The Effect Of Osteopontin In Semen Freezing Process On The Quality Of Holstein Friesian Dairy Bull Frozen Semen
| Main Author: | Hernawati, Tatik |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Asian Academic Research Journal
, 2015
|
| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/84602/3/Bukti%2001.%20The%20Effect%20of%20Osteopontin%20in%20.....-min.pdf http://repository.unair.ac.id/84602/2/Peer%20Review%20Bukti%2001.pdf http://repository.unair.ac.id/84602/4/BUKTI%201%20THE%20EFFECT%20OF%20OSTEOPONTIN%20IN%20SEMEN%20FREEZING%20PROCESS%20ON%20THE%20QUALITY%20OF%20HOLSTEIN%20FRIESIAN%20DAIRY%20BULL%20FROZEN%20SEMEN.-compressed.pdf http://repository.unair.ac.id/84602/ https://www.asianacademicresearch.org/2015_abstract/may_md_2015/10.pdf |
| ctrlnum |
84602 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.unair.ac.id/84602/</relation><title>The Effect Of Osteopontin In Semen Freezing Process On The Quality Of Holstein Friesian Dairy Bull Frozen Semen</title><creator>Hernawati, Tatik</creator><subject>SF600-1100 Veterinary medicine</subject><description>Background : The quality of frozen semen after thawing is one of factors in artificial
insemination. Cryopreservation affects the post-thawed sperm quality. The seminal plasma
diary bull’s contains high concentration of osteopontin. Our hypothesis is that osteopontin
maintain sperm quality during cryopreservation.
Aim : The first aim was isolation, identification and protein specification of osteopontin
from dairy bull semen. The second aim was to investigate osteopontin does improve the
quality of frozen semen as appears from increased expression of HSP 70 drecreased apoptotic
expression and increased viability and motility.
Method : isolation, identification and protein specification of osteopontin from dairy bull
semen. using SDS-PAGE method indicated that there were some tapes showing different
molecular weight from several samples. SDS-PAGE result from protein identification was
continued with protein identification using Western Blot technique. The effect of osteopontin
on the quality of frozen semen post thawing by the expression of HSP 70 with
immunocytochemistry techniques. The expression of apoptotic spermatozoa by Tunnel
Assay. The viability spermatozoa using negrosin eosin staining and by assesing motility of
spermatozoa with direct microscopy.
Result of the first phase was determined that molecular weight of osteopontin is 56 kDa.
Osteopontin concentration from electroelution samples were analyzed using Nano drop
method, and it showed the protein concentrations as avaregely 180 μg /ml. result of the
second phase indicated that the addition of osteopontin can improve semen quality through
the increase of HSP70 resulting in a decrease in apopotosis. The result of all causes
increased motility mechanisms, either directly or indirectly based on viability.
Conclusion : Addition of osteopontin may improve semen quality through increased HSP70
resulting in a decrease in viability and motility of apoptosis that can be maintained.
Key words: Osteopontin, Friesian Holstein diary bull, seminal plasma, post- thawed sperm
quality.</description><publisher>Asian Academic Research Journal</publisher><date>2015-05</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84602/3/Bukti%2001.%20The%20Effect%20of%20Osteopontin%20in%20.....-min.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84602/2/Peer%20Review%20Bukti%2001.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84602/4/BUKTI%201%20THE%20EFFECT%20OF%20OSTEOPONTIN%20IN%20SEMEN%20FREEZING%20PROCESS%20ON%20THE%20QUALITY%20OF%20HOLSTEIN%20FRIESIAN%20DAIRY%20BULL%20FROZEN%20SEMEN.-compressed.pdf</identifier><identifier> Hernawati, Tatik (2015) The Effect Of Osteopontin In Semen Freezing Process On The Quality Of Holstein Friesian Dairy Bull Frozen Semen. Asian Academic Research Journal of Multidisciplinary, 1 (33). pp. 120-132. ISSN 2319-2801 </identifier><relation>https://www.asianacademicresearch.org/2015_abstract/may_md_2015/10.pdf</relation><recordID>84602</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal PeerReview:PeerReviewed PeerReview Book:Book Book |
| author |
Hernawati, Tatik |
| title |
The Effect Of Osteopontin In Semen Freezing Process On The Quality Of Holstein Friesian Dairy Bull Frozen Semen |
| publisher |
Asian Academic Research Journal |
| publishDate |
2015 |
| topic |
SF600-1100 Veterinary medicine |
| url |
http://repository.unair.ac.id/84602/3/Bukti%2001.%20The%20Effect%20of%20Osteopontin%20in%20.....-min.pdf http://repository.unair.ac.id/84602/2/Peer%20Review%20Bukti%2001.pdf http://repository.unair.ac.id/84602/4/BUKTI%201%20THE%20EFFECT%20OF%20OSTEOPONTIN%20IN%20SEMEN%20FREEZING%20PROCESS%20ON%20THE%20QUALITY%20OF%20HOLSTEIN%20FRIESIAN%20DAIRY%20BULL%20FROZEN%20SEMEN.-compressed.pdf http://repository.unair.ac.id/84602/ https://www.asianacademicresearch.org/2015_abstract/may_md_2015/10.pdf |
| contents |
Background : The quality of frozen semen after thawing is one of factors in artificial
insemination. Cryopreservation affects the post-thawed sperm quality. The seminal plasma
diary bull’s contains high concentration of osteopontin. Our hypothesis is that osteopontin
maintain sperm quality during cryopreservation.
Aim : The first aim was isolation, identification and protein specification of osteopontin
from dairy bull semen. The second aim was to investigate osteopontin does improve the
quality of frozen semen as appears from increased expression of HSP 70 drecreased apoptotic
expression and increased viability and motility.
Method : isolation, identification and protein specification of osteopontin from dairy bull
semen. using SDS-PAGE method indicated that there were some tapes showing different
molecular weight from several samples. SDS-PAGE result from protein identification was
continued with protein identification using Western Blot technique. The effect of osteopontin
on the quality of frozen semen post thawing by the expression of HSP 70 with
immunocytochemistry techniques. The expression of apoptotic spermatozoa by Tunnel
Assay. The viability spermatozoa using negrosin eosin staining and by assesing motility of
spermatozoa with direct microscopy.
Result of the first phase was determined that molecular weight of osteopontin is 56 kDa.
Osteopontin concentration from electroelution samples were analyzed using Nano drop
method, and it showed the protein concentrations as avaregely 180 μg /ml. result of the
second phase indicated that the addition of osteopontin can improve semen quality through
the increase of HSP70 resulting in a decrease in apopotosis. The result of all causes
increased motility mechanisms, either directly or indirectly based on viability.
Conclusion : Addition of osteopontin may improve semen quality through increased HSP70
resulting in a decrease in viability and motility of apoptosis that can be maintained.
Key words: Osteopontin, Friesian Holstein diary bull, seminal plasma, post- thawed sperm
quality. |
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Universitas Airlangga |
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Adat Istiadat Administrasi Negara dan Militer Agama |
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JAWA TIMUR |
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