CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors
| Main Authors: | Uranga, Mireia, Vazquez-Vilar, Marta, Orzáez, Diego, Darós, José-Antonio |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2021
|
| Online Access: |
https://zenodo.org/record/5932122 |
| ctrlnum |
5932122 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><creator>Uranga, Mireia</creator><creator>Vazquez-Vilar, Marta</creator><creator>Orzáez, Diego</creator><creator>Darós, José-Antonio</creator><date>2021-10-15</date><description>The use of viral vectors that can replicate and move systemically through the host plant to deliver bacterialCRISPR components enables genome editing at the whole-plant level and avoids the requirement for labor-intensive stable transformation. However, this approach usually relies on previously transformed plants thatstably express a CRISPR-Cas nuclease. Here, we describe successful DNA-free genome editing ofNicotiana ben-thamianausing two compatible RNA virus vectors derived from tobacco etch virus (TEV; genusPotyvirus) andpotato virus X (PVX; genusPotexvirus), which replicate in the same cells. The TEV and PVX vectors respectivelyexpress a Cas12a nuclease and the corresponding guide RNA. This novel two-virus vector system improvesthe toolbox for transformation-free virus-induced genome editing in plants and will advance efforts to breedmore nutritious, resistant, and productive crops.</description><description>This research was supported by grants BIO2017-83184-Rand PID2019-108203RB-I00 from the Ministerio deCiencia e Innovacio ́n (Spain) through the Agencia Estatalde Investigacio ́n (co-financed by the European RegionalDevelopment Fund) and H2020-760331 Newcotiana fromthe European Commission. M.U. and M.V.-V. are the recip-ients of fellowships FPU17/05503 from the Ministerio deCiencia e Innovacio ́n (Spain) and APOSTD/2020/096from the Generalitat Valenciana (Spain), respectively.</description><identifier>https://zenodo.org/record/5932122</identifier><identifier>10.1089/crispr.2021.0049</identifier><identifier>oai:zenodo.org:5932122</identifier><language>eng</language><relation>info:eu-repo/grantAgreement/EC/H2020/760331/</relation><relation>url:https://zenodo.org/communities/newcotiana</relation><rights>info:eu-repo/semantics/openAccess</rights><rights>https://creativecommons.org/licenses/by-nc/4.0/legalcode</rights><source>The CRISPR Journal 4(5) 761-769</source><title>CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors</title><type>Journal:Article</type><type>Journal:Article</type><recordID>5932122</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Journal:Journal |
| author |
Uranga, Mireia Vazquez-Vilar, Marta Orzáez, Diego Darós, José-Antonio |
| title |
CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors |
| publishDate |
2021 |
| url |
https://zenodo.org/record/5932122 |
| contents |
The use of viral vectors that can replicate and move systemically through the host plant to deliver bacterialCRISPR components enables genome editing at the whole-plant level and avoids the requirement for labor-intensive stable transformation. However, this approach usually relies on previously transformed plants thatstably express a CRISPR-Cas nuclease. Here, we describe successful DNA-free genome editing ofNicotiana ben-thamianausing two compatible RNA virus vectors derived from tobacco etch virus (TEV; genusPotyvirus) andpotato virus X (PVX; genusPotexvirus), which replicate in the same cells. The TEV and PVX vectors respectivelyexpress a Cas12a nuclease and the corresponding guide RNA. This novel two-virus vector system improvesthe toolbox for transformation-free virus-induced genome editing in plants and will advance efforts to breedmore nutritious, resistant, and productive crops. This research was supported by grants BIO2017-83184-Rand PID2019-108203RB-I00 from the Ministerio deCiencia e Innovacio ́n (Spain) through the Agencia Estatalde Investigacio ́n (co-financed by the European RegionalDevelopment Fund) and H2020-760331 Newcotiana fromthe European Commission. M.U. and M.V.-V. are the recip-ients of fellowships FPU17/05503 from the Ministerio deCiencia e Innovacio ́n (Spain) and APOSTD/2020/096from the Generalitat Valenciana (Spain), respectively. |
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ZAIN Publications |
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7213 |
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Cognizance Journal of Multidisciplinary Studies |
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5267 |
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Cognizance Journal of Multidisciplinary Studies |
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16997 |
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Multidisciplinary |
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Stockholm |
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INTERNASIONAL |
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