Susceptibility of Vibrio spp. from Gill of Barramundi (Lates calcarifer) Towards Antibiotics

Main Authors: Heriyati, Eny, Larasati, Prita Lintang
Format: Article info application/pdf
Bahasa: eng
Terbitan: CV. Hadid Mukti Karya , 2023
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Online Access: https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2
https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2/5
ctrlnum article-2
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en">Susceptibility of Vibrio spp. from Gill of Barramundi (Lates calcarifer) Towards Antibiotics</title><creator lang="en">Heriyati, Eny</creator><creator lang="en">Larasati, Prita Lintang</creator><subject lang="en">Antibiotics</subject><subject lang="en">Sensitivity</subject><subject lang="en">Susceptibility</subject><subject lang="en">Vibrio spp.</subject><description lang="en">Vibrio spp. bacteria are aquatic bacteria that can be found in brackish water and estuaries, and they have opportunistic pathogenic properties. These bacteria are usually dominant in brackish and estuarine areas such as river mouths, rivers, ponds, and seas with salinities between 20-40 ppt and a temperature of 37&#xB0;C. The aim of this research is to determine the susceptibility and sensitivity of Vibrio spp. bacteria to various types of antibiotics. The Vibrio spp. bacteria isolated in this study were obtained from barramundi fish (Lates calcarifer). If they are still sensitive, the growth of Vibrio spp. bacteria can be inhibited by antibiotic administration. The effectiveness of antibiotics against Vibrio spp. bacteria in barramundi fish was tested using eight types of antibiotics, namely Co-Amoxiclav, Gentamicin, Doxycycline, Tetracycline, Azithromycin, Chloramphenicol, Ciprofloxacin, and Ampicillin. Bacteria that are sensitive to specific types of antibiotics have larger inhibition zones. The measurement of inhibition zones was carried out using a caliper by measuring the vertical and horizontal diameters and then dividing by 2 to obtain the average. This average was compared to a standard interpretative zone diameter chart to determine antibiotic sensitivity and resistance status using the Disk Diffusion method. Tetracycline and Ampicillin antibiotics can inhibit or are resistant to Vibrio spp. bacteria with a percentage of 0.08%. Gentamicin has greater sensitivity compared to the other tested antibiotics. In this study, the Vibrio spp. obtained are still sensitive to certain types of antibiotics and resistant to Ampicillin.</description><publisher lang="en">CV. Hadid Mukti Karya</publisher><date>2023-11-02</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>File:application/pdf</type><identifier>https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2</identifier><identifier>10.61741/hb3cm374</identifier><source lang="en">Journal of Marine Biotechnology and Immunology; Vol. 1 No. 1 (2023): September 2023; 23-30</source><source lang="id">JOURNAL OF MARINE BIOTECHNOLOGY AND IMMUNOLOGY; Vol 1 No 1 (2023): September 2023; 23-30</source><source>3026-1457</source><source>3026-5274</source><source>10.61741/jjkdwv10</source><language>eng</language><relation>https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2/5</relation><rights lang="en">Copyright (c) 2023 Eny Heriyati, Prita Lintang Larasati (Author)</rights><rights lang="en">https://creativecommons.org/licenses/by/4.0</rights><recordID>article-2</recordID></dc>
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author Heriyati, Eny
Larasati, Prita Lintang
title Susceptibility of Vibrio spp. from Gill of Barramundi (Lates calcarifer) Towards Antibiotics
publisher CV. Hadid Mukti Karya
publishDate 2023
topic Antibiotics
Sensitivity
Susceptibility
Vibrio spp
url https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2
https://ejournal.immunolmarbiotech.com/index.php/JMBI/article/view/2/5
contents Vibrio spp. bacteria are aquatic bacteria that can be found in brackish water and estuaries, and they have opportunistic pathogenic properties. These bacteria are usually dominant in brackish and estuarine areas such as river mouths, rivers, ponds, and seas with salinities between 20-40 ppt and a temperature of 37°C. The aim of this research is to determine the susceptibility and sensitivity of Vibrio spp. bacteria to various types of antibiotics. The Vibrio spp. bacteria isolated in this study were obtained from barramundi fish (Lates calcarifer). If they are still sensitive, the growth of Vibrio spp. bacteria can be inhibited by antibiotic administration. The effectiveness of antibiotics against Vibrio spp. bacteria in barramundi fish was tested using eight types of antibiotics, namely Co-Amoxiclav, Gentamicin, Doxycycline, Tetracycline, Azithromycin, Chloramphenicol, Ciprofloxacin, and Ampicillin. Bacteria that are sensitive to specific types of antibiotics have larger inhibition zones. The measurement of inhibition zones was carried out using a caliper by measuring the vertical and horizontal diameters and then dividing by 2 to obtain the average. This average was compared to a standard interpretative zone diameter chart to determine antibiotic sensitivity and resistance status using the Disk Diffusion method. Tetracycline and Ampicillin antibiotics can inhibit or are resistant to Vibrio spp. bacteria with a percentage of 0.08%. Gentamicin has greater sensitivity compared to the other tested antibiotics. In this study, the Vibrio spp. obtained are still sensitive to certain types of antibiotics and resistant to Ampicillin.
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AQUATIC ANIMALS. -- MARINE BIOTECHNOLOGY
marine immunology
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