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author Imachi, Hiroyuki
Nobu, Masaru K.
Nakahara, Nozomi
Morono, Yuki
Ogawara, Miyuki
Takaki, Yoshihiro
Takano, Yoshinori
Uematsu, Katsuyuki
Ikuta, Tetsuro
Ito, Motoo
Matsui, Yohei
Miyazaki, Masayuki
Murata, Kazuyoshi
Saito, Yumi
Sakai, Sanae
Song, Chihong
Tasumi, Eiji
Yamanaka, Yuko
Yamaguchi, Takashi
Kamagata, Yoichi
Tamaki, Hideyuki
Takai, Ken
title Extended Data Fig. 4 in Isolation of an archaeon at the prokaryote eukaryote interface
publishDate 2020
topic Biodiversity
Taxonomy
url https://zenodo.org/record/3609924
contents Extended Data Fig. 4 | Ribosomal protein- and 16S rRNA gene-based phylogeny of MK-D1. a, Phylogenomic tree of MK-D1 and select cultured archaea,eukaryotes and bacteria based on 31 ribosomal proteins conserved across the three domains (Supplementary Table 7). Ribosomal protein sequences of MK-D1,the organisms shown in the tree and MAGs of uncultured archaeal lineages (Supplementary Table 8) were aligned individually using MAFFT.MAG-derived sequences (except for Ca. Korarchaeum) were then removed for tree construction.After removing all-gap positions and concatenation,the maximum-likelihood tree was constructed using RAxML- NG.Bootstrap values around critical branching points are also shown.In total, 14,875 sites of the alignment were used for tree construction. b, A ribosomal protein-based phylogenomic tree constructed using MrBayes.Bayesian inference phylogenies were calculated using MrBayes 3.2.7a and a ribosomal protein concatenated alignment used for Fig.4a. c, Phylogenetic tree of MK-D1 and related archaea based on 16S rRNA genes.The 16S rRNA gene sequences were aligned using SINA against the Silva v.132 alignment and the maximumlikelihood tree was calculated using RAxML.
Published as part of Imachi, Hiroyuki, Nobu, Masaru K., Nakahara, Nozomi, Morono, Yuki, Ogawara, Miyuki, Takaki, Yoshihiro, Takano, Yoshinori, Uematsu, Katsuyuki, Ikuta, Tetsuro, Ito, Motoo, Matsui, Yohei, Miyazaki, Masayuki, Murata, Kazuyoshi, Saito, Yumi, Sakai, Sanae, Song, Chihong, Tasumi, Eiji, Yamanaka, Yuko, Yamaguchi, Takashi, Kamagata, Yoichi, Tamaki, Hideyuki & Takai, Ken, 2020, Nature 41586 on pages 1-23, DOI: 10.1038/s41586-019-1916-6, http://zenodo.org/record/3609900
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