The Effects of Alpha-Mangostin Administration on Hepatic IRS-1 in Insulin Resistant Rat Model
| Main Author: | Iqbal Kevin Kyle |
|---|---|
| Format: | Book xii, 29 hlm. ; 21 x 30 cm |
| Bahasa: | eng |
| Terbitan: |
Program Pendidikan Dokter Umum S1 KKI
, 2021
|
| Subjects: | |
| Online Access: |
http://library.fk.ui.ac.id//index.php?p=show_detail&id=28642 http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_the-effects-of-alpha-mangostin-administration-on-hepatic-irs-1-in-insu-20230105135228.jpeg&width=200 |
| ctrlnum |
slims-28642 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>The Effects of Alpha-Mangostin Administration on Hepatic IRS-1 in Insulin Resistant Rat Model.</title><creator>Iqbal Kevin Kyle</creator><subject>hepatic tissue</subject><subject>IRS-1</subject><subject>alpha-mangostin</subject><subject>Insulin Resistance</subject><publisher>Program Pendidikan Dokter Umum S1 KKI</publisher><date>2021</date><language>eng</language><type>Book:Book</type><identifier>http://library.fk.ui.ac.id//index.php?p=show_detail&id=28642</identifier><identifier>S21231fk</identifier><description>Background: Insulin resistance (IR) is an abnormal cellular mechanism that is present in various metabolic disorder, particularly type-2 diabetes mellitus. This condition is closely related to downregulation of Insulin Receptor Substrate-1 (IRS-1). T2DM ranks seventh as the highest cause of disability and ninth in mortality worldwide. This research project was conducted to provide further understanding on the effects of alpha-mangostin on its therapeutic effect by increasing hepatic IRS-1 concentration. Method: This experiment was done by analyzing hepatic IRS-1 concentration of 36 Sprague-Dawley mice that were divided into 6 groups; group 1: control (given 8 weeks of standard diet), group 2: control + Alpha-mangostin 200 (200 mg/kg/day), group 3: IR (given high fat and high glucose diet for 3 weeks and injected by streptozotocin i.p at fourth week), group 4: IR + metformin 200, group 5: IR + Alpha-ΜG 100, group 6: IR + Alpha-ΜG 200. Through random sampling, 4 samples from each group were chosen and each sample’s hepatic IRS-1 were measured using ELISA method. Data acquired was analyzed using SPSS software version 26. Result: The analysis was done utilizing Welch’s ANOVA test with Games-Howell post hoc. No significant difference of IRS-1 concentration found between group 3 (IR) and group 5 (IR + Alpha-MANGOSTIN 100, p = 1 ( > 0.05)) and group 6 (IR + AlphaMANGOSTIN 200, p = 0.558)). Group 6 (IR + Alpha-MANGOSTIN 200, p = 0.558) shown to have a higher IRS-1 compared to group 5 (IR + Alpha-MANGOSTIN 100) although not significant. Conclusion: Alpha-mangsotin administration unable to increase IRS-1 concentration in insulin resistant mouse. Keywords: Insulin resistance, Alpha-mangostin, IRS-1, hepatic tissueLatar Belakang: Resistensi insulin adalah keadaan sel yang tidak normal. Keadaan ini ada pada banyak gangguan metabolik, termasuk diabetes tipe-2. Kondisi ini berkaitan erat dengan penurunan Insulin receptor substrate 1 (IRS-1). Penelitian ini bertujuan untuk mengevaluasi kemampuan dari Alfa-mangostin dalam meningkatkan konsenstrasi IRS-1 pada jaringan hati tikus model resistensi insulin. Metode: 36 tikus Sprague-Dawley dibagi ke