Pengaruh antibodi antisperma dari serum wanita infertil terhadap integritas membran plasma, fosforilasi tirosin, viabilitas dan motilitas spermatozoa manusia = Effects of antisperm antibody from infertile woman sera on plasma membrane integrity tyrosine phosphorylation viability and motility of human sperm
| Main Author: | Hajizah, author |
|---|---|
| Format: | Masters Bachelors |
| Terbitan: |
[, Fakultas Kedokteran Universitas Indonesia]
, 2014
|
| Subjects: | |
| Online Access: |
http://lib.ui.ac.id/file?file=digital/2015-12/20404106-T-Hajizah.pdf |
| ctrlnum |
20404106 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Pengaruh antibodi antisperma dari serum wanita infertil terhadap integritas membran plasma, fosforilasi tirosin, viabilitas dan motilitas spermatozoa manusia = Effects of antisperm antibody from infertile woman sera on plasma membrane integrity tyrosine phosphorylation viability and motility of human sperm</title><creator/><creator>Hajizah, author</creator><type>Thesis:Masters</type><place/><publisher>[, Fakultas Kedokteran Universitas Indonesia]</publisher><date>2014</date><description>[<b>ABSTRAK</b><br>
Salah satu faktor yang menentukan keberhasilan proses fertilisasi adalah
kemampuan sperma membuahi oosit. Sperma yang diejakulasikan dari saluran
reproduksi pria belum memiliki kemampuan ini. Di dalam saluran reproduksi
wanita (servik) sperma mengalami serangkaian proses yang membuatnya
memiliki kemampuan membuahi oosit yang disebut kapasitasi. Kapasitasi
merupakan tahapan penting bagi keberhasilan fertilisasi yang memerlukan
integritas membran plasma baik dan dikarakterisasi oleh fosforilasi tirosin.
Sementara itu, bagi tubuh perempuan maupun laki-laki spermatozoa merupakan
benda asing yang dapat merangsang respon imun berupa pembentukan antibodi
terhadap sperma (ASA). Penelitian ini bertujuan untuk mengetahui pengaruh
antibodi antisperma dari serum wanita infertil terhadap integritas membran
plasma, status dan lokalisasi fosforilasi tirosin, serta viabilitas dan motilitas
spermatozoa manusia. Desain penelitian ini adalah eksperimental in vitro di mana
spermatozoa normal donor fertil yang telah dicuci diberi perlakuan diinkubasi
dengan serum yang terdeteksi ASA dari wanita infertil dengan kelompok
konsentrasi serum 0, 1/1000, 1/100 dan 1/10 selama 1 jam dan 2 jam, kemudian
diperiksa parameter-parameter; integritas membran plasma, intensitas dan lokasi
fosforilasi tirosin serta viabilitas dan motilitas spermatozoa. Hasil pemeriksaan
untuk semua parameter yang diukur menunjukkan kecenderungan yang sama,
yaitu inkubasi spermatozoa dengan serum positif ASA dengan konsentrasi
meningkat menyebabkan penurunan persentase integritas membran plasma,
fosforilasi tirosin, viabilitas dan motilitas spermatozoa untuk waktu inkubasi 1
jam, namun tidak demikian untuk inkubasi selama 2 jam. Namun secara
keseluruhan semua parameter menunjukkan nilai persentase yang lebih rendah
untuk inkubasi selama 2 jam dibandingkan 1 jam. Dengan demikian pada
penelitian ini dapat disimpulkan bahwa ASA dari serum wanita infertil dapat
menurunkan integritas membran plasma spermatozoa tergantung konsentrasi dan
waktu inkubasi secara signifikan. ASA juga dapat menurunkan persentase
fosforilasi tirosin, viabilitas dan motilitas spermatozoa tergantung konsentrasi dan
waktu inkubasi, namun penurunan ini secara statistik tidak signifikan.
<hr>
<B>ABTRACT</b><br>
One of the factors that determine the success of the fertilization process is the
ability of sperm to fertilize oocytes. Ejaculated sperm from the male reproductive
tract doesn?t have this capability. In the female reproductive tract (cervix) sperm
undergo a series of processes that make it has the ability to fertilize an oocyte,
called capacitation. Capacitation is an important step for successful fertilization
requires the integrity of the plasma membrane and characterized by tyrosine
phosphorylation. Definitely, sperm are foreign materials for the female and male
body that can stimulate an immune response in the form of antibodies to sperm
formation (ASA). This study aims to determine the effect of antisperm antibody
from infertile women sera on viability, motility, plasma membrane integrity, as
well as the intensity and localization of tyrosine phosphorylation in normal
spermatozoa. The design of this study is experimental in vitro. The washed
spermatozoa of normal fertile donors incubated with ASA from infertile women
sera, with different concentrations (0, 1/1000, 1/100 and 1/10) for 1 hour and 2
hours and then examined the parameters; plasma membrane integrity, intensity
and location of tyrosine phosphorylation, viability and motility of spermatozoa.
