Uji penghambatan aktivitas tirosinase dari minyak biji markisa kuning passiflora edulis f flavicarpa dan penentuan parameter minyak = Inhibition of tyrosinase activity test from yellow passion fruit seed oil passiflora edulis f flavicarpa and oil parameter determination / Dian Coryokto Damanik
| Main Author: | Damanik, Dian Coryokto, author |
|---|---|
| Format: | Bachelors |
| Terbitan: |
, 2014
|
| Subjects: | |
| Online Access: |
http://lib.ui.ac.id/file?file=digital/20368550-S53796-Dian Coryokto Damanik.pdf |
| ctrlnum |
20368550 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Uji penghambatan aktivitas tirosinase dari minyak biji markisa kuning passiflora edulis f flavicarpa dan penentuan parameter minyak = Inhibition of tyrosinase activity test from yellow passion fruit seed oil passiflora edulis f flavicarpa and oil parameter determination / Dian Coryokto Damanik</title><creator>Damanik, Dian Coryokto, author</creator><type>Thesis:Bachelors</type><place/><publisher/><date>2014</date><description><b>ABSTRAK</b><br>
Tirosinase merupakan suatu enzim yang mengkatalisis proses melanogenesis
dalam pembentukan pigmen melanin. Produksi melanin yang berlebihan
(hiperpigmentasi) dapat menyebabkan terjadinya penggelapan warna kulit.
Pengontrolan produksi melanin dapat dilakukan dengan cara menghambat
aktivitas enzim tirosinase. Minyak biji markisa kuning diduga memiliki efek
terhadap pencerahan kulit. Tujuan dari penelitian ini adalah untuk mengetahui
potensi penghambatan aktivitas tirosinase serta menentukan parameter kualitas
minyak biji markisa kuning. Pengujian penghambatan tirosinase oleh minyak biji
markisa kuning dilakukan dengan mengukur serapan L-dopakrom pada panjang
gelombang 490 nm. Minyak biji markisa kuning memiliki nilai IC50 195,342
&#956;g/mL. Pengujian kinetika enzim melalui plot Lineweaver-Burk menunjukkan
bahwa minyak biji markisa memiliki penghambatan kompetitif. Hasil penentuan
parameter minyak biji markisa menunjukkan hasil bobot jenis 0,8912 g/cm3,
indeks bias 1,472, bilangan asam 53,962 mgNaOH/g, bilangan penyabunan
186,64 mgKOH/g, bilangan iodium 375,5 g I2/ 100 g minyak, bilangan hidroksil
239,38 mg/g dan zat tidak tersabunkan 1,169, dan mengandung saponin, tanin,
serta glikosida
<hr>
<b>ABSTRACT</b><b>
Tyrosinase is an enzyme that catalyzes the process of melanogenesis which in
case of excessive melanin production (hyperpigmentation) may causes darkening
of skin color. Melanin production can be controlled by the inhibition of tyrosinase
enzyme. Yellow passion fruit seeds was investigated had an effect on the skin
whitening. This research used to determined the potential inhibition of tyrosinase
activity from yellow passion fruit seed oil and determined parameters of the
yellow passion fruit seed oil. The inhibition of tyrosinase activity test from yellow
passion fruit seed oil was done by measuring the wavelength of dopachrome at
490 nm. The test showed that yellow passion fruit seed oil had IC50 values of
195.342 &#956;g/mL. The inhibition kinetics analyzed by a Lineweaver-Burk plot
indicated that yellow passion fruit seed oil was a noncompetitive inhibitor.
Passion fruit seed oil had density of 0.8912 of g/cm3, refractive index of 1.472,
acid value of 53.962 mgNaOH/g, saponification value 186.64 of mg/g, iodine
value of 375.5 g I2/ 100 g oil, hydroxyl value of 239.38 mg/g, unsaponifiable
matter of 1.169, and contained saponins, tannins, and glycosides</description><subject>Melanins</subject><subject>Melanocytes.</subject><identifier>20368550</identifier><source>http://lib.ui.ac.id/file?file=digital/20368550-S53796-Dian Coryokto Damanik.pdf</source><recordID>20368550</recordID></dc>
|
| format |
Thesis:Bachelors Thesis |
| author |
Damanik, Dian Coryokto, author |
| title |
Uji penghambatan aktivitas tirosinase dari minyak biji markisa kuning passiflora edulis f flavicarpa dan penentuan parameter minyak = Inhibition of tyrosinase activity test from yellow passion fruit seed oil passiflora edulis f flavicarpa and oil parameter determination / Dian Coryokto Damanik |
| publishDate |
2014 |
| topic |
Melanins Melanocytes |
| url |
http://lib.ui.ac.id/file?file=digital/20368550-S53796-Dian Coryokto Damanik.pdf |
| contents |
<b>ABSTRAK</b><br>
Tirosinase merupakan suatu enzim yang mengkatalisis proses melanogenesis
dalam pembentukan pigmen melanin. Produksi melanin yang berlebihan
(hiperpigmentasi) dapat menyebabkan terjadinya penggelapan warna kulit.
Pengontrolan produksi melanin dapat dilakukan dengan cara menghambat
aktivitas enzim tirosinase. Minyak biji markisa kuning diduga memiliki efek
terhadap pencerahan kulit. Tujuan dari penelitian ini adalah untuk mengetahui
potensi penghambatan aktivitas tirosinase serta menentukan parameter kualitas
minyak biji markisa kuning. Pengujian penghambatan tirosinase oleh minyak biji
markisa kuning dilakukan dengan mengukur serapan L-dopakrom pada panjang
gelombang 490 nm. Minyak biji markisa kuning memiliki nilai IC50 195,342
μg/mL. Pengujian kinetika enzim melalui plot Lineweaver-Burk menunjukkan
bahwa minyak biji markisa memiliki penghambatan kompetitif. Hasil penentuan
parameter minyak biji markisa menunjukkan hasil bobot jenis 0,8912 g/cm3,
indeks bias 1,472, bilangan asam 53,962 mgNaOH/g, bilangan penyabunan
186,64 mgKOH/g, bilangan iodium 375,5 g I2/ 100 g minyak, bilangan hidroksil
239,38 mg/g dan zat tidak tersabunkan 1,169, dan mengandung saponin, tanin,
serta glikosida
<hr>
<b>ABSTRACT</b><b>
Tyrosinase is an enzyme that catalyzes the process of melanogenesis which in
case of excessive melanin production (hyperpigmentation) may causes darkening
of skin color. Melanin production can be controlled by the inhibition of tyrosinase
enzyme. Yellow passion fruit seeds was investigated had an effect on the skin
whitening. This research used to determined the potential inhibition of tyrosinase
activity from yellow passion fruit seed oil and determined parameters of the
yellow passion fruit seed oil. The inhibition of tyrosinase activity test from yellow
passion fruit seed oil was done by measuring the wavelength of dopachrome at
490 nm. The test showed that yellow passion fruit seed oil had IC50 values of
195.342 μg/mL. The inhibition kinetics analyzed by a Lineweaver-Burk plot
indicated that yellow passion fruit seed oil was a noncompetitive inhibitor.
Passion fruit seed oil had density of 0.8912 of g/cm3, refractive index of 1.472,
acid value of 53.962 mgNaOH/g, saponification value 186.64 of mg/g, iodine
value of 375.5 g I2/ 100 g oil, hydroxyl value of 239.38 mg/g, unsaponifiable
matter of 1.169, and contained saponins, tannins, and glycosides |
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Universitas Indonesia |
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JAWA BARAT |
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