Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda

Main Authors: Susanto, Agus, Nuniek, Nuniek Ina, Ekowati, Nuraeni
Other Authors: Fak Biologi Unsoed
Format: Article info application/pdf eJournal
Bahasa: ind
Terbitan: Fakultas Biologi | Universitas Jenderal Soedirman , 2018
Subjects:
Online Access: https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566
https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566/pdf
ctrlnum article-566
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda</title><creator>Susanto, Agus</creator><creator>Nuniek, Nuniek Ina</creator><creator>Ekowati, Nuraeni</creator><subject lang="id-ID">Coprinus comatus, Extraction, Antioxidant, DPPH Method</subject><description lang="id-ID">Coprinus comatus or with local name is&#xA0; drum stick chiken mushroom that includes edible and medicinal mushroom. This mushroom contains bioactive compounds that have several pharmacological effects, like as antibacterial, anticancer, immunomodulator, anti-inflammatory, prevent diabetes and antioxidant. bioactive compounds includes in fruit body C. comatus can be obtained by extraction used solvent. The objective of this study was to determine whether the C. comatus fungus was potential as an antioxidant producer and to know which solvent produced the best antioxidant activity from the extract of the fruit body of C. comatus. The method used in this research is experimental with two treatments ie n-hexane and ethanol 70% solvent. Fruit body of C. comatus is extracted by maceration method, then the viscous extract obtained by phytochemical test and antioxidant activity test using DPPH method (1,1-diphenyl-2-picrihidazil). The results obtained are then analyzed using t-test for independent samples. The results showed that the average yield of ethanol extract 70% higher than n-heksan &#xA0;were each 61.33% &#xB1; 9,21 and 14.8% &#xB1; 0,87. The test of total phenol extract ethanol was 70% higher than n-hexane , each with an average of 325,19 ppm &#xB1; 50,01 and 110,08 ppm &#xB1; 34,67. The result of antioxidant activity test using DPPH method of ethanol extract 70% and n-heksan has IC50 value 2,48 mg/ml and 3,86 mg/ml. Phytochemical test results for flavonoid test showed positive result for both samples extract, terpenoid test showed positive result in both samples , the tannin test showed negative results &#xA0;in both samples.</description><publisher lang="id-ID">Fakultas Biologi | Universitas Jenderal Soedirman</publisher><contributor lang="id-ID">Fak Biologi Unsoed</contributor><date>2018-05-01</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>Other:</type><type>File:application/pdf</type><identifier>https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566</identifier><identifier>10.20884/1.mib.2018.35.2.566</identifier><source lang="en-US">Majalah Ilmiah Biologi Biosfera: A Scientific Journal; Vol 35, No 2 (2018); 63-68</source><source lang="id-ID">Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal; Vol 35, No 2 (2018); 63-68</source><source>2528-2050</source><source>0853-1625</source><language>ind</language><relation>https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566/pdf</relation><rights lang="id-ID">Hak Cipta (c) 2018 Nuraeni Ekowati</rights><rights lang="id-ID">http://creativecommons.org/licenses/by/4.0</rights><recordID>article-566</recordID></dc>
language ind
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
File:application/pdf
File
Journal:eJournal
author Susanto, Agus
Nuniek, Nuniek Ina
Ekowati, Nuraeni
author2 Fak Biologi Unsoed
title Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda
publisher Fakultas Biologi | Universitas Jenderal Soedirman
publishDate 2018
topic Coprinus comatus
Extraction
Antioxidant
DPPH Method
url https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566
https://journal.bio.unsoed.ac.id/index.php/biosfera/article/view/566/pdf
contents Coprinus comatus or with local name is drum stick chiken mushroom that includes edible and medicinal mushroom. This mushroom contains bioactive compounds that have several pharmacological effects, like as antibacterial, anticancer, immunomodulator, anti-inflammatory, prevent diabetes and antioxidant. bioactive compounds includes in fruit body C. comatus can be obtained by extraction used solvent. The objective of this study was to determine whether the C. comatus fungus was potential as an antioxidant producer and to know which solvent produced the best antioxidant activity from the extract of the fruit body of C. comatus. The method used in this research is experimental with two treatments ie n-hexane and ethanol 70% solvent. Fruit body of C. comatus is extracted by maceration method, then the viscous extract obtained by phytochemical test and antioxidant activity test using DPPH method (1,1-diphenyl-2-picrihidazil). The results obtained are then analyzed using t-test for independent samples. The results showed that the average yield of ethanol extract 70% higher than n-heksan were each 61.33% ± 9,21 and 14.8% ± 0,87. The test of total phenol extract ethanol was 70% higher than n-hexane , each with an average of 325,19 ppm ± 50,01 and 110,08 ppm ± 34,67. The result of antioxidant activity test using DPPH method of ethanol extract 70% and n-heksan has IC50 value 2,48 mg/ml and 3,86 mg/ml. Phytochemical test results for flavonoid test showed positive result for both samples extract, terpenoid test showed positive result in both samples , the tannin test showed negative results in both samples.
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institution Universitas Jenderal Soedirman
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