Induksi Respons Pertahanan Tanaman Tembakau (Nicotiana tabacum) oleh Lipopolisakarida Bakteri Pseudomonas syringae pv. tabaci dan Pseudomonas syringae pv. glycinea
| Main Authors: | Pipit Marianingsih, author, Add author: Andi Salamah, supervisor, Add author: Wibowo Mangunwardoyo, examiner, Add author: Lestari Rahayu, examiner |
|---|---|
| Format: | Masters Doctoral |
| Terbitan: |
, 2012
|
| Online Access: |
https://lib.ui.ac.id/detail?id=20314435 |
| ctrlnum |
20314435 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Induksi Respons Pertahanan Tanaman Tembakau (Nicotiana tabacum) oleh Lipopolisakarida Bakteri Pseudomonas syringae pv. tabaci dan Pseudomonas syringae pv. glycinea</title><creator>Pipit Marianingsih, author</creator><creator>Add author: Andi Salamah, supervisor</creator><creator>Add author: Wibowo Mangunwardoyo, examiner</creator><creator>Add author: Lestari Rahayu, examiner</creator><publisher/><date>2012</date><subject/><description><b>ABSTRAK</b><br>
Telah dilakukan penelitian yang bertujuan untuk menginduksi respons
pertahanan tanaman tembakau oleh lipopolisaakrida (LPS). LPS diekstraksi dari
bakteri Pseudomonas syringae pv. tabaci (Pta) dan P. syringae pv. glycinea (Pgl).
Respons pertahanan tanaman yang diamati adalah deposisi callose dan ekspresi
gen terkait pertahanan (PAL, HIN 1 dan HSR 203J). Untuk pengamatan deposisi
callose, daun tembakau diinfiltrasi dengan LPS Pta dan Pgl (400 µg/ml dan 800
µg/ml) serta diinkubasi selama 24 dan 48 jam. Selanjutnya, klorofil daun
diluruhkan menggunakan larutan laktofenol dan diwarnai dengan aniline blue.
Deposisi callose diamati dibawah mikroskop fluoresensi. Hasil pengamatan
menunjukkan LPS bakteri Pgl menginduksi deposisi callose lebih banyak
dibandingkan LPS bakteri Pta. Pengamatan ekspresi gen-gen terkait pertahanan
dilakukan pada daun tembakau yang diinfiltrasi dengan 400 µg/ml LPS bakteri
Pta and Pgl, serta diinkubasi selama 6 jam. Hasil RT-PCR terhadap daun
tembakau menunjukkan LPS bakteri Pta dan Pgl mampu menginduksi ekspsresi
gen HIN 1, tetapi tidak mampu menginduksi ekspresi gen PAL dan HSR 203J.
Gen HIN 1 terekspresi lebih kuat pada daun tembakau yang diinduksi oleh LPS
bakteri Pgl daripada LPS Pta. Hasil penelitian mengindikasikan bahwa LPS
bakteri Pgl menginduksi respons pertahanan daun tembakau lebih baik daripada
LPS bakteri Pta.
<hr>
<b>Abstract</b><br>
The aim of this study is to know the induction of tobacco defense
responses by using lipopolysaccharides (LPS) which extracted from two
phytopathogen, Pseudomonas syringae pv. tabaci (Pta) and P. syringae pv.
glycinea (Pgl). The plant defense responses that observed are callose deposition
and expression of defense-related genes (PAL, HIN 1 and HSR 203J). To detect
callose deposition, tobacco leaves were infiltrated with 400 µg/ml and 800 µg/ml
LPS Pta and Pgl, then incubated for 24 or 48 hr. Tobacco leaves were cleared in
lactophenol solution, stained with aniline blue, then visualized by fluorescence
microscopy. The result showed that LPS from Pgl induced more callose
deposition than that from Pta in tobacco leaves. To investigate defense-related
genes expression, tobacco leaves were infiltrated with 400 µg/ml LPS extracted
from Pta and Pgl, then incubated for 6 hr. Analysis of defense-related genes
expression were conducted by RT-PCR and visualized by electrophoresis on a
1.8% agarose gel. The result showed LPS Pta and Pgl can induce expression of
HIN 1 gene in tobacco leaves, but can not induce the PAL and HSR 203J genes.
