KARAKTERISASI BAKTERI PENGHASIL a-AMILASE DAN IDENTIFIKASI ISOLAT C2 YANG DIISOLASI DARI TERASI CURAH SAMARINDA, KALMANTAN TIMUR
| Main Author: | Soeka, Yati Sodaryati |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Research Center for Biology-Indonesian Institute of Sciences
, 2017
|
| Subjects: | |
| Online Access: |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290/2437 |
| ctrlnum |
article-2290 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">KARAKTERISASI BAKTERI PENGHASIL a-AMILASE DAN IDENTIFIKASI ISOLAT C2 YANG DIISOLASI DARI TERASI CURAH SAMARINDA, KALMANTAN TIMUR</title><creator>Soeka, Yati Sodaryati</creator><subject lang="en-US">shrimp-paste, ?-amylase, enzyme activity, characterization, Bacillus subtilis</subject><description lang="en-US">Enzymes a-amylase is one of the enzymes used in process of starch degradation. This present study was aimed to characterize and identify of a-amylase producing strain isolated from bulk shrimp-paste in Samarinda, East Kalimantan was carried out. An experiment was conducted to examine influences of incubation, carbon source, substrate concentration temperature for incubation, pH of media, and addition of metal ions. Identification of strain C2 was carried out by using Wizard Genomic DNA Purification Kit (Promega). The result showed that optimum activity of a-amylase from C2 after six days incubation was 18.93 U/mL. Tests on the type of substrate , soluble starch was the best source to produce a- amylase (14.51 U/mL). However, at concentration of 2 % and incubation temperature at 40°C, enzymatic activity was decreased to 12.56 U/mL and 12.79 U/mL, respectively within residual activity of 74.75%. The enzyme activity was 16.43 U/mL and its residual activity was 39.14 % when it was assayed at pH 8.5. Addition of metal ions in the form of divalent and monovalent cations (1 mM) showed that the enzyme could be activated by ion Ca2+ while ion Cu2+, Co2+, Mg2+, Zn2+ , Na+  decrease the activity of the enzyme. Identification of strain C2 using molecular characterization demonstrated that partial sequences of 16S rDNA reffered to as Bacillus subtilis.</description><publisher lang="en-US">Research Center for Biology-Indonesian Institute of Sciences</publisher><contributor lang="en-US"/><date>2017-01-18</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290</identifier><identifier>10.14203/beritabiologi.v15i2.2290</identifier><source lang="en-US">BERITA BIOLOGI; Vol 15, No 2 (2016); 185 – 193</source><source lang="id-ID">BERITA BIOLOGI; Vol 15, No 2 (2016); 185 – 193</source><source>2337-8751</source><source>0126-1754</source><language>eng</language><relation>http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290/2437</relation><rights lang="en-US">Copyright (c) 2017 BERITA BIOLOGI</rights><recordID>article-2290</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Soeka, Yati Sodaryati |
| title |
KARAKTERISASI BAKTERI PENGHASIL a-AMILASE DAN IDENTIFIKASI ISOLAT C2 YANG DIISOLASI DARI TERASI CURAH SAMARINDA, KALMANTAN TIMUR |
| publisher |
Research Center for Biology-Indonesian Institute of Sciences |
| publishDate |
2017 |
| topic |
shrimp-paste ?-amylase enzyme activity characterization Bacillus subtilis |
| url |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2290/2437 |
| contents |
Enzymes a-amylase is one of the enzymes used in process of starch degradation. This present study was aimed to characterize and identify of a-amylase producing strain isolated from bulk shrimp-paste in Samarinda, East Kalimantan was carried out. An experiment was conducted to examine influences of incubation, carbon source, substrate concentration temperature for incubation, pH of media, and addition of metal ions. Identification of strain C2 was carried out by using Wizard Genomic DNA Purification Kit (Promega). The result showed that optimum activity of a-amylase from C2 after six days incubation was 18.93 U/mL. Tests on the type of substrate , soluble starch was the best source to produce a- amylase (14.51 U/mL). However, at concentration of 2 % and incubation temperature at 40°C, enzymatic activity was decreased to 12.56 U/mL and 12.79 U/mL, respectively within residual activity of 74.75%. The enzyme activity was 16.43 U/mL and its residual activity was 39.14 % when it was assayed at pH 8.5. Addition of metal ions in the form of divalent and monovalent cations (1 mM) showed that the enzyme could be activated by ion Ca2+ while ion Cu2+, Co2+, Mg2+, Zn2+ , Na+ decrease the activity of the enzyme. Identification of strain C2 using molecular characterization demonstrated that partial sequences of 16S rDNA reffered to as Bacillus subtilis. |
| id |
IOS5356.article-2290 |
| institution |
Lembaga Ilmu Pengetahuan Indonesia |
| institution_id |
58 |
| institution_type |
library:special library |
| library |
Perpustakaan Pusat Penelitian Biologi-LIPI |
| library_id |
983 |
| collection |
Berita Biologi |
| repository_id |
5356 |
| subject_area |
Biologi |
| city |
Cibinong |
| province |
JAWA BARAT |
| repoId |
IOS5356 |
| first_indexed |
2018-03-14T21:58:42Z |
| last_indexed |
2019-05-08T13:19:03Z |
| recordtype |
dc |
| _version_ |
1686341578781622272 |
| score |
17.607244 |