Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line
| Main Authors: | Mutiah, Roihatul, Kristanti, Risma Aprinda, Susanti, Erna, Minggarwati, Trian Sidha |
|---|---|
| Format: | Journal PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
ISCC
, 2019
|
| Subjects: | |
| Online Access: |
http://repository.uin-malang.ac.id/4553/1/21.Jurnal%20bersama%20Trian.pdf http://repository.uin-malang.ac.id/4553/ https://ijcc.chemoprev.org/index.php/ijcc/article/view/294 http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss3pp131-139 |
| ctrlnum |
4553 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.uin-malang.ac.id/4553/</relation><title>Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line</title><creator>Mutiah, Roihatul</creator><creator>Kristanti, Risma Aprinda</creator><creator>Susanti, Erna</creator><creator>Minggarwati, Trian Sidha</creator><subject>111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified</subject><description>Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).</description><publisher>ISCC</publisher><date>2019</date><type>Journal:Journal</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><rights>cc_by_sa_4</rights><identifier>http://repository.uin-malang.ac.id/4553/1/21.Jurnal%20bersama%20Trian.pdf</identifier><identifier> Mutiah, Roihatul and Kristanti, Risma Aprinda and Susanti, Erna and Minggarwati, Trian Sidha (2019) Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line. Indonesian Journal of Cancer Chemoprevention, 10 (3). pp. 1-9. ISSN 2355-8989 </identifier><relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/294</relation><relation>http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss3pp131-139</relation><recordID>4553</recordID></dc>
|
| language |
eng |
| format |
Journal:Journal Journal PeerReview:PeerReviewed PeerReview Book:Book Book |
| author |
Mutiah, Roihatul Kristanti, Risma Aprinda Susanti, Erna Minggarwati, Trian Sidha |
| title |
Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| publisher |
ISCC |
| publishDate |
2019 |
| topic |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified |
| url |
http://repository.uin-malang.ac.id/4553/1/21.Jurnal%20bersama%20Trian.pdf http://repository.uin-malang.ac.id/4553/ https://ijcc.chemoprev.org/index.php/ijcc/article/view/294 http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss3pp131-139 |
| contents |
Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical). |
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Universitas Islam Negeri Maulana Malik Ibrahim Malang |
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