Effect of Chitin Addition to the Growth of Entomopathogenic fungi Penicillium sp

Main Authors: Nurariaty, Agus, Ade, Sugiarti
Bahasa: eng
Terbitan: , 2014
Subjects:
PDA
Online Access: http://repository.unhas.ac.id/handle/123456789/11264
Daftar Isi:
  • Penicillium sp. is one of the potentially entomopathogenic fungi to control the cacao pod borer (CPB). The study aims to determine the effect of chitin addition to the growth of entomopathogenic fungi Penicillium sp. Experiments was conducted in Pests Identification and Biological Control laboratory, Department of Plant Pest and Disease, Faculty of Agriculture, Hasanuddin University. The method was conducted that if the fungus Penicillium sp. has grown on PDA, then put in erlemeyer containing liquid medium were added chitin and the other without chitin as treatment. Observations was conducted on the growth of the fungus from 3, 6, 9 and 12 days by counting the number of spores / ml, wet weigh (g) and dry weight (g). The results showed that on the third day the number of spores of Penicillium sp. was higher on media were added chitin than without chitin. i.e. 3:48 x 106 and 0.159 x 106 spores / ml while on the 12th day i.e. 10.9 x 107 and 0,024 x 107 spores / ml. Similarly, that wet weight was higher when added chitin than without chitin i.e. 9.61 g and 5.35 g and the dry weight was 6.65 g and 4.99 g respectively..