Pengembangan Metode Ekstraksi dan Analisis Irbesartan dalam Plasma Darah menggunakan High Performance Liquid Chromatography (HPLC) Fluorescence
| Main Author: | ., Ratih |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
ISFI
, 2015
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/26515/7/Ratih_Pengembangan%20Metode_Abstrak_2015.pdf http://repository.ubaya.ac.id/26515/8/Ratih_Pengembangan%20Metode_2015.pdf http://repository.ubaya.ac.id/26515/ |
Daftar Isi:
- A simple extraction and analysis method by High Performance Liquid Chromatography (HPLC) Fluorescence has developed for determination of Irbesartan in plasma sample. The chromatography system were Fluorescence detector at λ ex254nm/em380 nm, Flow rate 1 mL/min, C-18 150 x 4,6 mm; 5μm column and acetonitrile : 0,5% (v/v) H3PO4 pH 3,0 (77:23) as mobile phase by run time not more than 6 minutes. Reliability of the HPLC method was assessed by the linearity of 9 levels concentration which be found 10.0 – 5,000.0 ng/mL, r = 0.9996 respectively. Chloroform was used as pretreatment solvent to extract Irbesartan dan Candesartan cilexetil (Internal Standard) from protein binding. The chromatogram of Irbesartan dan Candesartan cilexetil peaks were clearly separated without matrix noises, it was showed by resolution factor > 2.