dalam 6 kelompok; kelompok 1: kontrol (diberikan diet normal selama 8 minggu), kelompok 2; kontrol + alfa-mangostin 200 (200 mg/kg/hari), kelompok 3; resisten insulin (diberikan diet tinggi lemak dan gula selama 3 minggu dan diinjeksi dengan streptozotocin intraperitoneal dosis rendah pada minggu ke 3), kelompok 4: resisten insulin + metformin, kelompok 5: resisten insulin + alfamangostin 100, kelompok 6: resisten insulin + alfa-mangostin 200. Pada masing-masing kelompok dipilih 4 sampel secara acak yang kemudian dikorbankan setelah 8 minggu. Kemudian jaringan hati diambil, diisoloasi, dan diukur konsentrasi IRS-1 menggunakan metode ELISA. Data yang didapat kemudian dianalisa menggunakan SPSS versi 26. Hasil: Analisis dilakukan dengan uji Welch’s ANOVA dan Games-Howell post hoc. Tidak ditemukan adanya perbedaan signifikan antara perbedaan konsentrasi IRS-1 hati pada kelompok 3 (resisten insulin) dan kelompok 5 dan 6 (Alpha-ΜG 100, p = 1 ( > 0.05) dan Alpha-MANGOSTIN 200, p = 0.677 ( > 0.05)). Kelompok 6 memiliki konsenstrasi IRS-1 lebih tinggi dari kelompok 5, meskipun tidak secara signifikan (p = 0.558, ( > 0.05)). Kesimpulan: Pemberian alpha-mangostin 100 Mangostin dan 200 Mangostin tidak dapat meningkatkan konsentrasi IRS-1 pada hati. Kata kunci: Resistensi insulin, Alfa-mangostin, IRS-1, jaringan hati</description><coverage>Jakarta</coverage><identifier>http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_the-effects-of-alpha-mangostin-administration-on-hepatic-irs-1-in-insu-20230105135228.jpeg&width=200</identifier><type>Other:xii, 29 hlm. ; 21 x 30 cm</type><subject>NONE</subject><image>http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_the-effects-of-alpha-mangostin-administration-on-hepatic-irs-1-in-insu-20230105135228.jpeg&width=200</image><recordID>slims-28642</recordID></dc>
|
| language |
eng |
| format |
Book:Book Book Other:xii, 29 hlm. ; 21 x 30 cm Other |
| author |
Iqbal Kevin Kyle |
| title |
The Effects of Alpha-Mangostin Administration on Hepatic IRS-1 in Insulin Resistant Rat Model |
| publisher |
Program Pendidikan Dokter Umum S1 KKI |
| publishDate |
2021 |
| isbn |
2023010513522 |
| topic |
hepatic tissue IRS-1 alpha-mangostin Insulin Resistance NONE |
| url |
http://library.fk.ui.ac.id//index.php?p=show_detail&id=28642 http://library.fk.ui.ac.id//lib/minigalnano/createthumb.php?filename=images/docs/cover_the-effects-of-alpha-mangostin-administration-on-hepatic-irs-1-in-insu-20230105135228.jpeg&width=200 |
| contents |
Background: Insulin resistance (IR) is an abnormal cellular mechanism that is present in various metabolic disorder, particularly type-2 diabetes mellitus. This condition is closely related to downregulation of Insulin Receptor Substrate-1 (IRS-1). T2DM ranks seventh as the highest cause of disability and ninth in mortality worldwide. This research project was conducted to provide further understanding on the effects of alpha-mangostin on its therapeutic effect by increasing hepatic IRS-1 concentration. Method: This experiment was done by analyzing hepatic IRS-1 concentration of 36 Sprague-Dawley mice that were divided into 6 groups; group 1: control (given 8 weeks of standard diet), group 2: control + Alpha-mangostin 200 (200 mg/kg/day), group 3: IR (given high fat and high