Isolation and Macroscopic Characterization of Molase and Bacteria from Acanthus sp. Mangrove Sediments
| Main Authors: | Kurniawan, Putri Nadhira Rachmani, Arifin, Zaenal |
|---|---|
| Format: | Article info application/pdf |
| Bahasa: | eng |
| Terbitan: |
CV. Hadid Mukti Karya
, 2023
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| Subjects: | |
| Online Access: |
https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1 https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1/4 |
| ctrlnum |
article-1 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en">Isolation and Macroscopic Characterization of Molase and Bacteria from Acanthus sp. Mangrove Sediments</title><creator lang="en">Kurniawan, Putri Nadhira Rachmani</creator><creator lang="en">Arifin, Zaenal</creator><subject lang="en">Bacteria</subject><subject lang="en">Mangrove Sediment</subject><subject lang="en">Molasses</subject><subject lang="en">Macroscopic Identification</subject><description lang="en">Acanthus sp. grows in mangrove sediments with a muddy texture that contains nutrients, allowing specific bacteria to thrive. This study aims to isolate and characterize bacteria from mangrove sediment (Acanthus sp.) cultured in media with added molasses. Bacteria were cultivated on solid molasses media I and II. The planting process began with the sediment sample being dissolved in distilled water for 30 minutes before undergoing sixfold dilution. Subsequently, the samples were planted on molasses media I and II with different compositions. The planting was carried out using the spread plate method, where 100µL of the final three dilutions was added. The bacteria were then incubated until bacterial colonies that could use molasses as a carbon source for their metabolism grew. Molasses is a residual substance from sugar production in sugarcane plants, which still contains fermentable sugars optimally utilized by bacteria for metabolic processes. The results of bacterial cultivation revealed differences in growth diversity, with media II exhibiting greater diversity than media I. Bacterial diversity was observed using macroscopic identification methods, focusing on the colony's appearance. This suggests that the composition of media II is more suitable for bacterial growth.</description><publisher lang="en">CV. Hadid Mukti Karya</publisher><date>2023-11-02</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>File:application/pdf</type><identifier>https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1</identifier><identifier>10.61741/wj3q0g16</identifier><source lang="en">Journal of Marine Biotechnology and Immunology; Vol. 1 No. 1 (2023): September 2023; 19-22</source><source lang="id">JOURNAL OF MARINE BIOTECHNOLOGY AND IMMUNOLOGY; Vol 1 No 1 (2023): September 2023; 19-22</source><source>3026-1457</source><source>3026-5274</source><source>10.61741/jjkdwv10</source><language>eng</language><relation>https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1/4</relation><rights lang="en">Copyright (c) 2023 Putri Nadhira Rachmani Kurniawan, Zaenal Arifin (Author)</rights><rights lang="en">https://creativecommons.org/licenses/by/4.0</rights><recordID>article-1</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File |
| author |
Kurniawan, Putri Nadhira Rachmani Arifin, Zaenal |
| title |
Isolation and Macroscopic Characterization of Molase and Bacteria from Acanthus sp. Mangrove Sediments |
| publisher |
CV. Hadid Mukti Karya |
| publishDate |
2023 |
| topic |
Bacteria Mangrove Sediment Molasses Macroscopic Identification |
| url |
https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1 https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/1/4 |
| contents |
Acanthus sp. grows in mangrove sediments with a muddy texture that contains nutrients, allowing specific bacteria to thrive. This study aims to isolate and characterize bacteria from mangrove sediment (Acanthus sp.) cultured in media with added molasses. Bacteria were cultivated on solid molasses media I and II. The planting process began with the sediment sample being dissolved in distilled water for 30 minutes before undergoing sixfold dilution. Subsequently, the samples were planted on molasses media I and II with different compositions. The planting was carried out using the spread plate method, where 100μL of the final three dilutions was added. The bacteria were then incubated until bacterial colonies that could use molasses as a carbon source for their metabolism grew. Molasses is a residual substance from sugar production in sugarcane plants, which still contains fermentable sugars optimally utilized by bacteria for metabolic processes. The results of bacterial cultivation revealed differences in growth diversity, with media II exhibiting greater diversity than media I. Bacterial diversity was observed using macroscopic identification methods, focusing on the colony's appearance. This suggests that the composition of media II is more suitable for bacterial growth. |
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natural science AQUATIC ANIMALS. -- MARINE BIOTECHNOLOGY marine immunology |
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JAWA TENGAH |
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