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Isolation and Evaluation of The Antioxidant Activity of Phenolic Constituent of The Garcinia sizygiifolia

Main Authors: Muharni, Muharni, Elfita, Elfita, Pratama, Didi
Format: Article PeerReviewed application/pdf
Terbitan: FMIPA Kimia Unsoed , 2017
Subjects:
QD Chemistry
Online Access: http://eprints.unsri.ac.id/7419/1/Molekul_2017.pdf
http://eprints.unsri.ac.id/7419/
ctrlnum 7419
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format Journal:Article
Journal
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Muharni, Muharni
Elfita, Elfita
Pratama, Didi
title Isolation and Evaluation of The Antioxidant Activity of Phenolic Constituent of The Garcinia sizygiifolia
publisher FMIPA Kimia Unsoed
publishDate 2017
topic QD Chemistry
url http://eprints.unsri.ac.id/7419/1/Molekul_2017.pdf
http://eprints.unsri.ac.id/7419/
contents Garcinia sizygiifolia is a native plant to the South Sulawesi region popularly known as sula and has been cultivated in several regions in Indonesia. The plant by local community use as a food and source of wood, but has not found information on chemical content and biological activity. Therefore, this study was carried out to Isolation and evaluation of the antioxidant activity of the phenolic constituent of the G. sizygiifolia. A 30.0 g portion of ethyl acetate extract of the stembark G. sizygiifolia were separated by column chromatography method using silica G 60 F254 (230-400 mesh), eluted gradient polarity mixtures of n-hexane-ethyl acetate were collected and sorted into fractions. Fraction F 1 (5.2 g) were separated and purified again by chromatography method until pure compound obtained. The structure of the isolated compound was determined using UV, IR, and NMR spectroscopy. The antioxidant activity was tested by DPPH method. The isolated pure compound was a yellow solid with melting point 148-149 oC. Base on spectroscopy data and by comparison with data from the literature, isolated compound is a known compound 2,4-dihydroxyphenylethanone. The compound exhibited antioxidant activity with IC50 96 μg/mL against DPPH.
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