Molecular Characterization of Begomovirus on Cucumber in Java
| Main Authors: | Listihani, Listihani, Damayanti, Tri Asmira, Hidayat, Sri Hendrastuti, Wiyono, Suryo |
|---|---|
| Other Authors: | Indonesia Ministry of Research and Technology and Higher Education |
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Universitas Gadjah Mada
, 2019
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| Subjects: | |
| Online Access: |
https://jurnal.ugm.ac.id/jpti/article/view/41402 https://jurnal.ugm.ac.id/jpti/article/view/41402/26996 https://jurnal.ugm.ac.id/jpti/article/downloadSuppFile/41402/6452 |
| ctrlnum |
article-41402 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Molecular Characterization of Begomovirus on Cucumber in Java</title><creator>Listihani, Listihani</creator><creator>Damayanti, Tri Asmira</creator><creator>Hidayat, Sri Hendrastuti</creator><creator>Wiyono, Suryo</creator><subject lang="en-US">Begomovirus; cucumber; Java isolates; ToLCNDV</subject><description lang="en-US">A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java.</description><publisher lang="en-US">Universitas Gadjah Mada</publisher><contributor lang="en-US">Indonesia Ministry of Research and Technology and Higher Education</contributor><date>2019-12-03</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>https://jurnal.ugm.ac.id/jpti/article/view/41402</identifier><identifier>10.22146/jpti.41402</identifier><source lang="en-US">Jurnal Perlindungan Tanaman Indonesia; Vol 23, No 2 (2019); 197-204</source><source>2548-4788</source><source>1410-1637</source><language>eng</language><relation>https://jurnal.ugm.ac.id/jpti/article/view/41402/26996</relation><relation>https://jurnal.ugm.ac.id/jpti/article/downloadSuppFile/41402/6452</relation><rights lang="en-US">Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia</rights><rights lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</rights><recordID>article-41402</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:Journal |
| author |
Listihani, Listihani Damayanti, Tri Asmira Hidayat, Sri Hendrastuti Wiyono, Suryo |
| author2 |
Indonesia Ministry of Research and Technology and Higher Education |
| title |
Molecular Characterization of Begomovirus on Cucumber in Java |
| publisher |
Universitas Gadjah Mada |
| publishDate |
2019 |
| topic |
Begomovirus cucumber Java isolates ToLCNDV |
| url |
https://jurnal.ugm.ac.id/jpti/article/view/41402 https://jurnal.ugm.ac.id/jpti/article/view/41402/26996 https://jurnal.ugm.ac.id/jpti/article/downloadSuppFile/41402/6452 |
| contents |
A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java. |
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IOS3848.article-41402 |
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Universitas Gadjah Mada |
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19 |
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library:university library |
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Perpustakaan Pusat Universitas Gadjah Mada |
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Jurnal Perlindungan Tanaman Indonesia |
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3848 |
| subject_area |
General Topics of Pests and Diseases Control/Pembasmian Hama pada Tanaman Pertanian Animal Pests/Hama Binatang pada Tanaman Pertanian Plant Diseases/Penyakit pada Tanaman Pertanian |
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SLEMAN |
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DAERAH ISTIMEWA YOGYAKARTA |
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IOS3848 |
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2019-12-14T01:33:47Z |
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2020-03-22T13:08:44Z |
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