PENGARUH KETAMIN INTRAVENA TERHADAP KADAR NITRIC OXIDE MAKROFAG MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA THE EFFECT OF INTRAVENOUS KETAMINE ON MACROPHAGE NITRIC OXIDE LEVEL IN BALB/C MICE WITH LIPOPOLYSACCHARIDE INDUCED
Main Author: | Prastyo H, Sulung |
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Format: | Thesis NonPeerReviewed application/pdf |
Terbitan: |
, 2009
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Subjects: | |
Online Access: |
http://eprints.undip.ac.id/24722/1/Sulung_Prastyo_H.pdf http://eprints.undip.ac.id/24722/ |
ctrlnum |
24722 |
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fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>PENGARUH KETAMIN INTRAVENA
TERHADAP KADAR NITRIC OXIDE MAKROFAG
MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA
THE EFFECT OF INTRAVENOUS KETAMINE
ON MACROPHAGE NITRIC OXIDE LEVEL IN BALB/C MICE
WITH LIPOPOLYSACCHARIDE INDUCED</title><creator>Prastyo H, Sulung </creator><subject>R Medicine (General)</subject><description>PENGARUH KETAMIN INTRAVENA
TERHADAP KADAR NITRIC OXIDE MAKROFAG
MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA
Latar belakang: nitric oxide (NO) berperan dalam patogenesis terjadinya hipotensi
sistemik pada syok septic. Paparan endotoksin akan menyebabkan peningkatan
pelepasan NO yang dipengaruhi oleh aktivasi sitokin proinflamasi. Ketamin
merupakan obat anestesi yang digunakan untuk penderita sepsis, diduga menekan
produksi sitokin proinflamasi akibat paparan lipopolisakarida (LPS), sehingga
pembentukan NO dapat dihambat.
Tujuan: Menilai pengaruh pemberian ketamin dosis 0,1;0,2 dan 0,4 mg
intravena terhadap kadar NO mencit yang diberi lipopolisakarida
intraperitoneal.
Metode: Penelitian eksperimental laboratorik dengan desain randomized post test
only controlled group pada 20 ekor mencit Balb/c yang disuntik lipoplisakarida
intraperitoneal dan Ketamin dosis 0,1 ; 0,2 dan 0,4 mg intravena. Mencit dibagi
menjadi 4 kelompok secara random, yaitu K1 sebagai kontrol, K2 yang mendapat
ketamin 0,1 mg, K3 yang mendapat ketamin 0,2 mg, dan K4 yang mendapat ketamin
0,4 mg. Pemeriksaan NO diambil dari kultur makrofag intraperitoneal setelah 6 jam
pemberian ketamin. Uji statistik yang digunakan adalah parametrik ANOVA dan
dilanjutkan Post hoc dengan derajat kemaknaan p<0,05.
Hasil: Kadar rerata NO pada kelompok K1(43,2±2,2),K2(14,8±1,2), K3(11,6±2) dan
K4(10,7±1,7). Terdapat perbedaan kadar NO yang signifikan pada kelompok K2,K3
dan K4 dibanding K1 dengan p<0,001, K2 dibanding K3 dengan p=0,015 serta K2
dibanding K4 dengan p=0,002. Sedangkan antara kelompok K3 dibanding K4 tidak
didapatkan perbedaan yang bermakna dengan p=0,402.
Simpulan: Ketamin dapat menurunkan kadar NO makrofag intraperitoneal mencit
secara signifikan. Ketamin dosis 0,2 mg intravena efektif untuk menurunkan kadar
NO makrofag intraperitoneal.
Kata kunci: lipopolisakarida, ketamin, nitric oxide (NO).
