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DETEKSI CLOSTRIDIUM DIFFICILE TOKSIGENIK MENGGUNAKAN UJI CEPAT TOKSIN DAN REAL TIME POLYMERASE CHAIN REACTION

Main Authors: Yanti, Ika Yasma, Astrawinata, Dalima Ari Wahono
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA , 2016
Subjects:
Toxigenic clostridium difficile
toxin rapid test
real-time PCR
sensitivity
specificity
Online Access: http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153/pdf
ctrlnum article-153
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">DETEKSI CLOSTRIDIUM DIFFICILE TOKSIGENIK MENGGUNAKAN UJI CEPAT TOKSIN DAN REAL TIME POLYMERASE CHAIN REACTION</title><creator>Yanti, Ika Yasma</creator><creator>Astrawinata, Dalima Ari Wahono</creator><subject lang="en-US">Toxigenic clostridium difficile;toxin rapid test;real-time PCR;sensitivity;specificity</subject><description lang="en-US">Toxigenic Clostridium difficile infection, causing a Pseudo Membrane Colitis (PMC) and Clostridium Difficile Associated Diarrhea(CDAD) has increased sharply. The largest risk factor is the use of antibiotics. The purpose of this study was to know how to determinethe prevalence and characteristics of subjects with Toxigenic Clostridium difficile and to assess the ability of the toxin rapid test comparedto real-time PCR. Ninety adult subjects with antibiotic therapy more than two (2) weeks were enrolled in this study. The results of toxinrapid test and real-time PCR were presented in a 2x2 table, statistical test used was Chi square. The prevalence of Toxigenic Clostridiumdifficile based on the toxin rapid test and by real-time PCR was 27.3% and 37.5%, respectively. There were significant differences betweenstool consistency and number of antibiotics used with the detection of Toxigenic Clostridium difficile. There was a relationship betweenthe duration of antibiotic therapy with the detection of Toxigenic Clostridium difficile using real-time PCR (p=0.010, RR=2.116). Thesensitivity, specificity, PPV, NPV, PLR and NLR rapid test against real-time PCR were 69.7%; 98.2%; 95.8%; 84.4%; 39.2 and 0.31,respectively. This study concluded that the prevalence of Clostridium difficile in RSCM was higher compared to that in Malaysia, Thailandand India; the subjects with antibiotic therapy for more than four (4) weeks had a double risk to have Toxigenic Clostridium difficilethan subjects with antibiotic therapy for less than that time (4 weeks). Thus, in this study, toxin rapid test could be used as a tool todetect Toxigenic Clostridium difficile.</description><publisher lang="en-US">PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA</publisher><contributor lang="en-US"/><date>2016-09-13</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153</identifier><identifier>10.24293/ijcpml.v22i1.153</identifier><source lang="en-US">INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY; Vol 22, No 1 (2015); 22-26</source><source>2477-4685</source><source>0854-4263</source><source>10.24293/ijcpml.v22i1</source><language>eng</language><relation>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153/pdf</relation><rights lang="en-US">Copyright (c) 2016 Indonesian Journal of Clinical Pathology and Medical Laboratory</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc-nd/4.0</rights><recordID>article-153</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
File:application/pdf
File
Journal:eJournal
author Yanti, Ika Yasma
Astrawinata, Dalima Ari Wahono
title DETEKSI CLOSTRIDIUM DIFFICILE TOKSIGENIK MENGGUNAKAN UJI CEPAT TOKSIN DAN REAL TIME POLYMERASE CHAIN REACTION
publisher PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
publishDate 2016
topic Toxigenic clostridium difficile
toxin rapid test
real-time PCR
sensitivity
specificity
url http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/153/pdf
contents Toxigenic Clostridium difficile infection, causing a Pseudo Membrane Colitis (PMC) and Clostridium Difficile Associated Diarrhea(CDAD) has increased sharply. The largest risk factor is the use of antibiotics. The purpose of this study was to know how to determinethe prevalence and characteristics of subjects with Toxigenic Clostridium difficile and to assess the ability of the toxin rapid test comparedto real-time PCR. Ninety adult subjects with antibiotic therapy more than two (2) weeks were enrolled in this study. The results of toxinrapid test and real-time PCR were presented in a 2x2 table, statistical test used was Chi square. The prevalence of Toxigenic Clostridiumdifficile based on the toxin rapid test and by real-time PCR was 27.3% and 37.5%, respectively. There were significant differences betweenstool consistency and number of antibiotics used with the detection of Toxigenic Clostridium difficile. There was a relationship betweenthe duration of antibiotic therapy with the detection of Toxigenic Clostridium difficile using real-time PCR (p=0.010, RR=2.116). Thesensitivity, specificity, PPV, NPV, PLR and NLR rapid test against real-time PCR were 69.7%; 98.2%; 95.8%; 84.4%; 39.2 and 0.31,respectively. This study concluded that the prevalence of Clostridium difficile in RSCM was higher compared to that in Malaysia, Thailandand India; the subjects with antibiotic therapy for more than four (4) weeks had a double risk to have Toxigenic Clostridium difficilethan subjects with antibiotic therapy for less than that time (4 weeks). Thus, in this study, toxin rapid test could be used as a tool todetect Toxigenic Clostridium difficile.
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institution Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
institution_id 1807
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library Indonesian Journal of Clinical Pathology and Medical Laboratory
library_id 1493
collection Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
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subject_area Patologi Klinik
city KOTA SURABAYA
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repoId IOS5364
first_indexed 2018-03-14T22:04:43Z
last_indexed 2018-03-14T22:04:55Z
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