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ANTI-AGING EFFECTS OF MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IN MICE SKIN MODEL INDUCED BY D-GALACTOSE

Main Authors: Irma Padeta, IP, Armanda Dwi Prayugo, ADP, Widagdo Sri Nugroho, WSN, Teguh Budipitojo, TB
Format: Article PeerReviewed application/pdf
Bahasa: eng
Terbitan: Faculty of Veterinary Medicine, Universitas Gadjah Mada , 2017
Subjects:
Veterinary Medicine
Online Access: https://repository.ugm.ac.id/274657/1/Padeta%20et%20al%202017.pdf
https://repository.ugm.ac.id/274657/
ctrlnum 274657
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/274657/</relation><title>ANTI-AGING EFFECTS OF MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IN MICE SKIN MODEL INDUCED BY D-GALACTOSE</title><creator>Irma Padeta, IP</creator><creator>Armanda Dwi Prayugo, ADP</creator><creator>Widagdo Sri Nugroho, WSN</creator><creator>Teguh Budipitojo, TB</creator><subject>Veterinary Medicine</subject><description>Skin aging is a degenerative process influenced by intrinsic and extrinsic factors. Mesenchymal stem cell-conditioned medium (MSC-CM) known as secretome, contains a lot of regenerative molecules known as cytokines and growth factors. The objective of this study was to investigate the anti-aging effect of MSC-CM in mice skin model induced by D-galactose. Thirty-nine of 6 weeks old mice were divided into 4 group, control group, D-galactose (D-gal) group, saline treated group and MSC-CM treated group. D-galactose were injected once a week for 8 weeks to induce skin aging at the dose rate of 1000 mg/kg BW. After skin aging induction, mice were injected subcutaneously with saline in saline treated group and MSC-CM in MSC-CM treated group once a week. Mice were sacrificed before D-gal induction, after D-gal induction and after treated by saline and MSC-CM. Skin tissues were collected and processed by paraffin method. Sample tissues were stained with Hematoxyline-Eosin and Mallory Aniline Blue staining method. MSC-CM recovered the skin structure and morphology as indicated by the enhance of epidermal and dermal thickness, enhance vascularization and hair follicle formation, and very high density of collagen fibers deposition. Subcutaneous injection of MSC-CM promoted the regeneration of mice skin aging induced by D-galactose.</description><publisher>Faculty of Veterinary Medicine, Universitas Gadjah Mada</publisher><date>2017-07-22</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/274657/1/Padeta%20et%20al%202017.pdf</identifier><identifier> Irma Padeta, IP and Armanda Dwi Prayugo, ADP and Widagdo Sri Nugroho, WSN and Teguh Budipitojo, TB (2017) ANTI-AGING EFFECTS OF MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IN MICE SKIN MODEL INDUCED BY D-GALACTOSE. PROCEEDING :One Health Approach to Control ZoonoticDisease and Improve Quality Life. pp. 104-111. </identifier><recordID>274657</recordID></dc>
language eng
format Journal:Article
Journal
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Irma Padeta, IP
Armanda Dwi Prayugo, ADP
Widagdo Sri Nugroho, WSN
Teguh Budipitojo, TB
title ANTI-AGING EFFECTS OF MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IN MICE SKIN MODEL INDUCED BY D-GALACTOSE
publisher Faculty of Veterinary Medicine, Universitas Gadjah Mada
publishDate 2017
topic Veterinary Medicine
url https://repository.ugm.ac.id/274657/1/Padeta%20et%20al%202017.pdf
https://repository.ugm.ac.id/274657/
contents Skin aging is a degenerative process influenced by intrinsic and extrinsic factors. Mesenchymal stem cell-conditioned medium (MSC-CM) known as secretome, contains a lot of regenerative molecules known as cytokines and growth factors. The objective of this study was to investigate the anti-aging effect of MSC-CM in mice skin model induced by D-galactose. Thirty-nine of 6 weeks old mice were divided into 4 group, control group, D-galactose (D-gal) group, saline treated group and MSC-CM treated group. D-galactose were injected once a week for 8 weeks to induce skin aging at the dose rate of 1000 mg/kg BW. After skin aging induction, mice were injected subcutaneously with saline in saline treated group and MSC-CM in MSC-CM treated group once a week. Mice were sacrificed before D-gal induction, after D-gal induction and after treated by saline and MSC-CM. Skin tissues were collected and processed by paraffin method. Sample tissues were stained with Hematoxyline-Eosin and Mallory Aniline Blue staining method. MSC-CM recovered the skin structure and morphology as indicated by the enhance of epidermal and dermal thickness, enhance vascularization and hair follicle formation, and very high density of collagen fibers deposition. Subcutaneous injection of MSC-CM promoted the regeneration of mice skin aging induced by D-galactose.
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first_indexed 2019-05-05T02:53:35Z
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