PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN [Vegetative Propagation of Chrysant by Tissue Culture Technique]

Main Authors: Dwimahyani, Ita, Gandanegara, S
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Research Center for Biology-Indonesian Institute of Sciences , 2016
Subjects:
Online Access: http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127/1002
ctrlnum article-1127
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN [Vegetative Propagation of Chrysant by Tissue Culture Technique]</title><creator>Dwimahyani, Ita</creator><creator>Gandanegara, S</creator><subject lang="en-US">Perbanyakan vegetatif/ vegetatively propagated, kultur jaringan/ tissue culture, krisan/ chrysant, Chrysanthemum morifolium.</subject><description lang="en-US">Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.</description><publisher lang="en-US">Research Center for Biology-Indonesian Institute of Sciences</publisher><contributor lang="en-US"/><date>2016-02-25</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127</identifier><identifier>10.14203/beritabiologi.v5i4.1127</identifier><source lang="en-US">BERITA BIOLOGI; Vol 5, No 4 (2001)</source><source lang="id-ID">BERITA BIOLOGI; Vol 5, No 4 (2001)</source><language>eng</language><relation>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127/1002</relation><rights lang="en-US">Copyright (c) 2015 BERITA BIOLOGI</rights><recordID>article-1127</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
File:application/pdf
File
Journal:eJournal
author Dwimahyani, Ita
Gandanegara, S
title PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN [Vegetative Propagation of Chrysant by Tissue Culture Technique]
publisher Research Center for Biology-Indonesian Institute of Sciences
publishDate 2016
topic Perbanyakan vegetatif/ vegetatively propagated
kultur jaringan/ tissue culture
krisan/ chrysant
Chrysanthemum morifolium
url http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127/1002
contents Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.
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institution Lembaga Ilmu Pengetahuan Indonesia
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library Perpustakaan Pusat Penelitian Biologi-LIPI
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subject_area Biologi
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province JAWA BARAT
repoId IOS5356
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