Efek pemberian sel mononuklear darah tali pusat manusia secara intraarterial dan intravena pada neurogenesis jaringan otak tikus pasca stroke iskemia = The effect of human umbilical cord blood mononuclear cell intravenously and intraarterially delivery in neurogenesis of rat brain tissue post ischemic stroke
| Main Authors: | Isabella Kurnia Liem, Sastia Winda Astuti, Yetty Ramli |
|---|---|
| Format: | Masters xv, 60 hal; ill; 21 x 30 cm |
| Bahasa: | ind |
| Terbitan: |
Program Magister Ilmu Biomedik
, 2018
|
| Subjects: | |
| Online Access: |
http://library.fk.ui.ac.id//index.php?p=show_detail&id=24244 http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_efek-pemberian-sel-mononuklear-darah-tali-pusat-manusia-secara-intraar-20231101094743.jpg&width=200 http://library.fk.ui.ac.id//index.php?p=show_detail&id=24440 |
| ctrlnum |
slims-24244 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Efek pemberian sel mononuklear darah tali pusat manusia secara intraarterial dan intravena pada neurogenesis jaringan otak tikus pasca stroke iskemia = The effect of human umbilical cord blood mononuclear cell intravenously and intraarterially delivery in neurogenesis of rat brain tissue post ischemic stroke.</title><creator>Isabella Kurnia Liem</creator><creator>Sastia Winda Astuti</creator><creator>Yetty Ramli</creator><subject>NeuN</subject><subject>Sel mononuklear</subject><subject>intraarterial</subject><subject>Neurogenenis</subject><subject>ischemic stroke</subject><subject>intravena</subject><subject>umbilical cord blood mononuclear cell</subject><subject>Pasca Stroke</subject><subject>Neurogenesis</subject><relation>http://library.fk.ui.ac.id//index.php?p=show_detail&id=24440</relation><publisher>Program Magister Ilmu Biomedik</publisher><date>2018</date><language>ind</language><type>Thesis:Masters</type><identifier>http://library.fk.ui.ac.id//index.php?p=show_detail&id=24244</identifier><identifier>T18300fk</identifier><description>Latar belakang: Menurut WHO stroke merupakan penyebab kematian nomor dua di dunia, yaitu sebanyak sekitar 15 juta kasus dengan jenis kasus terbanyak adalah stroke iskemia. Populasi sel mononuklear (MNC) darah tali pusat manusia mengandung sel progenitor dan prekursor sel endotel dalam jumlah banyak yang sering digunakan dalam penelitian stroke. Dalam penelitian sebelumnya terbukti bahwa pemberian MNC darah tali pusat manusia secara intraarterial dan intravena dapat meningkatkan vaskulogenesis. Tujuan: Membandingkan efek neurogenesis dari pemberian MNC darah tali pusat manusia secara intraarterial dan intravena kepada tikus model stroke. Metode: Penelitian eksperimental menggunakan tikus model stroke iskemia (obstruksi arteri cerebri media, n=24). Hewan coba dibagi menjadi empat kelompok yaitu normal (kontrol), stroke dengan pemberian plasebo, stroke dengan pemberian MNC secara intraarterial dan stroke dengan pemberian MNC secara intravena. Dua minggu pasca perlakuan dilakukan terminasi dan pewarnaan sediaan otak dengan Hematoxylin Eosin (HE) untuk mengetahui perubahan bentuk sel dan morfologi jaringan serta dengan pewarnaan imunohistokimia menggunakan antibodi antiNeun untuk mengetahui jumlah neuron. Hasil: Dari hasil pewarnaan HE diketahui adanya gliosis dan shrunken cell pada area korteks tikus stroke. Terdapat perbedaan hasil yang bermakna antara kelompok yang diberi plasebo dengan yang diberi MNC. Dari hasil pewarnaan imunohistokimia juga didapatkan peningkatan jumlah neuron yang signifikan antara kedua kelompok tersebut. Tidak ada perbedaan jumlah neuron yang signifikan antara pemberian MNC darah tali pusat secara intraarterial dengan intravena. Kesimpulan: Pemberian MNC darah tali pusat manusia secara intraarterial dan intravena memiliki efek neurogenesis yang sama pada jaringan otak tikus pasca stroke iskemia. Kata kunci: Stroke iskemia, Sel mononuklear darah tali pusat, Neurogenesis, NeuN.Background: According to WHO, stroke is the second leading cause of death in the world (about 15 million cases) with ischemic stroke as the most common type. Human umbilical-cord-blood-derived mononuclear cell (MNC) population contains large numbers of progenitor cells and endothelial cell precursors that are frequently used in stroke studies. In previous studies it has been shown that intraarterial and intravenous administration of human umbilical cord blood MNC can increase vasculogenesis. Objective: Comparing the neurogenesis effect of intra-arterial with intravenous delivery of MNC to stroke model rats. Methods: Experimental study using ischemic stroke model Wistar mice (cerebral artery media obstruction, n = 24). The animals were divided into four groups: normal (control), stroke with placebo delivery, stroke with MNC intra-arterial delivery and stroke with MNC intravenous delivery. After two weeks treatment mice were terminated and histologic preparations of brain are made and stained with Hematoxylin Eosin (HE) to determine cellular and tissue morphology changes and with immunohistochemical staining using anti-Neun to determine the number of neurons. Result: Microscopic observation of HE staining slides showed existence of gliosis and shrunken cells in cortical area of stroke rats. There were significant differences in outcomes between the placebo-treated and MNC-treated groups. From the immunohistochemical staining results also obtained increasing number of neurogenesis effect. Key Words: Ischemic stroke, umbilical cord blood mononuclear cell, Neurogenesis, NeuN</description><coverage>Jakarta</coverage><identifier>http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_efek-pemberian-sel-mononuklear-darah-tali-pusat-manusia-secara-intraar-20231101094743.jpg&width=200</identifier><type>Other:xv, 60 hal; ill; 21 x 30 cm</type><subject>NONE</subject><image>http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_efek-pemberian-sel-mononuklear-darah-tali-pusat-manusia-secara-intraar-20231101094743.jpg&width=200</image><recordID>slims-24244</recordID></dc>
