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PERBANDINGAN NILAI DIAGNOSTIK IGE SPESIFIK TUNGAU DEBU RUMAH, METODE ELISA DAN IMUNOBLOT PADA RINITIS ALERGI

Main Authors: Habsari, Janti Tri, Aryati, Aryati, Pawarti, Dwi Reno
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA , 2018
Subjects:
Allergic rhinitis
specific IgE due to HDM
diagnostic value
ELISA
immunoblot
Online Access: http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/1113
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/1113/834
ctrlnum article-1113
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language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
File:application/pdf
File
Journal:eJournal
author Habsari, Janti Tri
Aryati, Aryati
Pawarti, Dwi Reno
title PERBANDINGAN NILAI DIAGNOSTIK IGE SPESIFIK TUNGAU DEBU RUMAH, METODE ELISA DAN IMUNOBLOT PADA RINITIS ALERGI
publisher PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
publishDate 2018
topic Allergic rhinitis
specific IgE due to HDM
diagnostic value
ELISA
immunoblot
url http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/1113
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/1113/834
contents The detection of allergen types is very helpful in allergic rhinitis (AR) management. Some methods had been performed to examinethe specific IgE due to HDM such as ELISA and immunoblot methods. The aim of this research is to know the difference of specificIgE diagnostic value due to HDM between ELISA and immunoblot in allergic rhinitis method which is expected to be used as in vitroalternative method which is safe, fast, effective, with a high sensitivity and specificity by provement. The samples were allergic rhinitisand non-allergic rhinitis patients at ENT of Head and Neck Out patients Clinic of Dr. Soetomo Hospital Surabaya. The sera was examinedfor specific IgE due to HDM by ELISA and immunoblot methods and then analyzed for its diagnostic value using the 2 x 2 table with a95% confidence interval. The comparation between both methods were analyzed with Wilcoxon test. The diagnostic value of the specificHDM IgE with immunoblot method showed sensitivity of 90% and 80% specificity, positive predictive value 90% and the negative 80%and diagnostic efficiency 86.67%. The positive likelihood ratio 4.5 and the negative one 0.125. The diagnostic value of the specific IgEHDM/D.p with ELISA showed a sensitivity of 75% and specificity 75%, the positive predictive value 85.71% and the negative one 0%and diagnostic efficiency 75%. The positive likelihood ratio was 3 and the negative one 0.33. The diagnostic value of the specific IgEHDM with immunoblot showed a sensitivity of 90% and specificity 80%, the positive predictive value 90% and the negative one 80%and the diagnostic efficiency 86.67%. The positive likelihood ratio was 4.5 and the negative one 0.125. The difference of diagnostic valuein both methods revealed that the p value was 0.013. It can be concluded in this study that there was a significant difference of specificIgE due to HDM between ELISA and immunoblot methods in allergic rhinitis.
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institution Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
institution_id 1807
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library Indonesian Journal of Clinical Pathology and Medical Laboratory
library_id 1493
collection Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
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subject_area Patologi Klinik
city KOTA SURABAYA
province JAWA TIMUR
repoId IOS5364
first_indexed 2018-04-01T07:53:16Z
last_indexed 2018-04-01T07:53:16Z
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