SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH

Main Authors: Utami, Dwinita W, Ilhami, Aderahma, Hanarida, Ida
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Research Center for Biology-Indonesian Institute of Sciences , 2016
Subjects:
Online Access: http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966/1845
ctrlnum article-1966
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH</title><creator>Utami, Dwinita W</creator><creator>Ilhami, Aderahma</creator><creator>Hanarida, Ida</creator><subject lang="en-US">DNA fingerprinting, local red rice germplasm, molecular markers</subject><description lang="en-US">Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.</description><publisher lang="en-US">Research Center for Biology-Indonesian Institute of Sciences</publisher><contributor lang="en-US"/><date>2016-02-17</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966</identifier><identifier>10.14203/beritabiologi.v10i2.1966</identifier><source lang="en-US">BERITA BIOLOGI; Vol 10, No 2 (2010); 143 &#x2013; 150</source><source lang="id-ID">BERITA BIOLOGI; Vol 10, No 2 (2010); 143 &#x2013; 150</source><source>2337-8751</source><source>0126-1754</source><language>eng</language><relation>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966/1845</relation><rights lang="en-US">Copyright (c) 2016 BERITA BIOLOGI</rights><recordID>article-1966</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
File:application/pdf
File
Journal:eJournal
author Utami, Dwinita W
Ilhami, Aderahma
Hanarida, Ida
title SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH
publisher Research Center for Biology-Indonesian Institute of Sciences
publishDate 2016
topic DNA fingerprinting
local red rice germplasm
molecular markers
url http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1966/1845
contents Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.
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institution Lembaga Ilmu Pengetahuan Indonesia
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