Pengaruh pemberian minyak buah merah (Pandanus Conoideus Lam) pada hati tikus yang cedera akibat D-Galaktosamin
| Main Authors: | Tena Djuartina, author, Add author: Santoso Gunardi, supervisor, Add author: Frans D. Suyatna, supervisor |
|---|---|
| Format: | Masters Thesis |
| Terbitan: |
, 2006
|
| Subjects: | |
| Online Access: |
http://lontar.ui.ac.id/detail?id=115997 |
| ctrlnum |
115997 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Pengaruh pemberian minyak buah merah (Pandanus Conoideus Lam) pada hati tikus yang cedera akibat D-Galaktosamin</title><creator>Tena Djuartina, author</creator><creator>Add author: Santoso Gunardi, supervisor</creator><creator>Add author: Frans D. Suyatna, supervisor</creator><publisher/><date>2006</date><subject>Biomedical & nanomedical technologies</subject><description><b>ABSTRAK</b><br>
Objektif
Tujuan penelitian ini melihat pengaruh minyak buah merah Pandanus conoideus Lam
pada hati tikus yang cedera akibat D galaktosamin
Metode
Penelitian ini dilakukan terhadap tikus putih jantan strain Sprague Dawley berumur 2 3
bulan dengan berat badan 120 150 gram Pada penelitian ini digunakan rancangan
acak Dibagi dalam lima kelompok masing masing kelompok enam ekor mendapat
perlakuan selama empat minggu Kelompok kontrol (kelompok 1) diberi air kelompok
diberi MBM (kelompok 2) kelompok diberi D galaktosamin (Kelompok 3) kelompok
diberi minyak buah merah selama satu minggu kemudian diberikan D galaktosamin
(Kelompok 4) kelompok diben minyak buah merah dan D galaktosamin secara
bersamaan (Kelompok 5) Dosis MBM yang digunakan 1 ml/ kgBB/han per oral dosis D
galaktosamin 200 mg/KgBB/minggu secara intrapentoneal Parameter yang diuji adalah
MDA plasma MDA hati GPT plasma GPT hati berat badan berat hati dan gambaran
histopatologik hati Data hasil pengukuran antara kelompok perlakuan dilakukan dengan
mengukur koefisien vanan Hasil data berdistnbusi normal dilanjutkan uji parametrik 1
way Anova kemudian dengan uji post hoc Turkey Hasil data perbandingan tiap minggu
yang berdistnbusi normal dilakukan uji parametrik 2 way Anova kemudian dilanjutkan
dengan uji multiple komparasi Bonferrom Hasil data berdistnbusi tidak normal maka
dianalisa dengan uji non parametnk Kruskall wallis dan dilanjutkan dengan uji Tamhane
Data yang diperoleh dan pembacaan skala diolah dengan cara krostabulasi kemudian
dilanjutkan dengan uji Chi Sguare
Hasil
Hasil pengukuran MDA plasma menunjukan D galaktosamin ini dapat
meningkatkan MDA plasma setiap minggunya hasil ini menunjukan bahwa D
galaktosamin mengakibatkan kerusakan oksidatif molekul lipid sejak awal pengamatan
pada minggu pertama Tampaknya efek protektif MBM terhadap D galaktosamin masih
ada pada minggu pertama hal ini mungkin disebabkan oleh antioksidan yang terdapat
dalam MBM pada minggu pertama masih dapat menetralisir stress oksidatif yang
ditimbulkan oleh D galaktosamin Disamping itu mungkin D galaktosamin belum bekerja
maksimal merusak pada minggu pertama Pada kelompok MBM + D galaktosamin
hasil MDA plasma lebih tinggi dibandingkan kelompok D galaktosamin mungkin ini
dikarenakan stress oksidatif yang ditimbulkan MBM + D galaktosamin lebih tinggi dibandingkan D galaktosamin itu sendiri Secara statistik MDA jaringan hati
menunjukkan D galaktosamin mengakibatkan kerusakan oksidatif Juga pada MBM
sendiri menyebabkan stress oksidatif sehingga bila diberikan bersamaan dengan D
galaktosamin kerusakan yang diakibatkannya menjadi lebih tinggi dibandingkan dengan
hanya diberi D galaktosamin Hasil MDA jaringan hati menunjukkan bahwa MBM
bersifat toksik terhadap hati sehingga menyebabkan peroksidasi lipid
Dari hasil pemenksaan GPT plasma disimpulkan D galaktosamin mempunyai
efek merusak hati hasil yang didapat juga mulai terlihat pada minggu ke 2 dan bila
diberi bersamaan dengan MBM ternyata GPT plasma melonjak lebih tinggi
Peningkatan ini mengindikasikan bahwa MBM berpotensi merusak sel hati Hasil