ABSTRACT
THE EFFECT OF INTRAVENOUS KETAMINE ON MACROPHAGE NITRIC
OXIDE LEVEL IN BALB/C MICE WITH LIPOPOLYSACCHARIDE
INDUCED
Background: Nitric oxide (NO) has a potential rule in pathogenesis of systemic
hypotension in sepsis. Endotoxin increases the activity of the iNOS enzyme and NO
release. Proinflamatory cytokines is inflammatory mediators implicated in the
induction and activation of iNOS and NO release also. Ketamine is an anesthetic
agent and commonly used for septic patients. This agent suppresses endotoxin
induced proinflamatory cytokines production and blockade the activation of NF-kB,
so the NO production can be inhibited.
Objective: to study the effect of Ketamine 0,1;0,2 and 0,4 mg intravenous on
intraperitoneal NO level on Balb/c mice with intraperitoneal injection of
lipopolysaccharide.
Methods: a randomized post test only controlled group laboratoric experimental
studied on 20 male Balb/c mice divided into 4 groups and injected intraperitoneally
with lipopolysaccharide 20mg/kg and 6 hours later were injected with ketamine . K1
as the control group ; K2, K3, and K4 administrered with ketamine 0,1;0,2; and 0,4
mg intravenously. NO was taken from peritoneal macrophage culture and observed
by Grease method. The results were analyzed by ANOVA and post hoc statistical
assays, with reliability p < 0,05.
Results: NO level K1(43,2±2,2),K2(14,8±1,2), K3(11,6±2) and K4(10,7±1,7). There
were significant difference in NO level between K2,K3 and K4 than K1 (p<0,001), K2
than K3 (p=0,015) and K2 than K4 (p=0,002). But there was no significant difference
between K3 and K4 (p=0,402).
Conclusion: Ketamine decreases nitric oxide level in mice intraperitoneal
macrophages significantly. Ketamine 0,2 mg is the effective dose for decreasing
intraperitoneal nitric oxide level.
Keywords: ketamine, lipopolysaccharide, nitric oxide.</description><date>2009</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><type>File:application/pdf</type><identifier>http://eprints.undip.ac.id/24722/1/Sulung_Prastyo_H.pdf</identifier><identifier>Prastyo H, Sulung (2009) PENGARUH KETAMIN INTRAVENA TERHADAP KADAR NITRIC OXIDE MAKROFAG MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA THE EFFECT OF INTRAVENOUS KETAMINE ON MACROPHAGE NITRIC OXIDE LEVEL IN BALB/C MICE WITH LIPOPOLYSACCHARIDE INDUCED. Masters thesis, UNIVERSITAS DIPONEGORO.</identifier><relation>http://eprints.undip.ac.id/24722/</relation><recordID>24722</recordID></dc>
|
format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview File:application/pdf File |
author |
Prastyo H, Sulung |
title |
PENGARUH KETAMIN INTRAVENA
TERHADAP KADAR NITRIC OXIDE MAKROFAG
MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA
THE EFFECT OF INTRAVENOUS KETAMINE
ON MACROPHAGE NITRIC OXIDE LEVEL IN BALB/C MICE
WITH LIPOPOLYSACCHARIDE INDUCED |
publishDate |
2009 |
topic |
R Medicine (General) |
url |
http://eprints.undip.ac.id/24722/1/Sulung_Prastyo_H.pdf http://eprints.undip.ac.id/24722/ |
contents |
PENGARUH KETAMIN INTRAVENA
TERHADAP KADAR NITRIC OXIDE MAKROFAG
MENCIT BALB/C YANG DIBERI LIPOPOLISAKARIDA
Latar belakang: nitric oxide (NO) berperan dalam patogenesis terjadinya hipotensi
sistemik pada syok septic. Paparan endotoksin akan menyebabkan peningkatan
pelepasan NO yang dipengaruhi oleh aktivasi sitokin proinflamasi. Ketamin
merupakan obat anestesi yang digunakan untuk penderita sepsis, diduga menekan
produksi sitokin proinflamasi akibat paparan lipopolisakarida (LPS), sehingga
pembentukan NO dapat dihambat.