|
| language |
ind |
| format |
Thesis:Masters Thesis Other:xv, 60 hal; ill; 21 x 30 cm Other |
| author |
Isabella Kurnia Liem Sastia Winda Astuti Yetty Ramli |
| title |
Efek pemberian sel mononuklear darah tali pusat manusia secara intraarterial dan intravena pada neurogenesis jaringan otak tikus pasca stroke iskemia = The effect of human umbilical cord blood mononuclear cell intravenously and intraarterially delivery in neurogenesis of rat brain tissue post ischemic stroke |
| publisher |
Program Magister Ilmu Biomedik |
| publishDate |
2018 |
| isbn |
2023110109474 |
| topic |
NeuN Sel mononuklear intraarterial Neurogenenis ischemic stroke intravena umbilical cord blood mononuclear cell Pasca Stroke Neurogenesis NONE |
| url |
http://library.fk.ui.ac.id//index.php?p=show_detail&id=24244 http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_efek-pemberian-sel-mononuklear-darah-tali-pusat-manusia-secara-intraar-20231101094743.jpg&width=200 http://library.fk.ui.ac.id//index.php?p=show_detail&id=24440 |
| contents |
Latar belakang: Menurut WHO stroke merupakan penyebab kematian nomor dua di dunia, yaitu sebanyak sekitar 15 juta kasus dengan jenis kasus terbanyak adalah stroke iskemia. Populasi sel mononuklear (MNC) darah tali pusat manusia mengandung sel progenitor dan prekursor sel endotel dalam jumlah banyak yang sering digunakan dalam penelitian stroke. Dalam penelitian sebelumnya terbukti bahwa pemberian MNC darah tali pusat manusia secara intraarterial dan intravena dapat meningkatkan vaskulogenesis. Tujuan: Membandingkan efek neurogenesis dari pemberian MNC darah tali pusat manusia secara intraarterial dan intravena kepada tikus model stroke. Metode: Penelitian eksperimental menggunakan tikus model stroke iskemia (obstruksi arteri cerebri media, n=24). Hewan coba dibagi menjadi empat kelompok yaitu normal (kontrol), stroke dengan pemberian plasebo, stroke dengan pemberian MNC secara intraarterial dan stroke dengan pemberian MNC secara intravena. Dua minggu pasca perlakuan dilakukan terminasi dan pewarnaan sediaan otak dengan Hematoxylin Eosin (HE) untuk mengetahui perubahan bentuk sel dan morfologi jaringan serta dengan pewarnaan imunohistokimia menggunakan antibodi antiNeun untuk mengetahui jumlah neuron. Hasil: Dari hasil pewarnaan HE diketahui adanya gliosis dan shrunken cell pada area korteks tikus stroke. Terdapat perbedaan hasil yang bermakna antara kelompok yang diberi plasebo dengan yang diberi MNC. Dari hasil pewarnaan imunohistokimia juga didapatkan peningkatan jumlah neuron yang signifikan antara kedua kelompok tersebut. Tidak ada perbedaan jumlah neuron yang signifikan antara pemberian MNC darah tali pusat secara intraarterial dengan intravena. Kesimpulan: Pemberian MNC darah tali pusat manusia secara intraarterial dan intravena memiliki efek neurogenesis yang sama pada jaringan otak tikus pasca stroke iskemia. Kata kunci: Stroke iskemia, Sel mononuklear darah tali pusat, Neurogenesis, NeuN.Background: According to WHO, stroke is the second leading cause of death in the world (about 15 million cases) with ischemic stroke as the most common type. Human umbilical-cord-blood-derived mononuclear cell (MNC) population contains large numbers of progenitor cells and endothelial cell precursors that are frequently used in stroke studies. In previous studies it has been shown that intraarterial and intravenous administration of human umbilical cord blood MNC can increase vasculogenesis. Objective: Comparing the neurogenesis effect of intra-arterial with intravenous delivery of MNC to stroke model rats. Methods: Experimental study using ischemic stroke model Wistar mice (cerebral artery media obstruction, n = 24). The animals were divided into four groups: normal (control), stroke with placebo delivery, stroke with MNC intra-arterial delivery and stroke with MNC intravenous delivery. After two weeks treatment mice were terminated and histologic preparations of brain are made and stained with Hematoxylin Eosin (HE) to determine cellular and tissue morphology changes and with immunohistochemical staining using anti-Neun to determine the number of neurons. Result: Microscopic observation of HE staining slides showed existence of gliosis and shrunken cells in cortical area of stroke rats. There were significant differences in outcomes between the placebo-treated and MNC-treated groups. From the immunohistochemical staining results also obtained increasing number of neurogenesis effect. Key Words: Ischemic stroke, umbilical cord blood mononuclear cell, Neurogenesis, NeuN |
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Universitas Indonesia |
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51 |
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e-Library FKUI |
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MESH MEDICINE_HANDBOOKS FAKULTAS KEDOKTERAN National Library Of Medicine |
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JAKARTA PUSAT |
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DKI JAKARTA |
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2023-11-06T08:04:54Z |
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1781979163881635840 |
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17.608942 |