Alternative splicing and genetic variation of MHC-E: Implications for rhesus cytomegalovirus-based vaccines
Main Authors: | Brochu, Hayden, Wang, Ruihan, Tollison, Tammy, Pyo, Chul-Woo, Thomas, Alexander, Tseng, Elizabeth, Law, Lynn, Gale, Michael, Geraghty, Daniel, Peng, Xinxia |
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Format: | info dataset Journal |
Terbitan: |
, 2022
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Online Access: |
https://zenodo.org/record/5985423 |
ctrlnum |
5985423 |
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fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><creator>Brochu, Hayden</creator><creator>Wang, Ruihan</creator><creator>Tollison, Tammy</creator><creator>Pyo, Chul-Woo</creator><creator>Thomas, Alexander</creator><creator>Tseng, Elizabeth</creator><creator>Law, Lynn</creator><creator>Gale, Michael</creator><creator>Geraghty, Daniel</creator><creator>Peng, Xinxia</creator><date>2022-02-20</date><description>We used long-read sequencing to interrogate rhesus macaque (RM) MHC-E (Mamu-E) alternative splicing and genetic variations. Full-length Mamu-E RNA isoforms were recovered using the PacBio Iso-Seq method. Incomplete 5' ends of Mamu-E isoforms were confirmed using Sanger sequencing, where we identified three additional isoforms. Full-length human MHC-E (HLA-E) isoforms were also recovered using the PacBio Iso-Seq method. Isoform sequences and annotations are provided for both Mamu-E and HLA-E in addition to Mamu-E Sanger sequencing data. HLA-E annotations are reported using the hg38 reference, while Mamu-E annotations are shown using rhesus MHC Class I and II assemblies previously generated using Bacterial Artificial Cloning (BAC) technology (https://www.ncbi.nlm.nih.gov/nuccore/AC148696.1).
Using PacBio Long Amplicon Analysis, we sequenced complete Mamu-E coding regions of 59 RMs and additionally captured 3' UTR polymorphism using mRNA-seq haplotype phasing analysis. The complete genotyping data for these animals are provided as well as animal metadata. Genotyping data is shown using the Mamu-E canonical isoform (Mamu-E1 from Iso-Seq analysis) as reference.</description><identifier>https://zenodo.org/record/5985423</identifier><identifier>10.5281/zenodo.5985423</identifier><identifier>oai:zenodo.org:5985423</identifier><relation>doi:10.5281/zenodo.5985422</relation><rights>info:eu-repo/semantics/restrictedAccess</rights><title>Alternative splicing and genetic variation of MHC-E: Implications for rhesus cytomegalovirus-based vaccines</title><type>Other:info:eu-repo/semantics/other</type><type>Other:dataset</type><recordID>5985423</recordID></dc>
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format |
Other:info:eu-repo/semantics/other Other Other:dataset Journal:Journal Journal |
author |
Brochu, Hayden Wang, Ruihan Tollison, Tammy Pyo, Chul-Woo Thomas, Alexander Tseng, Elizabeth Law, Lynn Gale, Michael Geraghty, Daniel Peng, Xinxia |
title |
Alternative splicing and genetic variation of MHC-E: Implications for rhesus cytomegalovirus-based vaccines |
publishDate |
2022 |
url |
https://zenodo.org/record/5985423 |
contents |
We used long-read sequencing to interrogate rhesus macaque (RM) MHC-E (Mamu-E) alternative splicing and genetic variations. Full-length Mamu-E RNA isoforms were recovered using the PacBio Iso-Seq method. Incomplete 5' ends of Mamu-E isoforms were confirmed using Sanger sequencing, where we identified three additional isoforms. Full-length human MHC-E (HLA-E) isoforms were also recovered using the PacBio Iso-Seq method. Isoform sequences and annotations are provided for both Mamu-E and HLA-E in addition to Mamu-E Sanger sequencing data. HLA-E annotations are reported using the hg38 reference, while Mamu-E annotations are shown using rhesus MHC Class I and II assemblies previously generated using Bacterial Artificial Cloning (BAC) technology (https://www.ncbi.nlm.nih.gov/nuccore/AC148696.1).
Using PacBio Long Amplicon Analysis, we sequenced complete Mamu-E coding regions of 59 RMs and additionally captured 3' UTR polymorphism using mRNA-seq haplotype phasing analysis. The complete genotyping data for these animals are provided as well as animal metadata. Genotyping data is shown using the Mamu-E canonical isoform (Mamu-E1 from Iso-Seq analysis) as reference. |
id |
IOS16997.5985423 |
institution |
ZAIN Publications |
institution_id |
7213 |
institution_type |
library:special library |
library |
Cognizance Journal of Multidisciplinary Studies |
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5267 |
collection |
Cognizance Journal of Multidisciplinary Studies |
repository_id |
16997 |
subject_area |
Multidisciplinary |
city |
Stockholm |
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INTERNASIONAL |
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1 |
repoId |
IOS16997 |
first_indexed |
2022-06-06T04:53:43Z |
last_indexed |
2022-06-06T04:53:43Z |
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17.610611 |