RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS
| Main Authors: | Iskandariah, Iskandariah, Soelistyowati, Dinar Tri, Gustiano, Rudhy, Kusmini, Irin Iriana, Huwoyon, Gleni Hasan |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Research Center for Biology-Indonesian Institute of Sciences
, 2016
|
| Subjects: | |
| Online Access: |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866/1751 |
| ctrlnum |
article-1866 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS</title><creator>Iskandariah, Iskandariah</creator><creator>Soelistyowati, Dinar Tri</creator><creator>Gustiano, Rudhy</creator><creator>Kusmini, Irin Iriana</creator><creator>Huwoyon, Gleni Hasan</creator><subject lang="en-US">Genetics, Kalimantan, morphometric truss, RAPD, Trichopodus pectoralis.</subject><description lang="en-US">The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.</description><publisher lang="en-US">Research Center for Biology-Indonesian Institute of Sciences</publisher><contributor lang="en-US"/><date>2016-01-14</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866</identifier><identifier>10.14203/beritabiologi.v14i1.1866</identifier><source lang="en-US">BERITA BIOLOGI; Vol 14, No 1 (2015); 57 – 68</source><source lang="id-ID">BERITA BIOLOGI; Vol 14, No 1 (2015); 57 – 68</source><source>2337-8751</source><source>0126-1754</source><language>eng</language><relation>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866/1751</relation><rights lang="en-US">Copyright (c) 2016 BERITA BIOLOGI</rights><recordID>article-1866</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Iskandariah, Iskandariah Soelistyowati, Dinar Tri Gustiano, Rudhy Kusmini, Irin Iriana Huwoyon, Gleni Hasan |
| title |
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS |
| publisher |
Research Center for Biology-Indonesian Institute of Sciences |
| publishDate |
2016 |
| topic |
Genetics Kalimantan morphometric truss RAPD Trichopodus pectoralis |
| url |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1866/1751 |
| contents |
The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan. |
| id |
IOS5356.article-1866 |
| institution |
Lembaga Ilmu Pengetahuan Indonesia |
| institution_id |
58 |
| institution_type |
library:special library |
| library |
Perpustakaan Pusat Penelitian Biologi-LIPI |
| library_id |
983 |
| collection |
Berita Biologi |
| repository_id |
5356 |
| subject_area |
Biologi |
| city |
Cibinong |
| province |
JAWA BARAT |
| repoId |
IOS5356 |
| first_indexed |
2018-03-14T21:58:46Z |
| last_indexed |
2019-05-08T13:18:39Z |
| recordtype |
dc |
| _version_ |
1686341578918985728 |
| score |
17.60506 |