Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia
| Main Authors: | Yohandini, Heni; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662, Julinar, .; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662, Muharni, .; Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662 |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Bogor Agricultural University, Indonesia
, 2015
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/hayati/article/view/10776 http://journal.ipb.ac.id/index.php/hayati/article/view/10776/8322 |
| ctrlnum |
article-10776 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia</title><creator>Yohandini, Heni; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662</creator><creator>Julinar, .; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662</creator><creator>Muharni, .; Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662</creator><subject lang="en-US">16S rRNA; phylogenetic analysis; Tanjung Sakti Hot Spring; thermophile</subject><description lang="en-US">A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80C-91C and pH 7-8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank.</description><publisher lang="en-US">Bogor Agricultural University, Indonesia</publisher><contributor lang="en-US"/><date>2015-12-30</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/hayati/article/view/10776</identifier><source lang="en-US">HAYATI Journal of Biosciences; Vol 22, No 3 (2015): July 2015; 143-148</source><source>2086-4094</source><source>1978-3019</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/hayati/article/view/10776/8322</relation><rights lang="en-US">Copyright (c) 2015 Heni Yohandini, . Julinar, . Muharni</rights><rights lang="en-US">http://creativecommons.org/licenses/by/4.0</rights><recordID>article-10776</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Yohandini, Heni; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662 Julinar, .; Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662 Muharni, .; Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662 |
| title |
Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| publisher |
Bogor Agricultural University, Indonesia |
| publishDate |
2015 |
| topic |
16S rRNA phylogenetic analysis Tanjung Sakti Hot Spring thermophile |
| url |
http://journal.ipb.ac.id/index.php/hayati/article/view/10776 http://journal.ipb.ac.id/index.php/hayati/article/view/10776/8322 |
| contents |
A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80C-91C and pH 7-8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank. |
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