Preparasi dan karakterisasi film bioadhesif untuk penghantaran insulin dengan menggunakan Kitosan-NAsetil sistein = Preparation and characterization of bioadhesive film for buccal delivery of insulin using chitosan-N-Acetyl cysteine
| Main Authors: | Pietradewi Hartrianti, author, Add author: Silvia Surini, supervisor, Add author: Effionora Anwar, supervisor, Add author: Iskandarsyah, examiner, Add author: Arry Yanuar, examiner, Add author: Joshita Djajadisastra, examiner |
|---|---|
| Format: | Masters Doctoral |
| Terbitan: |
Universitas Indonesia
, 2011
|
| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20293909 |
| ctrlnum |
20293909 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Preparasi dan karakterisasi film bioadhesif untuk penghantaran insulin dengan menggunakan Kitosan-NAsetil sistein = Preparation and characterization of bioadhesive film for buccal delivery of insulin using chitosan-N-Acetyl cysteine</title><creator>Pietradewi Hartrianti, author</creator><creator>Add author: Silvia Surini, supervisor</creator><creator>Add author: Effionora Anwar, supervisor</creator><creator>Add author: Iskandarsyah, examiner</creator><creator>Add author: Arry Yanuar, examiner</creator><creator>Add author: Joshita Djajadisastra, examiner</creator><publisher>Universitas Indonesia</publisher><date>2011</date><subject>Insulin</subject><description>[<b>ABSTRAK</b><>br>
Penggunaan film mukoadhesif dengan sifat fleksibel dan berpori yang diproduksi
dari kitosan-N-asetil sistein sebagai sistem penghantaran insulin melalui rute
bukal telah diteliti. Kitosan-N-asetil sistein (KNAC) didapatkan melalui reaksi
antara kitosan dengan N-asetil sistein yang dimediasi karbodiimida. Hasil sintesis
KNAC kemudian dikarakterisasi dalam hal jumlah gugus tiol, spektrum IR, serta
kemampuan mengembang. KNAC tersebut kemudian dibuat menjadi sediaan film
dengan metode solvent casting yang dilanjutkan dengan pengeringan beku. Film
yang dihasilkan kemudian dikarakterisasi morfologi permukaan film serta
kekuatan dan waktu mukoadhesinya. Selain itu, pelepasan dan difusi insulin dari
film kNAC juga dilaksanakan pada penelitian ini. Untuk membandingkan efek
hipoglikemik in vivo, sejumlah tikus sehat diberikan film insulin KNAC secara
bukal dengan injeksi insulin subkutan sebagai pembandingnya. Hasil dari sintesis
KNAC menunjukkan kandungan gugus tiol bebas yang tinggi (423,28 ± 12,99
µmol per gram polimer) dengan kemampuan mengembang hingga 1,5 kali berat
awalnya. Film dari KNAC yg disintesis menunjukkan gaya mukoadhesi dan
waktu mukoadhesi secara berturut-turut hingga mencapai 1,25 kali dan 2,4 kali
dibandingkan film kitosan yang tidak dimodifikasi (p<0.05, t-test). Hasil uji juga
menunjukkan bahwa film KNAC dapat memberikan pelepasan dan difusi obat
secara berturut-turut hingga 95 % dalam 30 menit dan 70 % dalam 3 jam. Film
insulin KNAC yang diberikan melalui rute bukal mampu memberikan efek
hipoglikemik hingga 65,46 % dari kadar gula darah awal. Selain itu, hasil
availabilitas farmakologi dari film insulin KNAC terhadap injeksi insulin
subkutan mencapai 18,91%. Sebagai kesimpulan, penelitian ini memberikan
gambaran bahwa film kitosan-NAC memiliki potensi untuk dikembangkan
sebagai pembawa dalam penghantaran bukal insulin dan makromolekul lainnya.
