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Isolasi Metabolit Sekunder dari Jamur Endofitik Akar Tumbuhan Sambiloto (Andrographis paniculata Nees)

Main Authors: Elfita, Elfita, Munawar, Munawar
Other Authors: Raffiudin, Rika , Supena, Ence D J , Widyastuti, Utut , Purwantiningsih, Purwantiningsih, Sumaryada, Tony Ibnu , Sitanggang, Imas Sukaesih , Kusuma, Wisnu Ananta, Sulistyani, Sulistyani, Indahwati, Indahwati, Effendi, Sobri, Kusnanto, Ali
Format: BookSection PeerReviewed application/pdf
Terbitan: FMIPA IPB Bagor , 2014
Subjects:
QD Chemistry
Online Access: http://eprints.unsri.ac.id/5880/1/PROSIDING_SEMIRATA_IPB.pdf
http://eprints.unsri.ac.id/5880/
ctrlnum 5880
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Book
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Elfita, Elfita
Munawar, Munawar
author2 Raffiudin, Rika
Supena, Ence D J
Widyastuti, Utut
Purwantiningsih, Purwantiningsih
Sumaryada, Tony Ibnu
Sitanggang, Imas Sukaesih
Kusuma, Wisnu Ananta
Sulistyani, Sulistyani
Indahwati, Indahwati
Effendi, Sobri
Kusnanto, Ali
title Isolasi Metabolit Sekunder dari Jamur Endofitik Akar Tumbuhan Sambiloto (Andrographis paniculata Nees)
publisher FMIPA IPB Bagor
publishDate 2014
isbn 9786027049109
topic QD Chemistry
url http://eprints.unsri.ac.id/5880/1/PROSIDING_SEMIRATA_IPB.pdf
http://eprints.unsri.ac.id/5880/
contents Endophytic fungi are important sources of bioactive natural products with enormous potential for the discovery of new molecules for drug discovery, industrial use and agricultural applications. This study was conducted on the isolation of secondary metabolite from the endophytic fungi of selected plants sambiloto. Three endophytic fungi from root of sambiloto were isolated and purified. One of the Unidentified fungi (Sb-1) was cultured in 5L Potato Dextrose Broth (PDB) medium at room temperature (no shaking) for 8 weeks. The culture broth was filtered to remove mycelia. The culture media was then extracted in 5 L ethyl acetate (2 times), followed by filtration and then taken to evaporation. The extract was separated into the fractions by column chromatography (CC) on silica gel. The fractions was further separated by silica gel column chromatography to give one aromatic compound. The molecular structure was identified on the basis of spectroscopic UV analysis
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