The HIN 1 gene was highly expressed in tobacco leaves induced by LPS Pgl. The
result indicates that tobacco could effectively recognize LPS of nonhost pathogen
Pgl but not in host pathogen Pta.</description><identifier>https://lib.ui.ac.id/detail?id=20314435</identifier><recordID>20314435</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Doctoral |
| author |
Pipit Marianingsih, author Add author: Andi Salamah, supervisor Add author: Wibowo Mangunwardoyo, examiner Add author: Lestari Rahayu, examiner |
| title |
Induksi Respons Pertahanan Tanaman Tembakau (Nicotiana tabacum) oleh Lipopolisakarida Bakteri Pseudomonas syringae pv. tabaci dan Pseudomonas syringae pv. glycinea |
| publishDate |
2012 |
| url |
https://lib.ui.ac.id/detail?id=20314435 |
| contents |
<b>ABSTRAK</b><br>
Telah dilakukan penelitian yang bertujuan untuk menginduksi respons
pertahanan tanaman tembakau oleh lipopolisaakrida (LPS). LPS diekstraksi dari
bakteri Pseudomonas syringae pv. tabaci (Pta) dan P. syringae pv. glycinea (Pgl).
Respons pertahanan tanaman yang diamati adalah deposisi callose dan ekspresi
gen terkait pertahanan (PAL, HIN 1 dan HSR 203J). Untuk pengamatan deposisi
callose, daun tembakau diinfiltrasi dengan LPS Pta dan Pgl (400 μg/ml dan 800
μg/ml) serta diinkubasi selama 24 dan 48 jam. Selanjutnya, klorofil daun
diluruhkan menggunakan larutan laktofenol dan diwarnai dengan aniline blue.
Deposisi callose diamati dibawah mikroskop fluoresensi. Hasil pengamatan
menunjukkan LPS bakteri Pgl menginduksi deposisi callose lebih banyak
dibandingkan LPS bakteri Pta. Pengamatan ekspresi gen-gen terkait pertahanan
dilakukan pada daun tembakau yang diinfiltrasi dengan 400 μg/ml LPS bakteri
Pta and Pgl, serta diinkubasi selama 6 jam. Hasil RT-PCR terhadap daun
tembakau menunjukkan LPS bakteri Pta dan Pgl mampu menginduksi ekspsresi
gen HIN 1, tetapi tidak mampu menginduksi ekspresi gen PAL dan HSR 203J.
Gen HIN 1 terekspresi lebih kuat pada daun tembakau yang diinduksi oleh LPS
bakteri Pgl daripada LPS Pta. Hasil penelitian mengindikasikan bahwa LPS
bakteri Pgl menginduksi respons pertahanan daun tembakau lebih baik daripada
LPS bakteri Pta.
<hr>
<b>Abstract</b><br>
The aim of this study is to know the induction of tobacco defense
responses by using lipopolysaccharides (LPS) which extracted from two
phytopathogen, Pseudomonas syringae pv. tabaci (Pta) and P. syringae pv.
glycinea (Pgl). The plant defense responses that observed are callose deposition
and expression of defense-related genes (PAL, HIN 1 and HSR 203J). To detect
callose deposition, tobacco leaves were infiltrated with 400 μg/ml and 800 μg/ml
LPS Pta and Pgl, then incubated for 24 or 48 hr. Tobacco leaves were cleared in
lactophenol solution, stained with aniline blue, then visualized by fluorescence
microscopy. The result showed that LPS from Pgl induced more callose
deposition than that from Pta in tobacco leaves. To investigate defense-related
genes expression, tobacco leaves were infiltrated with 400 μg/ml LPS extracted
from Pta and Pgl, then incubated for 6 hr. Analysis of defense-related genes
expression were conducted by RT-PCR and visualized by electrophoresis on a
1.8% agarose gel. The result showed LPS Pta and Pgl can induce expression of
HIN 1 gene in tobacco leaves, but can not induce the PAL and HSR 203J genes.
The HIN 1 gene was highly expressed in tobacco leaves induced by LPS Pgl. The
result indicates that tobacco could effectively recognize LPS of nonhost pathogen
Pgl but not in host pathogen Pta. |
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Universitas Indonesia |
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51 |
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Perpustakaan Universitas Indonesia |
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Repository Disertasi (Membership) Universitas Indonesia |
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KOTA DEPOK |
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JAWA BARAT |
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IOS18068 |
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