Tampilan Petugas: IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)

IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)

Main Authors:	Sarry Hartono, Teguh, Murniati, Dewi, Yasmon, Andi, H Moehario, Lucky
Format:	Article info application/pdf eJournal
Bahasa:	eng
Terbitan:	Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso , 2017
Subjects:	Gut microbiota diarrhea PCR ESI-MS
Online Access:	https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21 https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21/20

ctrlnum	article-21
fullrecord	<?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)</title><creator>Sarry Hartono, Teguh</creator><creator>Murniati, Dewi</creator><creator>Yasmon, Andi</creator><creator>H Moehario, Lucky</creator><subject lang="en-US">Gut microbiota</subject><subject lang="en-US">diarrhea</subject><subject lang="en-US">PCR/ESI-MS</subject><description lang="en-US">Abstract :Microbiota present in human intestinal are diverse, and imbalance in composition of intestinal flora may cause diarrhea.This study aimed to obtain a profile of intestinal bacteria in children with and without diarrhea and assess their presence with incidence of diarrhea. An analitical descriptive with cross sectional design study was carried out. A stool specimen was collected from each children of 2-12 years old with and without diarrhea who lived in North Jakarta. DNA extraction was performed prior to detection of microbes using Polymerase Chain Ceaction/Electrospray Ionization-Mass Spectrometry.Eighty stool specimens consisted of 33 and 47 specimens from children with and without diarrhea were included in the study. Thirty single and 6 multiple matches were detected in 30 specimens of the diarrhea group; 28 single and 8 multiple matches were found in 34 specimens of the non-diarrhea.Escherechiacoli and Klebsiella pneumonia were predominant in both groups. Firmicutes, Proteobacteria and Bacteroidetes were deteced in the diarrhea group, while Actinobacteria, Proteobacteria and Verrucomicrobia were in the non-diarrhea. The relationship of incidence of diarrhea and the present of enteropathogens in the stool was not significant, however, there was a strong correlation of the risk of suffering diarrhea due to the presence of enteropathogens (OR = 0.724 with 95%, CI: 0.237-2.215).In conclusion, most bacteria detected in both groups were similar, nonetheless, Actinobacteria was present only in the non-diarrhea. The chance to have diarrhea was higher when enteropathogen was detected in the stool.</description><publisher lang="en-US">Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso</publisher><date>2017-11-05</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21</identifier><identifier>10.32667/ijid.v2i2.21</identifier><source lang="en-US">The Indonesian Journal of Infectious Diseases; Vol. 2 No. 2 (2015): THE INDONESIAN JOURNAL OF INFECTIOUS DISEASES; 1-9</source><source>2599-1698</source><source>2354-6077</source><source>10.32667/ijid.v2i2</source><language>eng</language><relation>https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21/20</relation><rights lang="en-US">Copyright (c) 2017 The Indonesian Journal of Infectious Diseases</rights><recordID>article-21</recordID></dc>
language	eng
format	Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal
author	Sarry Hartono, Teguh Murniati, Dewi Yasmon, Andi H Moehario, Lucky
title	IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)
publisher	Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso
publishDate	2017
topic	Gut microbiota diarrhea PCR ESI-MS
url	https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21 https://www.ijid-rspisuliantisaroso.co.id/index.php/ijid/article/view/21/20
contents	Abstract :Microbiota present in human intestinal are diverse, and imbalance in composition of intestinal flora may cause diarrhea.This study aimed to obtain a profile of intestinal bacteria in children with and without diarrhea and assess their presence with incidence of diarrhea. An analitical descriptive with cross sectional design study was carried out. A stool specimen was collected from each children of 2-12 years old with and without diarrhea who lived in North Jakarta. DNA extraction was performed prior to detection of microbes using Polymerase Chain Ceaction/Electrospray Ionization-Mass Spectrometry.Eighty stool specimens consisted of 33 and 47 specimens from children with and without diarrhea were included in the study. Thirty single and 6 multiple matches were detected in 30 specimens of the diarrhea group; 28 single and 8 multiple matches were found in 34 specimens of the non-diarrhea.Escherechiacoli and Klebsiella pneumonia were predominant in both groups. Firmicutes, Proteobacteria and Bacteroidetes were deteced in the diarrhea group, while Actinobacteria, Proteobacteria and Verrucomicrobia were in the non-diarrhea. The relationship of incidence of diarrhea and the present of enteropathogens in the stool was not significant, however, there was a strong correlation of the risk of suffering diarrhea due to the presence of enteropathogens (OR = 0.724 with 95%, CI: 0.237-2.215).In conclusion, most bacteria detected in both groups were similar, nonetheless, Actinobacteria was present only in the non-diarrhea. The chance to have diarrhea was higher when enteropathogen was detected in the stool.
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library_id	8009
collection	The Indonesian Journal of Infectious Disease
repository_id	20143
subject_area	Kesehatan masyarakat Penyakit Infeksi
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IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)

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