Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa
| Main Authors: | Aprilyani, Aprilyani; Institut Pertanian Bogor, Supramana, Supramana; Institut Pertanian Bogor, Suastika, Gede; Institut Pertanian Bogor |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2016
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jfiti/article/view/10561 http://journal.ipb.ac.id/index.php/jfiti/article/view/10561/8426 |
| ctrlnum |
article-10561 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa</title><creator>Aprilyani, Aprilyani; Institut Pertanian Bogor</creator><creator>Supramana, Supramana; Institut Pertanian Bogor</creator><creator>Suastika, Gede; Institut Pertanian Bogor</creator><subject lang="en-US">detection, phylogenetic analysis, polymerase chain</subject><description lang="en-US">Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia. </description><publisher lang="en-US">The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)</publisher><contributor lang="en-US"/><date>2016-01-12</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/jfiti/article/view/10561</identifier><identifier>10.14692/jfi.11.5.143</identifier><source lang="id-ID">Jurnal Fitopatologi Indonesia; Vol 11, No 5 (2015); 143</source><source lang="en-US">Jurnal Fitopatologi Indonesia; Vol 11, No 5 (2015); 143</source><source>2339-2479</source><source>0215-7950</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/jfiti/article/view/10561/8426</relation><rights lang="en-US">Copyright (c) 2015 Jurnal Fitopatologi Indonesia</rights><recordID>article-10561</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Aprilyani, Aprilyani; Institut Pertanian Bogor Supramana, Supramana; Institut Pertanian Bogor Suastika, Gede; Institut Pertanian Bogor |
| title |
Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa |
| publisher |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia) |
| publishDate |
2016 |
| topic |
detection phylogenetic analysis polymerase chain |
| url |
http://journal.ipb.ac.id/index.php/jfiti/article/view/10561 http://journal.ipb.ac.id/index.php/jfiti/article/view/10561/8426 |
| contents |
Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia. |
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Institut Pertanian Bogor |
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