Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment

Main Authors: Trivedi, Mahendra Kumar, Branton, Alice, Trivedi, Dahryn, Nayak, Gopal, Gangwar, Mayank, Jana, Snehasis
Format: Article Journal
Bahasa: eng
Terbitan: , 2018
Subjects:
Online Access: https://zenodo.org/record/2564484
ctrlnum 2564484
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><creator>Trivedi, Mahendra Kumar</creator><creator>Branton, Alice</creator><creator>Trivedi, Dahryn</creator><creator>Nayak, Gopal</creator><creator>Gangwar, Mayank</creator><creator>Jana, Snehasis</creator><date>2018-01-01</date><description>Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect&#xAE;) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p&#x2264;0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect&#xAE; might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line. https://www.trivedieffect.com/science/estimation-of-collagen-synthesis-in-human-dermal-fibroblast-cells-an-impact-of-consciousness-energy-healing-treatment</description><description>Mahendra Kumar Trivedi et.al, "Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment". International Journal of Research Studies in Medical and Health Sciences. 2018; 3(9):18-24.</description><identifier>https://zenodo.org/record/2564484</identifier><identifier>10.5281/zenodo.2564484</identifier><identifier>oai:zenodo.org:2564484</identifier><language>eng</language><relation>doi:10.5281/zenodo.2564483</relation><rights>info:eu-repo/semantics/openAccess</rights><rights>https://creativecommons.org/licenses/by/4.0/legalcode</rights><source>International Journal of Research Studies in Medical and Health Sciences 3(9) 18-24</source><subject>HFF-1 cell line</subject><subject>Consciousness Energy Healing Treatment</subject><subject>Biofield Energy Healing</subject><subject>The Trivedi Effect&#xAE;</subject><subject>Fibroblast cells</subject><subject>Collagen</subject><title>Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment</title><type>Journal:Article</type><type>Journal:Article</type><recordID>2564484</recordID></dc>
language eng
format Journal:Article
Journal
Journal:Journal
author Trivedi, Mahendra Kumar
Branton, Alice
Trivedi, Dahryn
Nayak, Gopal
Gangwar, Mayank
Jana, Snehasis
title Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment
publishDate 2018
topic HFF-1 cell line
Consciousness Energy Healing Treatment
Biofield Energy Healing
The Trivedi Effect®
Fibroblast cells
Collagen
url https://zenodo.org/record/2564484
contents Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p≤0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line. https://www.trivedieffect.com/science/estimation-of-collagen-synthesis-in-human-dermal-fibroblast-cells-an-impact-of-consciousness-energy-healing-treatment
Mahendra Kumar Trivedi et.al, "Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment". International Journal of Research Studies in Medical and Health Sciences. 2018; 3(9):18-24.
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