pemeriksaan GPT janngan hati juga menunjukkan D galaktosamin menyebabkan
kerusakan janngan hati dan MBM sendm membuat kerusakan struktur sehingga bila
diberi lebih lama yaitu satu minggu sebelumnya yang dimaksudkan untuk perlindungan
ternyata kerusakan yang terjadi lebih tinggi
Dari hasil pengukuran berat hati disimpulkan bahwa D galaktosamin ini
meningkatkan berat hati secara bermakna karena D galaktosamin ini mempunyai efek
merusak sel hati Dan MBM juga menunjukkan terjadinya peningkatan berat hati jadi
disimpulkan MBM tidak dapat memben perlindungan terhadap sel hati
Hasil pengukuran berat badan menunjukkan D galaktosamin menyebabkan
penurunan berat badan tapi sangat mengherankan ternyata bila MBM diberikan satu
minggu sebelumnya menyebabkan peningkatan berat badan mungkin disim karena
MBM mengandung multivitamin yang menyebabkan keinginan untuk makan
meningkat Bila diberikan bersamaan MBM dan D-galaktosamin ternyata menunjukkan
bahwa dengan pembenan MBM tersebut berat badan tidak dapat berubah secara
bermakna kemungkinan ini karena efek dari MBM tidak dapat menetralisir efek dari D
galaktosamin Efek MBM sendiri secara statistik tidak dapat meningkatkan berat badan
Pada pemenksaan histopatologi hasil yang didapat tidak terlalu mencolok antar
kelompok Hal tersebut cukup mendukung hasil pemenksaan GPT plasma maupun
GPT hati walaupun terjadi perubahan secara biokimia dan fisiologi tapi mungkin
belum mengakibatkan kerusakan organik yang bermakna secara histopatologi
Kerusakan anatomi akan didapat bila zat yang dipakai berlebihan dalam jangka yang
lama
Kesimpulan
Minyak buah merah tidak mempunyai efek protektif terhadap D galaktosamin.
<hr>
<b>ABSTRACT</b><br>
Backgroud
Red fruit (Pandanus conoideus Lam) oil is an antioxidant supplement which has
been reported to contain complete nutrient compositions including p carotene and a
tocopherol
Objectives
The aim of this study was to investigate the effect of red fruit oil on rat livers
injured by D galactosamine
Methods
This study was conducted on 2 3 months old male rats of Sprague Dawley strain
each weighing about 120 150 grams We used randomized samples We divided the
rats into five groups each group consisted of six rats Each group received a different
treatment for four weeks Group 1 (control) only received water group 2 received red
fruit oil Group 3 received D galactosamine Group 4 received red fruit oil for one week
earlier and then continued with D galactosamine Group 5 received red fruit oil along
with D galactosamine The red fruit oil supplement was given orally 1 ml/ kg BW/day for
4 weeks D galactosamine was given intrapentonealy 200 mg/kg BW/ week Every week
blood samples were obtained to measure the plasma MDA and plasma GPT levels After
four weeks blood samples and liver tissues were obtained to measure the plasma MDA
liver MDA plasma GPT liver GPT body weight liver weight and histopathological
feature of liver were determined as parameters The obtained values were analyzed
using parametric test 1 way Anova and continued with post Turkey hoc test The data
results with normal distnbution were compared every week then parametnc tests 2 way
Anova was conduted and continued with test of Bonferrom multiple comparisons The
data which were analyzed by Kruskal Wallis and Tamhane test showed that the
distnbution was normal The values obtained by scale reading were analyzed using
crosstabulation method and continued with test of Chi-Square
Results
The measurement of plasma MDA every week after treatment with D
galactosamine showed an increase of plasma MDA This result showed that D
galactosamine causes oxidative damage to lipid molecule since in early perception at
first week The protective effect of MBM to D galactosamine was seen at the first week
This effect was presumably caused by the antioxidative effects of MBM which