Test results for all measured parameters showed a similar trend, which is
incubation of spermatozoa with positive serum ASA with increasing
concentration causes a decrease in the percentage of plasma membrane integrity,
tyrosine phosphorylation, viability and motility of spermatozoa for 1 hour
incubation time, but not so for incubation for 2 hours . But overall all parameters
showed a lower percentage value for incubation for 2 hours than 1 hour. Thus, in
this study it can be concluded that the ASA from infertile women sera can lower
plasma membrane integrity of spermatozoa depending on concentration and
incubation time significantly. ASA can also reduce the percentage of tyrosine
phosphorylation, viability and motility of spermatozoa depending on
concentration and time of incubation, but this decrease was not statistically
significant., One of the factors that determine the success of the fertilization process is the
ability of sperm to fertilize oocytes. Ejaculated sperm from the male reproductive
tract doesn’t have this capability. In the female reproductive tract (cervix) sperm
undergo a series of processes that make it has the ability to fertilize an oocyte,
called capacitation. Capacitation is an important step for successful fertilization
requires the integrity of the plasma membrane and characterized by tyrosine
phosphorylation. Definitely, sperm are foreign materials for the female and male
body that can stimulate an immune response in the form of antibodies to sperm
formation (ASA). This study aims to determine the effect of antisperm antibody
from infertile women sera on viability, motility, plasma membrane integrity, as
well as the intensity and localization of tyrosine phosphorylation in normal
spermatozoa. The design of this study is experimental in vitro. The washed
spermatozoa of normal fertile donors incubated with ASA from infertile women
sera, with different concentrations (0, 1/1000, 1/100 and 1/10) for 1 hour and 2
hours and then examined the parameters; plasma membrane integrity, intensity
and location of tyrosine phosphorylation, viability and motility of spermatozoa.
Test results for all measured parameters showed a similar trend, which is
incubation of spermatozoa with positive serum ASA with increasing
concentration causes a decrease in the percentage of plasma membrane integrity,
tyrosine phosphorylation, viability and motility of spermatozoa for 1 hour
incubation time, but not so for incubation for 2 hours . But overall all parameters
showed a lower percentage value for incubation for 2 hours than 1 hour. Thus, in
this study it can be concluded that the ASA from infertile women sera can lower
plasma membrane integrity of spermatozoa depending on concentration and
incubation time significantly. ASA can also reduce the percentage of tyrosine
phosphorylation, viability and motility of spermatozoa depending on
concentration and time of incubation, but this decrease was not statistically
significant.]</description><subject>Spermatozoa</subject><subject>Cell membranes</subject><identifier>20404106</identifier><source>http://lib.ui.ac.id/file?file=digital/2015-12/20404106-T-Hajizah.pdf</source><recordID>20404106</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Bachelors |
| author |
Hajizah, author |
| title |
Pengaruh antibodi antisperma dari serum wanita infertil terhadap integritas membran plasma, fosforilasi tirosin, viabilitas dan motilitas spermatozoa manusia = Effects of antisperm antibody from infertile woman sera on plasma membrane integrity tyrosine phosphorylation viability and motility of human sperm |
| publisher |
[, Fakultas Kedokteran Universitas Indonesia] |
| publishDate |
2014 |
| topic |
Spermatozoa Cell membranes |
| url |
http://lib.ui.ac.id/file?file=digital/2015-12/20404106-T-Hajizah.pdf |
| contents |
[<b>ABSTRAK</b><br>
Salah satu faktor yang menentukan keberhasilan proses fertilisasi adalah
kemampuan sperma membuahi oosit. Sperma yang diejakulasikan dari saluran
reproduksi pria belum memiliki kemampuan ini. Di dalam saluran reproduksi
wanita (servik) sperma mengalami serangkaian proses yang membuatnya
memiliki kemampuan membuahi oosit yang disebut kapasitasi. Kapasitasi
merupakan tahapan penting bagi keberhasilan fertilisasi yang memerlukan
integritas membran plasma baik dan dikarakterisasi oleh fosforilasi tirosin.