Tujuan: Menilai pengaruh pemberian ketamin dosis 0,1;0,2 dan 0,4 mg
intravena terhadap kadar NO mencit yang diberi lipopolisakarida
intraperitoneal.
Metode: Penelitian eksperimental laboratorik dengan desain randomized post test
only controlled group pada 20 ekor mencit Balb/c yang disuntik lipoplisakarida
intraperitoneal dan Ketamin dosis 0,1 ; 0,2 dan 0,4 mg intravena. Mencit dibagi
menjadi 4 kelompok secara random, yaitu K1 sebagai kontrol, K2 yang mendapat
ketamin 0,1 mg, K3 yang mendapat ketamin 0,2 mg, dan K4 yang mendapat ketamin
0,4 mg. Pemeriksaan NO diambil dari kultur makrofag intraperitoneal setelah 6 jam
pemberian ketamin. Uji statistik yang digunakan adalah parametrik ANOVA dan
dilanjutkan Post hoc dengan derajat kemaknaan p<0,05.
Hasil: Kadar rerata NO pada kelompok K1(43,2±2,2),K2(14,8±1,2), K3(11,6±2) dan
K4(10,7±1,7). Terdapat perbedaan kadar NO yang signifikan pada kelompok K2,K3
dan K4 dibanding K1 dengan p<0,001, K2 dibanding K3 dengan p=0,015 serta K2
dibanding K4 dengan p=0,002. Sedangkan antara kelompok K3 dibanding K4 tidak
didapatkan perbedaan yang bermakna dengan p=0,402.
Simpulan: Ketamin dapat menurunkan kadar NO makrofag intraperitoneal mencit
secara signifikan. Ketamin dosis 0,2 mg intravena efektif untuk menurunkan kadar
NO makrofag intraperitoneal.
Kata kunci: lipopolisakarida, ketamin, nitric oxide (NO).
ABSTRACT
THE EFFECT OF INTRAVENOUS KETAMINE ON MACROPHAGE NITRIC
OXIDE LEVEL IN BALB/C MICE WITH LIPOPOLYSACCHARIDE
INDUCED
Background: Nitric oxide (NO) has a potential rule in pathogenesis of systemic
hypotension in sepsis. Endotoxin increases the activity of the iNOS enzyme and NO
release. Proinflamatory cytokines is inflammatory mediators implicated in the
induction and activation of iNOS and NO release also. Ketamine is an anesthetic
agent and commonly used for septic patients. This agent suppresses endotoxin
induced proinflamatory cytokines production and blockade the activation of NF-kB,
so the NO production can be inhibited.
Objective: to study the effect of Ketamine 0,1;0,2 and 0,4 mg intravenous on
intraperitoneal NO level on Balb/c mice with intraperitoneal injection of
lipopolysaccharide.
Methods: a randomized post test only controlled group laboratoric experimental
studied on 20 male Balb/c mice divided into 4 groups and injected intraperitoneally
with lipopolysaccharide 20mg/kg and 6 hours later were injected with ketamine . K1
as the control group ; K2, K3, and K4 administrered with ketamine 0,1;0,2; and 0,4
mg intravenously. NO was taken from peritoneal macrophage culture and observed
by Grease method. The results were analyzed by ANOVA and post hoc statistical
assays, with reliability p < 0,05.
Results: NO level K1(43,2±2,2),K2(14,8±1,2), K3(11,6±2) and K4(10,7±1,7). There
were significant difference in NO level between K2,K3 and K4 than K1 (p<0,001), K2
than K3 (p=0,015) and K2 than K4 (p=0,002). But there was no significant difference
between K3 and K4 (p=0,402).
Conclusion: Ketamine decreases nitric oxide level in mice intraperitoneal
macrophages significantly. Ketamine 0,2 mg is the effective dose for decreasing
intraperitoneal nitric oxide level.
Keywords: ketamine, lipopolysaccharide, nitric oxide. |
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