neutralized the oxidative stress induced by D galactosamine Also it was possible that
the peak toxic effect of D galactosamine had not appeared during the first week of the
study The plasma MDA level of group 4 dan 5 were higher than that of group 3 possibly because oxidative stress generated by MBM + D galactosamine was higher than the D
galactosamme itself
The examination of the tissue liver MDA statistically showed that D
galactosamine caused oxidative damage MBM alone also caused oxidative stress so
when it was co admimstered with higher D galactosamine the result was higher
plasma level of MDA compared to D galactosamine alone The result from the tissue
liver MDA indicated that MBM did not provide protection effect to the liver because
MBM caused lipid peroxidation
Examination of plasma GPT suggested that D galactosamine had damaging
effect to the liver The same results could also be seen at the second week When D
galactosamine was given at the same time with MBM the result of plasma GPT was
even higher The increase of plasma level MDA showed that MBM had potential
damaging effect to liver cells Examination of GPT liver tissue also showed that D
galactosamine caused liver tissue damage and MBM alone could also damage the
structure of the liver Futhermore when MBM was given one week longer the damage
was even higher
D galactosamine increased liver weight sigmficantly It suggesed that D
galactosamine might cause damage of the liver Similarly to MBM alone increased liver
weight It could be concluded that MBM was not protective to the liver cell
D galactosamine caused weight loss However surprisingly enough when MBM
was given one week before it increased of body weight This was possible because
MBM contains multivitamine that increased the appetite But when MBM and D
galactosamine were given at the same time the body weight did not change
sigmficantly It could happen because MBM did not neutralize the effect of D
galactosamine Statistically MBM alone coud not increase the body weight
The result of histopathologic examination showed insigmficant difference
between groups This result supported the examination of plasma GPT and also liver
GPT Even though there were biochemical and physiological changes
histopathologically there was no organ damage Histopatological damage would be
found when the substance was used in the long term period
Conclusion
These results suggested that red fruit oil did not have protective effect D
galactosamine.</description><identifier>http://lontar.ui.ac.id/detail?id=115997</identifier><recordID>115997</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Thesis |
| author |
Tena Djuartina, author Add author: Santoso Gunardi, supervisor Add author: Frans D. Suyatna, supervisor |
| title |
Pengaruh pemberian minyak buah merah (Pandanus Conoideus Lam) pada hati tikus yang cedera akibat D-Galaktosamin |
| publishDate |
2006 |
| topic |
Biomedical & nanomedical technologies |
| url |
http://lontar.ui.ac.id/detail?id=115997 |
| contents |
<b>ABSTRAK</b><br>
Objektif
Tujuan penelitian ini melihat pengaruh minyak buah merah Pandanus conoideus Lam
pada hati tikus yang cedera akibat D galaktosamin
Metode
Penelitian ini dilakukan terhadap tikus putih jantan strain Sprague Dawley berumur 2 3
bulan dengan berat badan 120 150 gram Pada penelitian ini digunakan rancangan
acak Dibagi dalam lima kelompok masing masing kelompok enam ekor mendapat
perlakuan selama empat minggu Kelompok kontrol (kelompok 1) diberi air kelompok
diberi MBM (kelompok 2) kelompok diberi D galaktosamin (Kelompok 3) kelompok
diberi minyak buah merah selama satu minggu kemudian diberikan D galaktosamin
(Kelompok 4) kelompok diben minyak buah merah dan D galaktosamin secara
bersamaan (Kelompok 5) Dosis MBM yang digunakan 1 ml/ kgBB/han per oral dosis D
galaktosamin 200 mg/KgBB/minggu secara intrapentoneal Parameter yang diuji adalah
