remote. Tujuan: Mengetahui manfaat perlakuan prekondisi iskemik dan hipotermia padacedera iskemik reperfusi tungkai bawah guna mencegah terjadinya kerusakanpada jaringan hepar Metode. Dilakukan penelitian eksperimental pada kelinci New Zealand White(n=18) dengan satu kelompok kontrol (iskemia) dan dua kelompok perlakuan(hipotermia dan prekondisi iskemik). Dilakukan ligasi a. iliaca communisselama 4 jam, hipotermia (28oC), dan prekondisi iskemia pada masing-masingkelompok. Kemudian ligasi dilepas untuk reperfusi selama 8 jam. Lalu kelinci dieuthanasia, jaringan hepar diambil untuk pemeriksaan histopatologi (Skoringpenelitian).Hasil: Terdapat kerusakan jaringan hepar yang dinilai secara histopatologisebagai efek cedera iskemik reperfusi jauh yang di akibatkan oleh iskemiktungkai akut pada hewan coba kelinci. Dengan melakukan skoring terhadap selhepatosit, sitoplasma, sinusoid, batas interselular, mikrohemoragik, dan infiltrasileukosit. Skor kelompok kontrol dengan median 89,50 min-max 75-91,Kelompok perlakuan IPC mean 49,17, SD 15,53. Kelompok perlakuanhipotermia mean 42,83 SD 22,02. Prekondisi iskemik dan hipotermia dapatmengurangi terjadi kerusakan jauh pada hepar secara bermakna dengan nilaip=0,002 dan p=0,004Simpulan: Terbukti terjadinya kerusakan remote iskemik reperfusi pada heparakibat cedera iskemik reperfusi tungkai akut, Hipotermia dan iskemik prekondisisecara signifikan dapat mengurangi terjadinya kerusakan itu.Background: ischemic reperfusion injury is a common condition that may disruptsystemic organs, especially causing liver damage remotely.Objectives: to evaluate the benefit of ischemic preconditioning and hypothermiaon ischemic reperfusion injury of the lower limbs from liver tissue damage.Methods: this experimental study was performed using New Zealand WhiteRabbits (n=18) that were grouped into control group (ischemia) and treatmentgroup (ischemic preconditioning and hypothermia). Iliaca communis artery wasligated for 4 hours and treated group had topical cooling until 28C andperformed ischmeic preconditioning. The ligation was released for reperfusion for 8 hours. The rabbits were then euthanized and liver tissue was taken for histopathologic examination.Result: liver tissue damage was found histopathologically caused by remoteischemic reperfusion of rabbit limbs. By performing scoring of hepatocyte cells,cytoplasm, sinusioids, intercellular cells, microhemorrhage, and leucocyteinfiltration. The median score for control group was 89.50 (ranged 75-91), and49.17 for IPC group (SD 15.53).mean score for hypothermia group is 42.83 (SD22.02). IPC and hypothermia showed to reduce liver damage significantly (p =0.002 and p = 0.004).Conclusion: it has proven that liver damage occurs in remote ischemicreperfusion injury of limbs and iscemic preconditioning and hypothermia havereduce the damage.