Sementara itu, bagi tubuh perempuan maupun laki-laki spermatozoa merupakan
benda asing yang dapat merangsang respon imun berupa pembentukan antibodi
terhadap sperma (ASA). Penelitian ini bertujuan untuk mengetahui pengaruh
antibodi antisperma dari serum wanita infertil terhadap integritas membran
plasma, status dan lokalisasi fosforilasi tirosin, serta viabilitas dan motilitas
spermatozoa manusia. Desain penelitian ini adalah eksperimental in vitro di mana
spermatozoa normal donor fertil yang telah dicuci diberi perlakuan diinkubasi
dengan serum yang terdeteksi ASA dari wanita infertil dengan kelompok
konsentrasi serum 0, 1/1000, 1/100 dan 1/10 selama 1 jam dan 2 jam, kemudian
diperiksa parameter-parameter; integritas membran plasma, intensitas dan lokasi
fosforilasi tirosin serta viabilitas dan motilitas spermatozoa. Hasil pemeriksaan
untuk semua parameter yang diukur menunjukkan kecenderungan yang sama,
yaitu inkubasi spermatozoa dengan serum positif ASA dengan konsentrasi
meningkat menyebabkan penurunan persentase integritas membran plasma,
fosforilasi tirosin, viabilitas dan motilitas spermatozoa untuk waktu inkubasi 1
jam, namun tidak demikian untuk inkubasi selama 2 jam. Namun secara
keseluruhan semua parameter menunjukkan nilai persentase yang lebih rendah
untuk inkubasi selama 2 jam dibandingkan 1 jam. Dengan demikian pada
penelitian ini dapat disimpulkan bahwa ASA dari serum wanita infertil dapat
menurunkan integritas membran plasma spermatozoa tergantung konsentrasi dan
waktu inkubasi secara signifikan. ASA juga dapat menurunkan persentase
fosforilasi tirosin, viabilitas dan motilitas spermatozoa tergantung konsentrasi dan
waktu inkubasi, namun penurunan ini secara statistik tidak signifikan.
<hr>
<B>ABTRACT</b><br>
One of the factors that determine the success of the fertilization process is the
ability of sperm to fertilize oocytes. Ejaculated sperm from the male reproductive
tract doesn?t have this capability. In the female reproductive tract (cervix) sperm
undergo a series of processes that make it has the ability to fertilize an oocyte,
called capacitation. Capacitation is an important step for successful fertilization
requires the integrity of the plasma membrane and characterized by tyrosine
phosphorylation. Definitely, sperm are foreign materials for the female and male
body that can stimulate an immune response in the form of antibodies to sperm
formation (ASA). This study aims to determine the effect of antisperm antibody
from infertile women sera on viability, motility, plasma membrane integrity, as
well as the intensity and localization of tyrosine phosphorylation in normal
spermatozoa. The design of this study is experimental in vitro. The washed
spermatozoa of normal fertile donors incubated with ASA from infertile women
sera, with different concentrations (0, 1/1000, 1/100 and 1/10) for 1 hour and 2
hours and then examined the parameters; plasma membrane integrity, intensity
and location of tyrosine phosphorylation, viability and motility of spermatozoa.
Test results for all measured parameters showed a similar trend, which is
incubation of spermatozoa with positive serum ASA with increasing
concentration causes a decrease in the percentage of plasma membrane integrity,
tyrosine phosphorylation, viability and motility of spermatozoa for 1 hour
incubation time, but not so for incubation for 2 hours . But overall all parameters
showed a lower percentage value for incubation for 2 hours than 1 hour. Thus, in
this study it can be concluded that the ASA from infertile women sera can lower
plasma membrane integrity of spermatozoa depending on concentration and
incubation time significantly. ASA can also reduce the percentage of tyrosine
phosphorylation, viability and motility of spermatozoa depending on
concentration and time of incubation, but this decrease was not statistically
significant., One of the factors that determine the success of the fertilization process is the
ability of sperm to fertilize oocytes. Ejaculated sperm from the male reproductive
tract doesn’t have this capability. In the female reproductive tract (cervix) sperm
undergo a series of processes that make it has the ability to fertilize an oocyte,
called capacitation. Capacitation is an important step for successful fertilization
requires the integrity of the plasma membrane and characterized by tyrosine
phosphorylation. Definitely, sperm are foreign materials for the female and male
body that can stimulate an immune response in the form of antibodies to sperm
formation (ASA). This study aims to determine the effect of antisperm antibody
from infertile women sera on viability, motility, plasma membrane integrity, as
well as the intensity and localization of tyrosine phosphorylation in normal
spermatozoa. The design of this study is experimental in vitro. The washed
spermatozoa of normal fertile donors incubated with ASA from infertile women
sera, with different concentrations (0, 1/1000, 1/100 and 1/10) for 1 hour and 2
hours and then examined the parameters; plasma membrane integrity, intensity
and location of tyrosine phosphorylation, viability and motility of spermatozoa.
Test results for all measured parameters showed a similar trend, which is
incubation of spermatozoa with positive serum ASA with increasing
concentration causes a decrease in the percentage of plasma membrane integrity,
tyrosine phosphorylation, viability and motility of spermatozoa for 1 hour
incubation time, but not so for incubation for 2 hours . But overall all parameters
showed a lower percentage value for incubation for 2 hours than 1 hour. Thus, in
this study it can be concluded that the ASA from infertile women sera can lower
plasma membrane integrity of spermatozoa depending on concentration and
incubation time significantly. ASA can also reduce the percentage of tyrosine
phosphorylation, viability and motility of spermatozoa depending on
concentration and time of incubation, but this decrease was not statistically
significant.] |
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