MDA plasma MDA hati GPT plasma GPT hati berat badan berat hati dan gambaran
histopatologik hati Data hasil pengukuran antara kelompok perlakuan dilakukan dengan
mengukur koefisien vanan Hasil data berdistnbusi normal dilanjutkan uji parametrik 1
way Anova kemudian dengan uji post hoc Turkey Hasil data perbandingan tiap minggu
yang berdistnbusi normal dilakukan uji parametrik 2 way Anova kemudian dilanjutkan
dengan uji multiple komparasi Bonferrom Hasil data berdistnbusi tidak normal maka
dianalisa dengan uji non parametnk Kruskall wallis dan dilanjutkan dengan uji Tamhane
Data yang diperoleh dan pembacaan skala diolah dengan cara krostabulasi kemudian
dilanjutkan dengan uji Chi Sguare
Hasil
Hasil pengukuran MDA plasma menunjukan D galaktosamin ini dapat
meningkatkan MDA plasma setiap minggunya hasil ini menunjukan bahwa D
galaktosamin mengakibatkan kerusakan oksidatif molekul lipid sejak awal pengamatan
pada minggu pertama Tampaknya efek protektif MBM terhadap D galaktosamin masih
ada pada minggu pertama hal ini mungkin disebabkan oleh antioksidan yang terdapat
dalam MBM pada minggu pertama masih dapat menetralisir stress oksidatif yang
ditimbulkan oleh D galaktosamin Disamping itu mungkin D galaktosamin belum bekerja
maksimal merusak pada minggu pertama Pada kelompok MBM + D galaktosamin
hasil MDA plasma lebih tinggi dibandingkan kelompok D galaktosamin mungkin ini
dikarenakan stress oksidatif yang ditimbulkan MBM + D galaktosamin lebih tinggi dibandingkan D galaktosamin itu sendiri Secara statistik MDA jaringan hati
menunjukkan D galaktosamin mengakibatkan kerusakan oksidatif Juga pada MBM
sendiri menyebabkan stress oksidatif sehingga bila diberikan bersamaan dengan D
galaktosamin kerusakan yang diakibatkannya menjadi lebih tinggi dibandingkan dengan
hanya diberi D galaktosamin Hasil MDA jaringan hati menunjukkan bahwa MBM
bersifat toksik terhadap hati sehingga menyebabkan peroksidasi lipid
Dari hasil pemenksaan GPT plasma disimpulkan D galaktosamin mempunyai
efek merusak hati hasil yang didapat juga mulai terlihat pada minggu ke 2 dan bila
diberi bersamaan dengan MBM ternyata GPT plasma melonjak lebih tinggi
Peningkatan ini mengindikasikan bahwa MBM berpotensi merusak sel hati Hasil
pemeriksaan GPT janngan hati juga menunjukkan D galaktosamin menyebabkan
kerusakan janngan hati dan MBM sendm membuat kerusakan struktur sehingga bila
diberi lebih lama yaitu satu minggu sebelumnya yang dimaksudkan untuk perlindungan
ternyata kerusakan yang terjadi lebih tinggi
Dari hasil pengukuran berat hati disimpulkan bahwa D galaktosamin ini
meningkatkan berat hati secara bermakna karena D galaktosamin ini mempunyai efek
merusak sel hati Dan MBM juga menunjukkan terjadinya peningkatan berat hati jadi
disimpulkan MBM tidak dapat memben perlindungan terhadap sel hati
Hasil pengukuran berat badan menunjukkan D galaktosamin menyebabkan
penurunan berat badan tapi sangat mengherankan ternyata bila MBM diberikan satu
minggu sebelumnya menyebabkan peningkatan berat badan mungkin disim karena
MBM mengandung multivitamin yang menyebabkan keinginan untuk makan
meningkat Bila diberikan bersamaan MBM dan D-galaktosamin ternyata menunjukkan
bahwa dengan pembenan MBM tersebut berat badan tidak dapat berubah secara
bermakna kemungkinan ini karena efek dari MBM tidak dapat menetralisir efek dari D
galaktosamin Efek MBM sendiri secara statistik tidak dapat meningkatkan berat badan
Pada pemenksaan histopatologi hasil yang didapat tidak terlalu mencolok antar
kelompok Hal tersebut cukup mendukung hasil pemenksaan GPT plasma maupun
GPT hati walaupun terjadi perubahan secara biokimia dan fisiologi tapi mungkin
belum mengakibatkan kerusakan organik yang bermakna secara histopatologi
Kerusakan anatomi akan didapat bila zat yang dipakai berlebihan dalam jangka yang
lama
Kesimpulan
Minyak buah merah tidak mempunyai efek protektif terhadap D galaktosamin.