<hr>
<b>Abstract</b><br>
The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 µmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 µmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 µmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 µmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules., The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 µmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.]</description><identifier>https://lib.ui.ac.id/detail?id=20293909</identifier><recordID>20293909</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Doctoral |
| author |
Pietradewi Hartrianti, author Add author: Silvia Surini, supervisor Add author: Effionora Anwar, supervisor Add author: Iskandarsyah, examiner Add author: Arry Yanuar, examiner Add author: Joshita Djajadisastra, examiner |
| title |
Preparasi dan karakterisasi film bioadhesif untuk penghantaran insulin dengan menggunakan Kitosan-NAsetil sistein = Preparation and characterization of bioadhesive film for buccal delivery of insulin using chitosan-N-Acetyl cysteine |
| publisher |
Universitas Indonesia |
| publishDate |
2011 |
| topic |
Insulin |
| url |
https://lib.ui.ac.id/detail?id=20293909 |
| contents |
[<b>ABSTRAK</b><>br>
Penggunaan film mukoadhesif dengan sifat fleksibel dan berpori yang diproduksi
dari kitosan-N-asetil sistein sebagai sistem penghantaran insulin melalui rute
bukal telah diteliti. Kitosan-N-asetil sistein (KNAC) didapatkan melalui reaksi
antara kitosan dengan N-asetil sistein yang dimediasi karbodiimida. Hasil sintesis
KNAC kemudian dikarakterisasi dalam hal jumlah gugus tiol, spektrum IR, serta
kemampuan mengembang. KNAC tersebut kemudian dibuat menjadi sediaan film
dengan metode solvent casting yang dilanjutkan dengan pengeringan beku. Film
yang dihasilkan kemudian dikarakterisasi morfologi permukaan film serta
kekuatan dan waktu mukoadhesinya. Selain itu, pelepasan dan difusi insulin dari
film kNAC juga dilaksanakan pada penelitian ini. Untuk membandingkan efek
hipoglikemik in vivo, sejumlah tikus sehat diberikan film insulin KNAC secara
bukal dengan injeksi insulin subkutan sebagai pembandingnya. Hasil dari sintesis
KNAC menunjukkan kandungan gugus tiol bebas yang tinggi (423,28 ± 12,99
μmol per gram polimer) dengan kemampuan mengembang hingga 1,5 kali berat
awalnya. Film dari KNAC yg disintesis menunjukkan gaya mukoadhesi dan
waktu mukoadhesi secara berturut-turut hingga mencapai 1,25 kali dan 2,4 kali
dibandingkan film kitosan yang tidak dimodifikasi (p<0.05, t-test). Hasil uji juga
menunjukkan bahwa film KNAC dapat memberikan pelepasan dan difusi obat
secara berturut-turut hingga 95 % dalam 30 menit dan 70 % dalam 3 jam. Film
insulin KNAC yang diberikan melalui rute bukal mampu memberikan efek
hipoglikemik hingga 65,46 % dari kadar gula darah awal. Selain itu, hasil
availabilitas farmakologi dari film insulin KNAC terhadap injeksi insulin
subkutan mencapai 18,91%. Sebagai kesimpulan, penelitian ini memberikan
gambaran bahwa film kitosan-NAC memiliki potensi untuk dikembangkan
sebagai pembawa dalam penghantaran bukal insulin dan makromolekul lainnya.
<hr>
<b>Abstract</b><br>
The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 μmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 μmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 μmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.;The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 μmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules., The development of a new porous and flexible mucoadhesive films based on
chitosan-N-acetyl cysteine (Ch-NAC) for potential buccal delivery system of
insulin was carried out in this study. Ch-NAC was synthesized from
carbodiimide-mediated reaction between chitosan and N-acetyl cysteine. The
obtained Ch-NAC was then characterized by determining the thiol group content,
FT-IR spectra and swelling ability. The Ch-NAC was then prepared into insulincontaining
film by a simple solvent casting method followed with subsequent
freeze drying. The resulting film was then observed in terms of morphology and
mucoadhesion properties. Moreover, the diffusion and release study of insulin
from the Ch-NAC film was also studied. The hypoglycaemic effect of buccally
administered insulin containing Ch-NAC film compared to subcutaneous insulin
injection was then observed using healthy rats. The obtained Ch-NAC showed
high free thiol group content (423,28 ± 12,99 μmol per gram polymer) and
swelling ability up to 1.5 times its initial weight. The insulin-containing Ch-NAC
films showed up to 1.25 and 2.4 times mucoadhesion force and mucoadhesion
time compared to chitosan film as blanks, respectively (p<0.05, t-test). The Ch-
NAC films were able to show sufficient diffusion of insulin up to 70 % within 3
hours and provide 95 % release of insulin within 30 minutes. The insulin-containg
Ch-NAC films which were buccally administered reached hypoglycemic effect up
to 65,46 % of initial blood glucose level. The insulin pharmacological availability
of the buccally administered Ch-NAC films compared to subcutaneous injection
were 18.91 %. These results suggested that Ch-NAC films were a promising
carrier for buccal delivery of insulin and other macromolecules.] |
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Universitas Indonesia |
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KOTA DEPOK |
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JAWA BARAT |
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