Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp

Main Authors: Hanim, Chusnul, Yusiati, Lies Mira, Jazim, Fahriza Anjaya
Format: Proceeding PeerReviewed application/pdf
Bahasa: eng
Terbitan: , 2015
Subjects:
Online Access: https://repository.ugm.ac.id/273330/1/ISTAP%20%20Bu%20Hanim.pdf
https://repository.ugm.ac.id/273330/
ctrlnum 273330
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/273330/</relation><title>Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas&#xD; Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.</title><creator>Hanim, Chusnul</creator><creator>Yusiati, Lies Mira</creator><creator>Jazim, Fahriza Anjaya</creator><subject>Crop and Pasture Production</subject><subject>Crop and Pasture Post Harvest Technologies (incl. Transportation and Storage)</subject><description>The experiment was done to evaluate the influence of robusta coffee pulp fermented&#xD; by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was&#xD; cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The&#xD; inoculum produced was then used for coffee pulp fermentation. Cellulolytic bacteria was added&#xD; into 200 g coffee pulp as much as 0%, 5% and 10% based on dry matter. Each treatment had three&#xD; replicates. Fermentation was carried out at room temperature during 21 days anaerobically. At&#xD; the end of fermentation, samples were taken out for nutrient determination including physical,&#xD; chemical qualities, and in vitro gas production. Data obtained were analyzed by one way design&#xD; and continued by Duncan&#x2019;s new multiple range test to examine the differences between mean&#xD; values. The results showed that 5% and 10% cellulolytic bacteria addition decreased pH value, and&#xD; crude fiber (CF) content as much as 12.89% and 16.32% compared to control (0% of cellulolytic&#xD; bacteria). Whereas addition of 10% inoculum increased nitrogen free extract (NFE) content.&#xD; However, cellulolytic bacteria addition up to 10% had no effect on crude protein (CP), extract&#xD; ether (EE), dry matter (DM), organic matter (OM), glucose, and lactic acid content, as well as in&#xD; vitro gas production. It could be concluded that cellulolytic bacteria addition in level 5% decreased&#xD; CF content but did not give positive effect on in vitro gas production.</description><date>2015-10-22</date><type>Journal:Proceeding</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/273330/1/ISTAP%20%20Bu%20Hanim.pdf</identifier><identifier> Hanim, Chusnul and Yusiati, Lies Mira and Jazim, Fahriza Anjaya (2015) Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp. In: The 6th ISTAPInternational Seminar on Tropical Animal Production, 20-22 Oktober 2015, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, Indonesia. </identifier><recordID>273330</recordID></dc>
language eng
format Journal:Proceeding
Journal
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Hanim, Chusnul
Yusiati, Lies Mira
Jazim, Fahriza Anjaya
title Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp
publishDate 2015
topic Crop and Pasture Production
Crop and Pasture Post Harvest Technologies (incl. Transportation and Storage)
url https://repository.ugm.ac.id/273330/1/ISTAP%20%20Bu%20Hanim.pdf
https://repository.ugm.ac.id/273330/
contents The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The inoculum produced was then used for coffee pulp fermentation. Cellulolytic bacteria was added into 200 g coffee pulp as much as 0%, 5% and 10% based on dry matter. Each treatment had three replicates. Fermentation was carried out at room temperature during 21 days anaerobically. At the end of fermentation, samples were taken out for nutrient determination including physical, chemical qualities, and in vitro gas production. Data obtained were analyzed by one way design and continued by Duncan’s new multiple range test to examine the differences between mean values. The results showed that 5% and 10% cellulolytic bacteria addition decreased pH value, and crude fiber (CF) content as much as 12.89% and 16.32% compared to control (0% of cellulolytic bacteria). Whereas addition of 10% inoculum increased nitrogen free extract (NFE) content. However, cellulolytic bacteria addition up to 10% had no effect on crude protein (CP), extract ether (EE), dry matter (DM), organic matter (OM), glucose, and lactic acid content, as well as in vitro gas production. It could be concluded that cellulolytic bacteria addition in level 5% decreased CF content but did not give positive effect on in vitro gas production.
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