<hr>
<b>ABSTRACT</b><br>
Backgroud
Red fruit (Pandanus conoideus Lam) oil is an antioxidant supplement which has
been reported to contain complete nutrient compositions including p carotene and a
tocopherol
Objectives
The aim of this study was to investigate the effect of red fruit oil on rat livers
injured by D galactosamine
Methods
This study was conducted on 2 3 months old male rats of Sprague Dawley strain
each weighing about 120 150 grams We used randomized samples We divided the
rats into five groups each group consisted of six rats Each group received a different
treatment for four weeks Group 1 (control) only received water group 2 received red
fruit oil Group 3 received D galactosamine Group 4 received red fruit oil for one week
earlier and then continued with D galactosamine Group 5 received red fruit oil along
with D galactosamine The red fruit oil supplement was given orally 1 ml/ kg BW/day for
4 weeks D galactosamine was given intrapentonealy 200 mg/kg BW/ week Every week
blood samples were obtained to measure the plasma MDA and plasma GPT levels After
four weeks blood samples and liver tissues were obtained to measure the plasma MDA
liver MDA plasma GPT liver GPT body weight liver weight and histopathological
feature of liver were determined as parameters The obtained values were analyzed
using parametric test 1 way Anova and continued with post Turkey hoc test The data
results with normal distnbution were compared every week then parametnc tests 2 way
Anova was conduted and continued with test of Bonferrom multiple comparisons The
data which were analyzed by Kruskal Wallis and Tamhane test showed that the
distnbution was normal The values obtained by scale reading were analyzed using
crosstabulation method and continued with test of Chi-Square
Results
The measurement of plasma MDA every week after treatment with D
galactosamine showed an increase of plasma MDA This result showed that D
galactosamine causes oxidative damage to lipid molecule since in early perception at
first week The protective effect of MBM to D galactosamine was seen at the first week
This effect was presumably caused by the antioxidative effects of MBM which
neutralized the oxidative stress induced by D galactosamine Also it was possible that
the peak toxic effect of D galactosamine had not appeared during the first week of the
study The plasma MDA level of group 4 dan 5 were higher than that of group 3 possibly because oxidative stress generated by MBM + D galactosamine was higher than the D
galactosamme itself
The examination of the tissue liver MDA statistically showed that D
galactosamine caused oxidative damage MBM alone also caused oxidative stress so
when it was co admimstered with higher D galactosamine the result was higher
plasma level of MDA compared to D galactosamine alone The result from the tissue
liver MDA indicated that MBM did not provide protection effect to the liver because
MBM caused lipid peroxidation
Examination of plasma GPT suggested that D galactosamine had damaging
effect to the liver The same results could also be seen at the second week When D
galactosamine was given at the same time with MBM the result of plasma GPT was
even higher The increase of plasma level MDA showed that MBM had potential
damaging effect to liver cells Examination of GPT liver tissue also showed that D
galactosamine caused liver tissue damage and MBM alone could also damage the
structure of the liver Futhermore when MBM was given one week longer the damage
was even higher
D galactosamine increased liver weight sigmficantly It suggesed that D
galactosamine might cause damage of the liver Similarly to MBM alone increased liver
weight It could be concluded that MBM was not protective to the liver cell
D galactosamine caused weight loss However surprisingly enough when MBM
was given one week before it increased of body weight This was possible because
MBM contains multivitamine that increased the appetite But when MBM and D
galactosamine were given at the same time the body weight did not change
sigmficantly It could happen because MBM did not neutralize the effect of D
galactosamine Statistically MBM alone coud not increase the body weight
The result of histopathologic examination showed insigmficant difference
between groups This result supported the examination of plasma GPT and also liver
GPT Even though there were biochemical and physiological changes
histopathologically there was no organ damage Histopatological damage would be
found when the substance was used in the long term period
Conclusion
These results suggested that red fruit oil did not have protective effect D
galactosamine. |
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IOS18066.115997 |
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Universitas Indonesia |
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51 |
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Perpustakaan Universitas Indonesia |
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492 |
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contoh Repository Tesis (Open) Universitas Indonesia |
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18066 |
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KOTA DEPOK |
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JAWA BARAT |
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IOS18066 |
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2022-12-14T02:42:47Z |
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