Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)
| Main Authors: | Zhou, Jian, Wang, Shaojing, Yu, Li'ang, Li, Ning, Li, Shufen, Zhang, Yulan, Qin, Ruiyun, Gao, Wujun, Deng, Chuanliang |
|---|---|
| Format: | Article Journal |
| Terbitan: |
Pensoft Publishers
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4731269 |
| ctrlnum |
4731269 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><creator>Zhou, Jian</creator><creator>Wang, Shaojing</creator><creator>Yu, Li'ang</creator><creator>Li, Ning</creator><creator>Li, Shufen</creator><creator>Zhang, Yulan</creator><creator>Qin, Ruiyun</creator><creator>Gao, Wujun</creator><creator>Deng, Chuanliang</creator><date>2021-04-23</date><description>Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomic subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR amplification products of micro-dissected spinach Y chromosome. The screening yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA sequences, four DNA sequences that contained a fragment of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence probes to hybridization on male and female spinach karyotypes. Fluorescence in situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the centromeres and their surrounding area for each homolog. Hybridization signals primarily appeared near the putative centromeres for each homologous chromosome pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the long arms. Results can be served as a basis to study the function of repetitive DNA sequences in sex chromosome evolution in spinach.</description><identifier>https://zenodo.org/record/4731269</identifier><identifier>10.3897/CompCytogen.v15i2.63061</identifier><identifier>oai:zenodo.org:4731269</identifier><publisher>Pensoft Publishers</publisher><relation>doi:10.3897/CompCytogen.v15i2.63061.suppl1</relation><relation>url:https://zenodo.org/communities/biosyslit</relation><rights>info:eu-repo/semantics/openAccess</rights><rights>https://creativecommons.org/licenses/by/4.0/legalcode</rights><source>Comparative Cytogenetics 15((2)) 101-118</source><subject>Plantae</subject><subject>Tracheophyta</subject><subject>Magnoliopsida</subject><subject>Caryophyllales</subject><subject>Amaranthaceae</subject><subject>Spinacia</subject><subject>Spinacia oleracea</subject><subject>FISH</subject><subject>Genomic subtraction hybridization</subject><subject>Retrotransposon</subject><subject>Sex chromosome evolution</subject><subject>Spinach</subject><title>Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)</title><type>Journal:Article</type><type>Journal:Article</type><recordID>4731269</recordID></dc>
|
| format |
Journal:Article Journal Journal:Journal |
| author |
Zhou, Jian Wang, Shaojing Yu, Li'ang Li, Ning Li, Shufen Zhang, Yulan Qin, Ruiyun Gao, Wujun Deng, Chuanliang |
| title |
Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae) |
| publisher |
Pensoft Publishers |
| publishDate |
2021 |
| topic |
Plantae Tracheophyta Magnoliopsida Caryophyllales Amaranthaceae Spinacia Spinacia oleracea FISH Genomic subtraction hybridization Retrotransposon Sex chromosome evolution Spinach |
| url |
https://zenodo.org/record/4731269 |
| contents |
Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomic subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR amplification products of micro-dissected spinach Y chromosome. The screening yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA sequences, four DNA sequences that contained a fragment of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence probes to hybridization on male and female spinach karyotypes. Fluorescence in situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the centromeres and their surrounding area for each homolog. Hybridization signals primarily appeared near the putative centromeres for each homologous chromosome pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the long arms. Results can be served as a basis to study the function of repetitive DNA sequences in sex chromosome evolution in spinach. |
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IOS16997.4731269 |
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ZAIN Publications |
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7213 |
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library:special library |
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Cognizance Journal of Multidisciplinary Studies |
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5267 |
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Cognizance Journal of Multidisciplinary Studies |
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16997 |
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Multidisciplinary |
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Stockholm |
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INTERNASIONAL |
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1 |
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IOS16997 |
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2022-06-06T03:16:20Z |
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2022-06-06T03:16:20Z |
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1734897936320430080 |
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17.610468 |