Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)

Main Authors: Herdis, Herdis, Surachman, Maman, Rizal, Muhammad, Boediono, Arief, Yulnawati, Yulnawati
Format: Article info application/pdf eJournal
Bahasa: ind
Terbitan: Fakultas Biologi | Universitas Jenderal Soedirman , 2006
Subjects:
Online Access: https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142
https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142/102
ctrlnum article-142
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)</title><creator>Herdis, Herdis</creator><creator>Surachman, Maman</creator><creator>Rizal, Muhammad</creator><creator>Boediono, Arief</creator><creator>Yulnawati, Yulnawati</creator><subject lang="id-ID">Trehalose, Tris extender, chilled-cement, Garut ram</subject><description lang="id-ID">This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations.&#xA0; Cement was collected from three mature rams using an artificial vagina once a week.&#xA0; After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively.&#xA0; The extended-cement was stored at 5oC.&#xA0; Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days.&#xA0; The&#xA0; results showed that addition of trehalose did not significantly improved&#xA0; the percentage of sperm motility, live sperm, and IPM (P&gt;0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days.&#xA0; In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.</description><publisher lang="id-ID">Fakultas Biologi | Universitas Jenderal Soedirman</publisher><contributor lang="id-ID"/><date>2006-01-10</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>Other:</type><type>File:application/pdf</type><identifier>https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142</identifier><identifier>10.20884/1.mib.2006.23.1.142</identifier><source lang="en-US">Biosfera; Vol 23, No 1 (2006); 24-30</source><source lang="id-ID">Biosfera; Vol 23, No 1 (2006); 24-30</source><source>2528-2050</source><source>0853-1625</source><language>ind</language><relation>https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142/102</relation><rights lang="id-ID">http://creativecommons.org/licenses/by/4.0</rights><recordID>article-142</recordID></dc>
language ind
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
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Other:
File:application/pdf
File
Journal:eJournal
author Herdis, Herdis
Surachman, Maman
Rizal, Muhammad
Boediono, Arief
Yulnawati, Yulnawati
title Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)
publisher Fakultas Biologi | Universitas Jenderal Soedirman
publishDate 2006
topic Trehalose
Tris extender
chilled-cement
Garut ram
url https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142
https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/142/102
contents This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations. Cement was collected from three mature rams using an artificial vagina once a week. After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively. The extended-cement was stored at 5oC. Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days. The results showed that addition of trehalose did not significantly improved the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days. In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.
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