Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase
| Main Author: | ARTIKA, I MADE; Bogor Agricultural University |
|---|---|
| Other Authors: | Bogor Agricultural University, Indonesia |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Bogor Agricultural University, Indonesia
, 2010
|
| Online Access: |
http://journal.ipb.ac.id/index.php/hayati/article/view/1693 http://journal.ipb.ac.id/index.php/hayati/article/view/1693/736 |
| ctrlnum |
article-1693 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase</title><creator>ARTIKA, I MADE; Bogor Agricultural University</creator><description lang="en-US">The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to subunit 8 protein potentially facilitate elucidation of its topology, structure, and function. It has been shown that following incorporation of FLAG tag to its C-terminus, subunit 8 still assemble into functional ATP synthase complex. In order to analyze bioenergetic consequences of the FLAG tag addition, a yeast strain expressing FLAG tagged-subunit 8 was subjected to cellular respiration assays. Results obtained showed that addition of FLAG tag to the C-terminus of subunit 8 does not impair its proper functioning. The FLAG tag system, therefore, can be employed to study subunit 8’s detailed structure, topology, and function.</description><publisher lang="en-US">Bogor Agricultural University, Indonesia</publisher><contributor lang="en-US">Bogor Agricultural University, Indonesia</contributor><date>2010-09-01</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/hayati/article/view/1693</identifier><source lang="en-US">HAYATI Journal of Biosciences; Vol 17, No 3 (2010): September 2010; 151</source><source>2086-4094</source><source>1978-3019</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/hayati/article/view/1693/736</relation><recordID>article-1693</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
ARTIKA, I MADE; Bogor Agricultural University |
| author2 |
Bogor Agricultural University, Indonesia |
| title |
Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase |
| publisher |
Bogor Agricultural University, Indonesia |
| publishDate |
2010 |
| url |
http://journal.ipb.ac.id/index.php/hayati/article/view/1693 http://journal.ipb.ac.id/index.php/hayati/article/view/1693/736 |
| contents |
The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to subunit 8 protein potentially facilitate elucidation of its topology, structure, and function. It has been shown that following incorporation of FLAG tag to its C-terminus, subunit 8 still assemble into functional ATP synthase complex. In order to analyze bioenergetic consequences of the FLAG tag addition, a yeast strain expressing FLAG tagged-subunit 8 was subjected to cellular respiration assays. Results obtained showed that addition of FLAG tag to the C-terminus of subunit 8 does not impair its proper functioning. The FLAG tag system, therefore, can be employed to study subunit 8’s detailed structure, topology, and function. |
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Institut Pertanian Bogor |
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Perpustakaan Institut Pertanian Bogor |
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Jurnal Matematika dan Aplikasinya |
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JAWA BARAT |
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