Supplementation of Specific Proteins in The Seminal Plasma Frozen Semen Diluter Mediaon The Plasma Membrane Intact, The Expression of Caspase and Malondialdehyde Post Thawing
| Main Author: | Hernawati, Tatik |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Asian Academic Research Journal Associates
, 2016
|
| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/84618/1/Bukti%2003.%20Supplementation%20of%20Specific%20......pdf http://repository.unair.ac.id/84618/2/Peer%20Review%20Bukti%2003.pdf http://repository.unair.ac.id/84618/3/BUKTI%203%20SUPPLEMENTATION%20OF%20SPECIFIC%20PROTEINS%20IN%20THE%20SEMINAL%20PLASMA%20FROZEN%20SEMEN%20DILUTER%20MEDIAON%20THE%20PLASMA%20MEMBRANE%20INTACT%2C%20THE%20EXPRESSION%20OF%20CASPASE%20AND%20MALONDIALDEHYDE%20POST%20THAWING-compressed.pdf http://repository.unair.ac.id/84618/ https://www.asianacademicresearch.org/2016_abstract/april_md_2016/12.pdf |
| ctrlnum |
84618 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.unair.ac.id/84618/</relation><title>Supplementation of Specific Proteins in The Seminal Plasma Frozen Semen Diluter Mediaon The Plasma Membrane Intact, The Expression of Caspase and Malondialdehyde Post Thawing</title><creator>Hernawati, Tatik</creator><subject>SF600-1100 Veterinary medicine</subject><description>Background: The quality of frozen semen after thawing is one of factors in artificial in
semination. Cryopreservation affects the post-thawed sperm quality. The seminal
plasmadiary bull’s contains high concentration of specific proteinosteopontin. Our hypothesis
is that osteopontin maintain sperm quality during cryopreservation.
Aim :The aim was to investigate osteopontin does improve the quality of frozen semen as
appears from increased The plasma membraneintact ( PMI) , decreased expression of
caspase and Malondialdehyde (MDA)
Method : isolation, identification and protein specification of osteopontin from dairy
bullsemen. using SDS-PAGE method indicated that there were some tapes showing
differentmolecular weight from several samples. SDS-PAGE result from protein
identification wascontinued with protein identification using Western Blot technique. The
effect of osteopontinon the quality of frozen semen post thawing by the expression
ofcaspasewithimmunocytochemistry techniques andmeasurementof MDA
Result: of the first phase was determined that molecular weight protein specification of
osteopontin is 56 kDa. Osteopontin concentration from electroelution samples were analyzed
using Nano drop method, and it showed the protein concentrations as avaregely 180 μg /ml.
result of the second phase indicated that the addition of osteopontin can improve semen
quality through increased The plasma membraneintact ( PMI), decreased of expression
caspase 9 and MDA
Conclusion : Addition of osteopontin may improve semen quality through increased The
plasma membraneintact ( PMI) decreasedofexpressioncaspase9 and MDA
Key words: Spesifik Protein, Friesian Holstein diary bull, seminal plasma, PMI, MDA,
Caspase 9</description><publisher>Asian Academic Research Journal Associates</publisher><date>2016-04</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84618/1/Bukti%2003.%20Supplementation%20of%20Specific%20......pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84618/2/Peer%20Review%20Bukti%2003.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/84618/3/BUKTI%203%20SUPPLEMENTATION%20OF%20SPECIFIC%20PROTEINS%20IN%20THE%20SEMINAL%20PLASMA%20FROZEN%20SEMEN%20DILUTER%20MEDIAON%20THE%20PLASMA%20MEMBRANE%20INTACT%2C%20THE%20EXPRESSION%20OF%20CASPASE%20AND%20MALONDIALDEHYDE%20POST%20THAWING-compressed.pdf</identifier><identifier> Hernawati, Tatik (2016) Supplementation of Specific Proteins in The Seminal Plasma Frozen Semen Diluter Mediaon The Plasma Membrane Intact, The Expression of Caspase and Malondialdehyde Post Thawing. Asian Academic Research Journal of Multidisciplinary, 3 (4). pp. 105-116. ISSN 2319-2801 </identifier><relation>https://www.asianacademicresearch.org/2016_abstract/april_md_2016/12.pdf</relation><recordID>84618</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal PeerReview:PeerReviewed PeerReview Book:Book Book |
| author |
Hernawati, Tatik |
| title |
Supplementation of Specific Proteins in The Seminal Plasma Frozen Semen Diluter Mediaon The Plasma Membrane Intact, The Expression of Caspase and Malondialdehyde Post Thawing |
| publisher |
Asian Academic Research Journal Associates |
| publishDate |
2016 |
| topic |
SF600-1100 Veterinary medicine |
| url |
http://repository.unair.ac.id/84618/1/Bukti%2003.%20Supplementation%20of%20Specific%20......pdf http://repository.unair.ac.id/84618/2/Peer%20Review%20Bukti%2003.pdf http://repository.unair.ac.id/84618/3/BUKTI%203%20SUPPLEMENTATION%20OF%20SPECIFIC%20PROTEINS%20IN%20THE%20SEMINAL%20PLASMA%20FROZEN%20SEMEN%20DILUTER%20MEDIAON%20THE%20PLASMA%20MEMBRANE%20INTACT%2C%20THE%20EXPRESSION%20OF%20CASPASE%20AND%20MALONDIALDEHYDE%20POST%20THAWING-compressed.pdf http://repository.unair.ac.id/84618/ https://www.asianacademicresearch.org/2016_abstract/april_md_2016/12.pdf |
| contents |
Background: The quality of frozen semen after thawing is one of factors in artificial in
semination. Cryopreservation affects the post-thawed sperm quality. The seminal
plasmadiary bull’s contains high concentration of specific proteinosteopontin. Our hypothesis
is that osteopontin maintain sperm quality during cryopreservation.
Aim :The aim was to investigate osteopontin does improve the quality of frozen semen as
appears from increased The plasma membraneintact ( PMI) , decreased expression of
caspase and Malondialdehyde (MDA)
Method : isolation, identification and protein specification of osteopontin from dairy
bullsemen. using SDS-PAGE method indicated that there were some tapes showing
differentmolecular weight from several samples. SDS-PAGE result from protein
identification wascontinued with protein identification using Western Blot technique. The
effect of osteopontinon the quality of frozen semen post thawing by the expression
ofcaspasewithimmunocytochemistry techniques andmeasurementof MDA
Result: of the first phase was determined that molecular weight protein specification of
osteopontin is 56 kDa. Osteopontin concentration from electroelution samples were analyzed
using Nano drop method, and it showed the protein concentrations as avaregely 180 μg /ml.
result of the second phase indicated that the addition of osteopontin can improve semen
quality through increased The plasma membraneintact ( PMI), decreased of expression
caspase 9 and MDA
Conclusion : Addition of osteopontin may improve semen quality through increased The
plasma membraneintact ( PMI) decreasedofexpressioncaspase9 and MDA
Key words: Spesifik Protein, Friesian Holstein diary bull, seminal plasma, PMI, MDA,
Caspase 9 |
| id |
IOS3215.84618 |
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Universitas Airlangga |
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33 |
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library:university library |
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Perpustakaan Universitas Airlangga |
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468 |
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Airlangga Institutional Repositories |
| repository_id |
3215 |
| subject_area |
Adat Istiadat Administrasi Negara dan Militer Agama |
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KOTA SURABAYA |
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JAWA TIMUR |
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IOS3215 |
| first_indexed |
2019-07-21T18:18:42Z |
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2019-07-21T18:18:42Z |
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dc |
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1685906474425909248 |
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17.611513 |