glucose diet for 3 weeks and injected by streptozotocin i.p at fourth week), group 4: IR + metformin 200, group 5: IR + Alpha-ΜG 100, group 6: IR + Alpha-ΜG 200. Through random sampling, 4 samples from each group were chosen and each sample’s hepatic IRS-1 were measured using ELISA method. Data acquired was analyzed using SPSS software version 26. Result: The analysis was done utilizing Welch’s ANOVA test with Games-Howell post hoc. No significant difference of IRS-1 concentration found between group 3 (IR) and group 5 (IR + Alpha-MANGOSTIN 100, p = 1 ( > 0.05)) and group 6 (IR + AlphaMANGOSTIN 200, p = 0.558)). Group 6 (IR + Alpha-MANGOSTIN 200, p = 0.558) shown to have a higher IRS-1 compared to group 5 (IR + Alpha-MANGOSTIN 100) although not significant. Conclusion: Alpha-mangsotin administration unable to increase IRS-1 concentration in insulin resistant mouse. Keywords: Insulin resistance, Alpha-mangostin, IRS-1, hepatic tissueLatar Belakang: Resistensi insulin adalah keadaan sel yang tidak normal. Keadaan ini ada pada banyak gangguan metabolik, termasuk diabetes tipe-2. Kondisi ini berkaitan erat dengan penurunan Insulin receptor substrate 1 (IRS-1). Penelitian ini bertujuan untuk mengevaluasi kemampuan dari Alfa-mangostin dalam meningkatkan konsenstrasi IRS-1 pada jaringan hati tikus model resistensi insulin. Metode: 36 tikus Sprague-Dawley dibagi ke dalam 6 kelompok; kelompok 1: kontrol (diberikan diet normal selama 8 minggu), kelompok 2; kontrol + alfa-mangostin 200 (200 mg/kg/hari), kelompok 3; resisten insulin (diberikan diet tinggi lemak dan gula selama 3 minggu dan diinjeksi dengan streptozotocin intraperitoneal dosis rendah pada minggu ke 3), kelompok 4: resisten insulin + metformin, kelompok 5: resisten insulin + alfamangostin 100, kelompok 6: resisten insulin + alfa-mangostin 200. Pada masing-masing kelompok dipilih 4 sampel secara acak yang kemudian dikorbankan setelah 8 minggu. Kemudian jaringan hati diambil, diisoloasi, dan diukur konsentrasi IRS-1 menggunakan metode ELISA. Data yang didapat kemudian dianalisa menggunakan SPSS versi 26. Hasil: Analisis dilakukan dengan uji Welch’s ANOVA dan Games-Howell post hoc. Tidak ditemukan adanya perbedaan signifikan antara perbedaan konsentrasi IRS-1 hati pada kelompok 3 (resisten insulin) dan kelompok 5 dan 6 (Alpha-ΜG 100, p = 1 ( > 0.05) dan Alpha-MANGOSTIN 200, p = 0.677 ( > 0.05)). Kelompok 6 memiliki konsenstrasi IRS-1 lebih tinggi dari kelompok 5, meskipun tidak secara signifikan (p = 0.558, ( > 0.05)). Kesimpulan: Pemberian alpha-mangostin 100 Mangostin dan 200 Mangostin tidak dapat meningkatkan konsentrasi IRS-1 pada hati. Kata kunci: Resistensi insulin, Alfa-mangostin, IRS-1, jaringan hati |
| id |
IOS19557.slims-28642 |
| institution |
Universitas Indonesia |
| institution_id |
51 |
| institution_type |
library:university library |
| library |
Perpustakaan FKUI |
| library_id |
7655 |
| collection |
e-Library FKUI |
| repository_id |
19557 |
| subject_area |
MESH MEDICINE_HANDBOOKS FAKULTAS KEDOKTERAN National Library Of Medicine |
| city |
JAKARTA PUSAT |
| province |
DKI JAKARTA |
| repoId |
IOS19557 |
| first_indexed |
2023-11-06T08:06:17Z |
| last_indexed |
2023-11-06T08:06:17Z |
| recordtype |
dc |
| _version_ |
1781988309638053888 |
| score |
17.607244 |