Penerapan Metode Transfer Langsung Pada Kriopreservasi Embrio Sapi Perah
| Main Authors: | Supriatna, Iman, Yusuf, Tuty Laswardi, Purwantara, Bambang, Moekti, Gozali, Hernomoadi, Lies Parede |
|---|---|
| Terbitan: |
IPB (Bogor Agricultural University)
, 2010
|
| Online Access: |
http://repository.ipb.ac.id/handle/123456789/24829 |
| ctrlnum |
123456789-24829 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Penerapan Metode Transfer Langsung Pada Kriopreservasi Embrio Sapi Perah</title><title>The Use Of Direct Transfer Method On Embryo Cryopreservation In Dairy Cattle</title><creator>Supriatna, Iman</creator><creator>Yusuf, Tuty Laswardi</creator><creator>Purwantara, Bambang</creator><creator>Moekti, Gozali</creator><creator>Hernomoadi, Lies Parede</creator><description>direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-four morula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0.8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P<0.05). Following 24 hours ill vitro culture, there was no significant difference on the viability of thawed embryos cryoptotected with 1.5 M EG and rehydrated sing different concentration of sucrose (P>0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.8 M sucrose were 80.0, 80.8, 90.9 and 81.8%, respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0.8 M (90.0%) sucrose was significantly better compared to those without (22.2%~o) r with 0.2 M (36.34) sucrose (P<0.0 1). Key words: cryopreservation, direct transfer, cryoprotectant, ethylene glycol, propanediol, embryo</description><date>2010-05-24T04:43:22Z</date><date>2010-05-24T04:43:22Z</date><date>1999</date><identifier>http://repository.ipb.ac.id/handle/123456789/24829</identifier><publisher>IPB (Bogor Agricultural University)</publisher><recordID>123456789-24829</recordID></dc>
|
| author |
Supriatna, Iman Yusuf, Tuty Laswardi Purwantara, Bambang Moekti, Gozali Hernomoadi, Lies Parede |
| title |
Penerapan Metode Transfer Langsung Pada Kriopreservasi Embrio Sapi Perah |
| publisher |
IPB (Bogor Agricultural University) |
| publishDate |
2010 |
| url |
http://repository.ipb.ac.id/handle/123456789/24829 |
| contents |
direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-four morula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0.8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P<0.05). Following 24 hours ill vitro culture, there was no significant difference on the viability of thawed embryos cryoptotected with 1.5 M EG and rehydrated sing different concentration of sucrose (P>0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.8 M sucrose were 80.0, 80.8, 90.9 and 81.8%, respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0.8 M (90.0%) sucrose was significantly better compared to those without (22.2%~o) r with 0.2 M (36.34) sucrose (P<0.0 1). Key words: cryopreservation, direct transfer, cryoprotectant, ethylene glycol, propanediol, embryo |
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IOS3315.123456789-24829 |
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Institut Pertanian Bogor |
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20 |
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library:university library |
| library |
Perpustakaan Institut Pertanian Bogor |
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96 |
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IPB Scientific Repository |
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3315 |
| subject_area |
Kehutanan Pertanian Peternakan |
| city |
BOGOR |
| province |
JAWA BARAT |
| repoId |
IOS3315 |
| first_indexed |
2016-09-22T20:52:13Z |
| last_indexed |
2017-02-25T18:15:11Z |
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dc |
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1685918564866850816 |
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17.60897 |